Abstract:
In this work we describe a flow cytometry-based method using SYTO16 (a DNA intercalating agent) to quantify Anaplasma marginale-infected erythrocytes in blood from bovine animals. The linearity and reproducibility of the results obtained with SYTO16 labeling followed by flow cytometry analysis make it a suitable approach for measurement of parasitemia in A. marginale infections. © 2008 New York Academy of Sciences.
Registro:
Documento: |
Artículo
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Título: | Flow cytometry to evaluate Anaplasma marginale parasitemia using a fluorescent nucleic acid stain |
Autor: | Moretta, R.; Ruybal, P.; Mesplet, M.; Petrigh, R.; Nuñez, P.; Gil, G.; Wilkowsky, S.; Garbossa, G.; Farber, M. |
Filiación: | CICVyA-INTA Castelar, Buenos Aires, Argentina Facultad de Ciencias Exactas Y Naturales, UBA, Buenos Aires, Argentina Instituto de Biotecnología, CICVyA, INTA, Los Reseros Y Las Cabañas, CP 1712, Castelar, Buenos Aires, Argentina
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Palabras clave: | Anaplasma marginale; Flow cytometry; Parasitemia; SYTO16; Anaplasma marginale; animal cell; cattle; conference paper; controlled study; erythrocyte; flow cytometry; measurement; nonhuman; parasite examination; parasitemia; reproducibility; Anaplasma marginale; Animalia; Bovinae |
Año: | 2008
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Volumen: | 1149
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Página de inicio: | 111
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Página de fin: | 113
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DOI: |
http://dx.doi.org/10.1196/annals.1428.039 |
Título revista: | Annals of the New York Academy of Sciences
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Título revista abreviado: | Ann. New York Acad. Sci.
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ISSN: | 00778923
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CODEN: | ANYAA
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00778923_v1149_n_p111_Moretta |
Referencias:
- Kocan, K.M., Anaplasmosis control: Past, present, and future (2000) Ann. N. Y. Acad. Sci., 916, pp. 501-509
- Coetzee, J.F., Flow cytometric evaluation of selected antimicrobial efficacy for clearance of Anaplasma marginale in short-term erythrocyte cultures (2006) J. Vet. Pharmacol. Ther., 29, pp. 173-183
- Yagi, Y., Flow cytometry to evaluate Theileria sergenti parasitemia using the fluorescent nucleic acid stain, SYTO16 (2000) Cytometry, 41, pp. 223-225
Citas:
---------- APA ----------
Moretta, R., Ruybal, P., Mesplet, M., Petrigh, R., Nuñez, P., Gil, G., Wilkowsky, S.,..., Farber, M.
(2008)
. Flow cytometry to evaluate Anaplasma marginale parasitemia using a fluorescent nucleic acid stain. Annals of the New York Academy of Sciences, 1149, 111-113.
http://dx.doi.org/10.1196/annals.1428.039---------- CHICAGO ----------
Moretta, R., Ruybal, P., Mesplet, M., Petrigh, R., Nuñez, P., Gil, G., et al.
"Flow cytometry to evaluate Anaplasma marginale parasitemia using a fluorescent nucleic acid stain"
. Annals of the New York Academy of Sciences 1149
(2008) : 111-113.
http://dx.doi.org/10.1196/annals.1428.039---------- MLA ----------
Moretta, R., Ruybal, P., Mesplet, M., Petrigh, R., Nuñez, P., Gil, G., et al.
"Flow cytometry to evaluate Anaplasma marginale parasitemia using a fluorescent nucleic acid stain"
. Annals of the New York Academy of Sciences, vol. 1149, 2008, pp. 111-113.
http://dx.doi.org/10.1196/annals.1428.039---------- VANCOUVER ----------
Moretta, R., Ruybal, P., Mesplet, M., Petrigh, R., Nuñez, P., Gil, G., et al. Flow cytometry to evaluate Anaplasma marginale parasitemia using a fluorescent nucleic acid stain. Ann. New York Acad. Sci. 2008;1149:111-113.
http://dx.doi.org/10.1196/annals.1428.039