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Abstract:

In mammals, glucocorticoids (GC) are inactivated by the oxidative activity of 11β-hydroxysteroid dehydrogenase (11β-HSD). To study that mechanism in the testes of Bufo arenarum, 11β-HSD activity and plasma corticosterone (B) were determined in toads from pre- (PR), post- (P), and reproductive (R) periods. Toad 11β-HSD is NAD+-dependent and strongly inhibited by glycyrrhetinic acid. Vmax is higher in the P period (1.37 vs. R: 0.13 and PR: 0.26 pmol/min-mg protein), whereas Km values (around 200 nM) remain constant. Plasma B concentrations (R: 178; PR: 61; P: 114 nM) are close to Km values, suggesting that, under baseline conditions, 11β-HSD could protect testes against GC action. © 2005 New York Academy of Sciences.

Registro:

Documento: Artículo
Título:Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes
Autor:Denari, D.; Ceballos, N.R.
Filiación:Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Biodiversidad y Biologia Experimental, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
Palabras clave:11β-hydroxysteroid dehydrogenase; Corticosterone; Testis; Toad; 11beta hydroxysteroid dehydrogenase; corticosterone; glucocorticoid; glucose 6 phosphatase; glycyrrhetinic acid; nicotinamide adenine dinucleotide; succinate dehydrogenase; animal experiment; animal tissue; cell nucleus; cellular distribution; conference paper; controlled study; corticosterone blood level; cytosol; enzyme activity; Leydig cell; male; Michaelis constant; microsome; mitochondrion; nonhuman; oxidation; reproduction; seasonal variation; testis; toad; Anura; Bufo arenarum; Mammalia
Año:2005
Volumen:1040
Página de inicio:297
Página de fin:300
DOI: http://dx.doi.org/10.1196/annals.1327.046
Título revista:Annals of the New York Academy of Sciences
Título revista abreviado:Ann. New York Acad. Sci.
ISSN:00778923
CODEN:ANYAA
CAS:11beta hydroxysteroid dehydrogenase, 9041-46-7; corticosterone, 50-22-6; glucose 6 phosphatase, 9001-39-2; glycyrrhetinic acid, 471-53-4; nicotinamide adenine dinucleotide, 53-84-9; succinate dehydrogenase, 9002-02-2, 9028-10-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00778923_v1040_n_p297_Denari

Referencias:

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Citas:

---------- APA ----------
Denari, D. & Ceballos, N.R. (2005) . Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes. Annals of the New York Academy of Sciences, 1040, 297-300.
http://dx.doi.org/10.1196/annals.1327.046
---------- CHICAGO ----------
Denari, D., Ceballos, N.R. "Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes" . Annals of the New York Academy of Sciences 1040 (2005) : 297-300.
http://dx.doi.org/10.1196/annals.1327.046
---------- MLA ----------
Denari, D., Ceballos, N.R. "Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes" . Annals of the New York Academy of Sciences, vol. 1040, 2005, pp. 297-300.
http://dx.doi.org/10.1196/annals.1327.046
---------- VANCOUVER ----------
Denari, D., Ceballos, N.R. Seasonal changes in the activity of 11β-hydroxysteroid dehydrogenase in toad testes. Ann. New York Acad. Sci. 2005;1040:297-300.
http://dx.doi.org/10.1196/annals.1327.046