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Abstract:

The extracellular production of ligninolytic enzymes by xylariaceous and diatrypaceous species from Argentina was investigated. Among them, a new species, Eutypa microasca, was evaluated. Its morphological characteristics (e.g.: very small asci) along with the molecular analyses, support the hypothesis of the new taxon. Ligninolytic enzyme production by several saprotrophic and endophytic strains belonging to the genera Xylaria, Eutypella, Eutypa and Peroneutypa was assessed on solid medium supplemented with different dyes (Poly R-478, Azure B and Malachite Green) and under submerged fermentation in copper amended malt extract/glucose medium. All the strains produced lacease in liquid cultures, and seven out of nine also produced Mn-peroxidase (highest titres detected 113 U/l). Only five of the strains assayed could not decolorize Malachite Green. This is the first report on laccase and Mn-peroxidase production by Diatrypaceae and contributes to the knowledge on ligninolytic enzyme production in Xylariaceae.

Registro:

Documento: Artículo
Título:Studies in diatrypaceae: The new species Eutypa microasca and investigation of ligninolytic enzyme production
Autor:Grassi, E.; Pildain, M.B.; Levin, L.; Carmaran, C.
Filiación:Departamento de Biodiversidad Y Biología Experimental, Facultad de Cs. Exactas Y Naturales (UBA), Ciudad Universitaria, Pabello and Diacriticaĺn II, 4to Piso, C1428EHA, Buenos Aires, Argentina
ÁRea de Protección Forestal, Centro de Investigación Y Extensión Forestal Andino Patagónico, C. C. 14, 9200 Esquel, Chubut, Argentina
Palabras clave:Enzyme assay; Eutypella; Lacease; Peroneutypa; Xylaria.
Año:2014
Volumen:66
Número:1
Página de inicio:99
Página de fin:114
DOI: http://dx.doi.org/10.12905/0380.sydowia66(1)2014-0099
Título revista:Sydowia
Título revista abreviado:Sydowia
ISSN:00820598
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00820598_v66_n1_p99_Grassi

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Citas:

---------- APA ----------
Grassi, E., Pildain, M.B., Levin, L. & Carmaran, C. (2014) . Studies in diatrypaceae: The new species Eutypa microasca and investigation of ligninolytic enzyme production. Sydowia, 66(1), 99-114.
http://dx.doi.org/10.12905/0380.sydowia66(1)2014-0099
---------- CHICAGO ----------
Grassi, E., Pildain, M.B., Levin, L., Carmaran, C. "Studies in diatrypaceae: The new species Eutypa microasca and investigation of ligninolytic enzyme production" . Sydowia 66, no. 1 (2014) : 99-114.
http://dx.doi.org/10.12905/0380.sydowia66(1)2014-0099
---------- MLA ----------
Grassi, E., Pildain, M.B., Levin, L., Carmaran, C. "Studies in diatrypaceae: The new species Eutypa microasca and investigation of ligninolytic enzyme production" . Sydowia, vol. 66, no. 1, 2014, pp. 99-114.
http://dx.doi.org/10.12905/0380.sydowia66(1)2014-0099
---------- VANCOUVER ----------
Grassi, E., Pildain, M.B., Levin, L., Carmaran, C. Studies in diatrypaceae: The new species Eutypa microasca and investigation of ligninolytic enzyme production. Sydowia. 2014;66(1):99-114.
http://dx.doi.org/10.12905/0380.sydowia66(1)2014-0099