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Abstract:

Formation of {3H}-PGF2α and {3H}-13,14,dihydro-15-keto-PGF2α from {3H}-PGE2 by the supernatant of uterine homogenates from estrous and ovariectomized rats, was studied, using the reaction system PGE2 + NADPH + {3H}-PGE2 + supernatant. Enzymatic conversion was lower in uterine supernatants from spayed rats than in uterine homogenates of rats at natural estrus. Spayed animals were injected with progesterone (P) or with estradiol-17-β (E0) at a dose of 1.0 or 50.0 ug. Conversion of {3H}-PGF2α to {3H}-PGE2 or to {3H}-13,14,dihydro-15-keto-PGF2α did not differ in control ovariectomized or ovariectomized rats receiving P or 1.0 ug E0. However, 50.0 ug E0 induced a significant oversion after 30 (P < 0.01) and 60 (P < 0.001) min of incubation. It is concluded that E0, at the 50.0 ug dose, but not the 1.0 ug dose of E0, nor progesterone, stimulated conversion of {3H}-PGE2 into {3H}-PGF2α or {3H}-13, 14,dihydro-15-keto-PGF2α, presumably through the activity of the enzyme PGE2-9-keto-reductase. © 1985.

Registro:

Documento: Artículo
Título:Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats
Autor:Franchi, A.M.; Gimeno, M.F.; Gimeno, A.L.
Filiación:Centro de Estudios Farmacológicos y de Principios Naturales (CEFAPRIN), Consejo Nacional de Investigaciones Cientificas y Tecnicas de la Republica Argentina (CONICET). Serr, 1414 Buenos Aires, Argentina
Palabras clave:estradiol; progesterone; prostaglandin e2; prostaglandin f2 alpha; radioisotope; animal cell; drug efficacy; endocrine system; female genital system; myometrium; nonhuman; ovariectomy; priority journal; prostaglandin e2 h 3; rat; Animal; Castration; Dinoprost; Dinoprostone; Estradiol; Female; NADP; Progesterone; Prostaglandins E; Prostaglandins F; Rats; Rats, Inbred Strains; Support, Non-U.S. Gov't; Uterus
Año:1985
Volumen:29
Número:5
Página de inicio:773
Página de fin:783
DOI: http://dx.doi.org/10.1016/0090-6980(85)90137-6
Título revista:Prostaglandins
Título revista abreviado:Prostaglandins
ISSN:00906980
CODEN:PRGLB
CAS:estradiol, 50-28-2; progesterone, 57-83-0; prostaglandin E2, 363-24-6; prostaglandin F2 alpha, 551-11-1; 15-keto-13,14-dihydroprostaglandin F2alpha, 27376-76-7; Dinoprost, 551-11-1; Dinoprostone, 363-24-6; Estradiol, 50-28-2; NADP, 53-59-8; Progesterone, 57-83-0; Prostaglandins E; Prostaglandins F
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00906980_v29_n5_p773_Franchi

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Citas:

---------- APA ----------
Franchi, A.M., Gimeno, M.F. & Gimeno, A.L. (1985) . Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats. Prostaglandins, 29(5), 773-783.
http://dx.doi.org/10.1016/0090-6980(85)90137-6
---------- CHICAGO ----------
Franchi, A.M., Gimeno, M.F., Gimeno, A.L. "Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats" . Prostaglandins 29, no. 5 (1985) : 773-783.
http://dx.doi.org/10.1016/0090-6980(85)90137-6
---------- MLA ----------
Franchi, A.M., Gimeno, M.F., Gimeno, A.L. "Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats" . Prostaglandins, vol. 29, no. 5, 1985, pp. 773-783.
http://dx.doi.org/10.1016/0090-6980(85)90137-6
---------- VANCOUVER ----------
Franchi, A.M., Gimeno, M.F., Gimeno, A.L. Estradiol -17-β enhances the formation of {3H}-PGF2α from {3H}-PGE2 in the uterus isolated from ovariectomized rats. Prostaglandins. 1985;29(5):773-783.
http://dx.doi.org/10.1016/0090-6980(85)90137-6