Artículo
Abstract:
The accumulation and output of 3H -prostaglandins (PGs), E2 and F2α, into and from uterine strips isolated from ovariectomized rats, either in presence or in absence of exogenous progesterone, were explored. Tissue-to-medium ratio of 3H - counts (T/M-ratio), was determined. The same was done in solutions containing 14C-sucrose. During a 60 min incubation period in a solution containing 3H -PGF2α, a net accumulation of radioactivity was evident in control (no progesterone) uterine slices. The T/M-ratio for 3H-PGF2α, increased with time, reaching maximal values at 45 min. Progesterone (100 ug.ml-1) attenuated the uptake process, as evidenced by stable values of T/M-ratio, as time progressed. On the other hand, control T/M-ratio for fluenced by the presence of exogenous progesterone. Regarding labelled PG release from the tissue, it was observed that, during an experimental period of 60 min, most tritium from control slices was released within the first 30 min after incubation with 3H -PGF2α, whereas, following the presence and subsequent removal of exogenous progesterone, the bulk of 3H -released took place at 6-70 min. On the other hand, the release of 3H after an incubation with 3H -PGE2, was also maximal as that for 3H -PGF2, α within the first 30 min and resulted not altered after a period of exposure and removal of progesterone. The foregoing results suggest an specific pharmacological effect of progesterone, attenuating the uptake and retarding the outflow of PGF2α, but not that of PGE2, into and from uterine slices of ovariectomized rats. Findings reported herein are discussed in terms of progesterone priming and withdrawal, in relation to PGF2α fluxes in the rat uterus during the sex cycle, as well as in relation to PG binding to tissue receptors. © 1987.
Registro:
| Documento: | Artículo |
| Título: | Uptake and release of 3H prostaglandins E2 and F2α in uterin strips isolated from ovariectomized rats. Influence of in vitro progesterone |
| Autor: | Gimeno, M.F.; Franchi, A.M.; González, E.T.; Gimeno, A.L. |
| Filiación: | Centro de Estudios Farmacológicos y de Principios Naturales (CEFAPRIN). Serrano 665, 1414 Buenos Aires, Argentina Consejo Nacional de Investigaciones Cientiticas y Tecnicas de la Republica Argentina (CONICET). Serr, 1414 Buenos Aires, Argentina |
| Palabras clave: | progesterone; prostaglandin E; prostaglandin E2; prostaglandin F; prostaglandin F2 alpha; progesterone; prostaglandin e2; prostaglandin f2 alpha; radioisotope; sucrose c 14; unclassified drug; animal; article; female; in vitro study; metabolism; ovariectomy; rat; uterus; animal cell; drug administration; drug efficacy; drug mechanism; drug receptor binding; etiology; female genital system; muscle; nonhuman; priority journal; prostaglandin e2 h 3; prostaglandin f2 alpha h 3; sex difference; sexual cycle; uterus; Animal; Dinoprost; Dinoprostone; Female; In Vitro; Ovariectomy; Progesterone; Prostaglandins E; Prostaglandins F; Rats; Support, Non-U.S. Gov't; Uterus |
| Año: | 1987 |
| Volumen: | 33 |
| Número: | 1 |
| Página de inicio: | 51 |
| Página de fin: | 61 |
| DOI: | http://dx.doi.org/10.1016/0090-6980(87)90304-2 |
| Título revista: | Prostaglandins |
| Título revista abreviado: | Prostaglandins |
| ISSN: | 00906980 |
| CODEN: | PRGLB |
| CAS: | progesterone, 57-83-0; prostaglandin E, 11042-70-9; prostaglandin E2, 363-24-6; prostaglandin F2 alpha, 551-11-1; Dinoprost, 551-11-1; Dinoprostone, 363-24-6; Progesterone, 57-83-0; Prostaglandins E; Prostaglandins F |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00906980_v33_n1_p51_Gimeno |
Referencias:
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- Franchi, González, Gimeno, Gimeno, Progesterone enhances out put of prostaglandin F2-alpha by uterine horns isolated from adult ovariectomized and immature rats (1982) Prostaglandins, 24, p. 775
- Gimeno, Franchi, González, Gimeno, Progesterone in vitro and the output of prostaglandin F from the uterus of ovariectomized rats. Priming or withdeawal? (1984) Prostaglandins, Leukotrienes and Medicine, 14, p. 289
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Citas:
---------- APA ----------
Gimeno, M.F., Franchi, A.M., González, E.T. & Gimeno, A.L. (1987) . Uptake and release of 3H prostaglandins E2 and F2α in uterin strips isolated from ovariectomized rats. Influence of in vitro progesterone. Prostaglandins, 33(1), 51-61.http://dx.doi.org/10.1016/0090-6980(87)90304-2
---------- CHICAGO ----------
Gimeno, M.F., Franchi, A.M., González, E.T., Gimeno, A.L. "Uptake and release of 3H prostaglandins E2 and F2α in uterin strips isolated from ovariectomized rats. Influence of in vitro progesterone" . Prostaglandins 33, no. 1 (1987) : 51-61.http://dx.doi.org/10.1016/0090-6980(87)90304-2
---------- MLA ----------
Gimeno, M.F., Franchi, A.M., González, E.T., Gimeno, A.L. "Uptake and release of 3H prostaglandins E2 and F2α in uterin strips isolated from ovariectomized rats. Influence of in vitro progesterone" . Prostaglandins, vol. 33, no. 1, 1987, pp. 51-61.http://dx.doi.org/10.1016/0090-6980(87)90304-2
---------- VANCOUVER ----------
Gimeno, M.F., Franchi, A.M., González, E.T., Gimeno, A.L. Uptake and release of 3H prostaglandins E2 and F2α in uterin strips isolated from ovariectomized rats. Influence of in vitro progesterone. Prostaglandins. 1987;33(1):51-61.http://dx.doi.org/10.1016/0090-6980(87)90304-2
