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Abstract:

Incubated bovine pineal glands released prostaglandin E- and prostaglandin F-like material (304 ± 20 and 582 ± 56 pg/mg dry tissue wt/h, respectively) and the release was increased 2.2 2.9-fold by adding 10-4-10-6M of norepinephrine to the medium. Binding assays revealed the existence of high affinity binding of 3H-prostaglandin E2 (3H-PGE2) and 3H-prostaglandin F2α (3H-PGF2α) in low speed supernatants of pineal homogenates. Binding was increased by increasing Ca++ concentration in medium up to 2 mM, was heat-labile and was depressed following incubation with trypsin. In subcellular fractionation studies maximal 3H-PG binding was found in the 27000 × g pellet. Scatchard analysis of 3H-PGE2 binding revealed the presence of a single population of binding sites with a Kd= 1.2 nM and a binding site concentration of 1-2 pmoles/g protein. A single population of binding sites for 3H-PGF2α was also detected with a Kd= 1.7 nM and a similar binding site concentration. Non-radioactive PGE1 and PGE2 were almost equally effective to compete for 3H-PGE2 binding sites (ED50= 5 and 2 nM, respectively). Unlabeled PGF2 was relatively ineffective to compete for 3H-PGE2 binding (ED50>1000 nM) but displaced effectively 3H-PGF2α binding (ED50= 1.2 nM). © 1979.

Registro:

Documento: Artículo
Título:Release and specific binding of prostaglandins in bovine pineal gland
Autor:Cardinali, D.P.; Ritta, M.N.; Speziale, N.S.; Gimeno, M.F.
Filiación:Centro de Estudios Farmacológicos y de Principios Naturales (CEFAPRIN), Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Norepinephrine; Pineal Gland; Prostaglandin High Affinity Binding; Prostaglandin Receptors; Sympathetic Nervous System; indometacin; noradrenalin; prostaglandin; prostaglandin e1; prostaglandin e2; radioisotope; animal experiment; cattle; drug binding; drug release; endocrine system; in vitro study; pineal body; prostaglandin e2 h 3; prostaglandin f2 alpha h 3; radioimmunoassay; Animal; Cattle; Culture Media; Norepinephrine; Pineal Gland; Prostaglandins E; Prostaglandins F; Receptors, Prostaglandin; Secretory Rate
Año:1979
Volumen:18
Número:4
Página de inicio:577
Página de fin:589
DOI: http://dx.doi.org/10.1016/0090-6980(79)90025-X
Título revista:Prostaglandins
Título revista abreviado:Prostaglandins
ISSN:00906980
CODEN:PRGLB
CAS:indometacin, 53-86-1, 74252-25-8, 7681-54-1; noradrenalin, 1407-84-7, 51-41-2; prostaglandin E1, 745-65-3; prostaglandin E2, 363-24-6; Culture Media; Norepinephrine, 51-41-2; Prostaglandins E; Prostaglandins F; Receptors, Prostaglandin
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00906980_v18_n4_p577_Cardinali

Referencias:

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Citas:

---------- APA ----------
Cardinali, D.P., Ritta, M.N., Speziale, N.S. & Gimeno, M.F. (1979) . Release and specific binding of prostaglandins in bovine pineal gland. Prostaglandins, 18(4), 577-589.
http://dx.doi.org/10.1016/0090-6980(79)90025-X
---------- CHICAGO ----------
Cardinali, D.P., Ritta, M.N., Speziale, N.S., Gimeno, M.F. "Release and specific binding of prostaglandins in bovine pineal gland" . Prostaglandins 18, no. 4 (1979) : 577-589.
http://dx.doi.org/10.1016/0090-6980(79)90025-X
---------- MLA ----------
Cardinali, D.P., Ritta, M.N., Speziale, N.S., Gimeno, M.F. "Release and specific binding of prostaglandins in bovine pineal gland" . Prostaglandins, vol. 18, no. 4, 1979, pp. 577-589.
http://dx.doi.org/10.1016/0090-6980(79)90025-X
---------- VANCOUVER ----------
Cardinali, D.P., Ritta, M.N., Speziale, N.S., Gimeno, M.F. Release and specific binding of prostaglandins in bovine pineal gland. Prostaglandins. 1979;18(4):577-589.
http://dx.doi.org/10.1016/0090-6980(79)90025-X