Abstract:
Formation of {3H}-PGF2α and {3H}-13,14,dihydro-15-keto-PGF2α from {3H}-PGE2 by the supernatant of uterine homogenates from estrous and ovariectomized rats, was studied, using the reaction system PGE2 + NADPH + {3H}-PGE2 + supernatant. Enzymatic conversion was lower in uterine supernatants from spayed rats than in uterine homogenates of rats at natural estrus. Spayed animals were injected with progesterone (P) or with estradiol-17-β (E0) at a dose of 1.0 or 50.0 ug. Conversion of {3H}-PGF2α to {3H}-PGE2 or to {3H}-13,14,dihydro-15-keto-PGF2α did not differ in control ovariectomized or ovariectomized rats receiving P or 1.0 ug E0. However, 50.0 ug E0 induced a significant oversion after 30 (P < 0.01) and 60 (P < 0.001) min of incubation. It is concluded that E0, at the 50.0 ug dose, but not the 1.0 ug dose of E0, nor progesterone, stimulated conversion of {3H}-PGE2 into {3H}-PGF2α or {3H}-13, 14,dihydro-15-keto-PGF2α, presumably through the activity of the enzyme PGE2-9-keto-reductase. © 1985.
Registro:
Documento: |
Artículo
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Título: | Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats |
Autor: | Franchi, A.M.; Gimeno, M.F.; Gimeno, A.L. |
Filiación: | Centro de Estudios Farmacológicos y de Principios Naturales (CEFAPRIN), Consejo Nacional de Investigaciones Cientificas y Tecnicas de la Republica Argentina (CONICET). Serr, 1414 Buenos Aires, Argentina
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Palabras clave: | estradiol; progesterone; prostaglandin e2; prostaglandin f2 alpha; radioisotope; animal cell; drug efficacy; endocrine system; female genital system; myometrium; nonhuman; ovariectomy; priority journal; prostaglandin e2 h 3; rat; Animal; Castration; Dinoprost; Dinoprostone; Estradiol; Female; NADP; Progesterone; Prostaglandins E; Prostaglandins F; Rats; Rats, Inbred Strains; Support, Non-U.S. Gov't; Uterus |
Año: | 1985
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Volumen: | 29
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Número: | 5
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Página de inicio: | 773
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Página de fin: | 783
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DOI: |
http://dx.doi.org/10.1016/0090-6980(85)90137-6 |
Título revista: | Prostaglandins
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Título revista abreviado: | Prostaglandins
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ISSN: | 00906980
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CODEN: | PRGLB
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CAS: | estradiol, 50-28-2; progesterone, 57-83-0; prostaglandin E2, 363-24-6; prostaglandin F2 alpha, 551-11-1; 15-keto-13,14-dihydroprostaglandin F2alpha, 27376-76-7; Dinoprost, 551-11-1; Dinoprostone, 363-24-6; Estradiol, 50-28-2; NADP, 53-59-8; Progesterone, 57-83-0; Prostaglandins E; Prostaglandins F
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00906980_v29_n5_p773_Franchi |
Referencias:
- hensby, Distribution studies on the reduction of prostaglandin E2 to prostaglandin F2α by tissue homogenates (1975) Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 409, p. 234
- Lee, Levine, Purification and regulatory properties of chicken heart prostaglandin E 9-keto-reductase (1975) J. Biol. Chem., 250, p. 4549
- Thy, Carpenter, Prostaglandin E2 9-keto-reductase of rat testis (1978) Biochem. Biophys. Res. Commun., 81, p. 322
- Sweatman, Collier, Effects of prostaglandins on human bronchial muscle (1968) Nature, Lond., 217, p. 69
- Dunn, Hood, Prostaglandins and the kidney (1977) Am. J. Physiol., 233, p. 169
- Phariss, Prostaglandins induction of luteolysis (1971) Annals of the New York Academy of Sciences, 180, p. 436
- Marsh, Lemaire, Cyclic AMP accumulation and steroidogenesis in the human corpus luteum. Effect of gonadotrophins and prostaglandins (1974) J. Clin. Endocr. Metab., 38, p. 99
- Channing, Stimulatory effects of prostaglandins upon luteinization of rhesus monkey granulusa cell cultures (1972) Prostaglandins, 2, p. 331
- Horton, Poyser, Uterine luteolytic hormone: a physiological role for prostaglandin F2α (1976) Physiol. Rev., 56, p. 595
- Behrman, Prostaglandins in hypothalamo-pituitary and ovarian function (1979) Ann. Rev. Physiol., 41, p. 685
- McCracken, The role of prostaglandins in luteal regression (1979) Prostaglandins in Fertility Control, pp. 239-258. , S. Bergström, K. Green, B. Samuelsson, W.H.O. Karolinska Institutet, Stockholm
- Watson, Shepherd, Dodson, Prostaglandin E2 9-ketoreductase in ovarian tissues (1979) J. Reprod. Fert., 57, p. 489
- Del Campo, Ginther, Vascular anatomy of the uterus and ovaries and the unilateral luteolytic effect of the uterus: guinea pigs, rats, hamsters and rabbits (1972) Am. J. Vet. Res., 33, p. 2561
- Weems, Prostaglandins F in uterine and ovarian compartments and in plasma from uterine vein, ovarian artery and vein an abdominal aorta of pseudopregnant rats with and without deciduomata (1979) Prostaglandins, 17, p. 873
- Weems, Pexton, Butcher, Inskeep, Prostaglandin F in uterine tissue and venous plasma of pseudopregnant rats: effect of deciduomata (1975) Biol. Reprod., 13, p. 282
- Sterin-Speziale, Gimeno, Bonacossa, Gimeno, The effect of estradiol on isolated rat uterine motility and prostaglandin generation (1980) Prostaglandins, 20, p. 233
- Wong, Terragno, Terragno, McGiff, Dual effects of bradykinin on prostaglandin metabolism: relationship to the dissimilar vascular actions of kinins (1977) Prostaglandins, 13, p. 1113
- Franchi, Chaud, Borda, Gimeno, Lazzari, Gimeno, Spontaneous motility and prostaglandin generation in rat uterine horn isolated during the estrous cycle (1981) Prostaglandins, 22, p. 637
- Ham, Cirillo, Zanetti, Kuehl, Estrogen directed synthesis of specific prostaglandins in uterus (1975) Proc. Nat. Acad. Sci. USA, 72, p. 1420
- Gimeno, Sterin-Speziale, Landa, Bonacossa, Gimeno, Is the spontaneous motility of isolated rat uterus controlled by prostaglandin E? (1979) Prostaglandins, 17, p. 673
- Aizawa, Inazu, Kogo, Catabolism of PGF2α in rat ovary: differences between ovarian and uterine tissues (1980) Prostaglandins, 20, p. 95
- Anggard, Larsson, Samuelsson, The distribution of 15-hydroxy prostaglandin dehydrogenase and prostaglandin Δ13-reductase in tissues of swine (1971) Acta Physiologica Scandinavica, 81, p. 396
- Alam, Russell, Tabor, Moulton, Progesterone and estrogen control of uterine dehydrogenase activity during deciduonal growth (1976) Endocrinology, 98, p. 859
- Blackwell, Flower, Effect of steroid hormones on tissue levels of prostaglandin 15-hydroxy-dehydrogenase in the rat (1976) Br. J. Pharmac., 56, p. 343
- Kogo, Yamada, Aizawa, Effect of estradiol on prostaglandin metabolism in rat uterus (1977) Prostaglandins, 13, p. 785
- Auleta, Agins, Scommegna, Prostaglandin F mediation of the inhibitory effect of estrogen on the corpus luteum of the rhesus monkey (1978) Endocrinology, 103, p. 1183
- Thaler-Dao, Saintot, Ramonatxo, Chavis, Crastes de, Prostaglandin biosynthesis by the rat uterus during the oestrus cycle: Temporal correlation with plasma oestradiol and progesterone concentrations, identification of 6-keto PGF1α as the major metabolite (1982) Prostaglandins, 23, p. 347
Citas:
---------- APA ----------
Franchi, A.M., Gimeno, M.F. & Gimeno, A.L.
(1985)
. Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats. Prostaglandins, 29(5), 773-783.
http://dx.doi.org/10.1016/0090-6980(85)90137-6---------- CHICAGO ----------
Franchi, A.M., Gimeno, M.F., Gimeno, A.L.
"Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats"
. Prostaglandins 29, no. 5
(1985) : 773-783.
http://dx.doi.org/10.1016/0090-6980(85)90137-6---------- MLA ----------
Franchi, A.M., Gimeno, M.F., Gimeno, A.L.
"Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats"
. Prostaglandins, vol. 29, no. 5, 1985, pp. 773-783.
http://dx.doi.org/10.1016/0090-6980(85)90137-6---------- VANCOUVER ----------
Franchi, A.M., Gimeno, M.F., Gimeno, A.L. Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats. Prostaglandins. 1985;29(5):773-783.
http://dx.doi.org/10.1016/0090-6980(85)90137-6