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In the postgenomic era, the study of the glycome-the whole repertoire of saccharides in cells and tissues-has enabled the association of unique glycan structures with specific physiological and pathological processes. The responsibility for deciphering this biological information belongs to endogenous glycan-binding proteins or lectins. Galectin-1, a prototypic member of a family of structurally related proteins, has demonstrated selective antiinflammatory and immunoregulatory effects either by controlling immune cell trafficking, "fine-tuning" dendritic cell physiology and regulating T-cell fate. These regulatory functions mediated by an endogenous glycan-binding protein may contribute to fulfill the needs for immune cell homeostasis, including preservation of fetomaternal tolerance and prevention of collateral damage as a result of microbial invasion or autoimmune pathology. We will discuss here the conceptual framework which led to the study of galectin-glycan lattices as a novel paradigm of immune cell communication in physiological and pathological processes and will highlight selected methods and experimental strategies which have contributed to the study of the immunoregulatory activities of this multifaceted glycan-binding protein both in in vitro and in vivo biological settings. © 2010 Elsevier Inc.


Documento: Artículo
Título:Multiple functional targets of the immunoregulatory activity of Galectin-1. Control of immune cell trafficking, dendritic cell physiology, and T-cell fate
Autor:Cooper, D.; Ilarregui, J.M.; Pesoa, S.A.; Croci, D.O.; Perretti, M.; Rabinovich, G.A.
Filiación:The William Harvey Research Institute, Barts and The London School of Medicine, Queen Mary University of London, London, United Kingdom
Laboratorio de Inmunopatología, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Dendritic cells; Galectin-1; Glycoimmunology; Homeostasis; Inflammation; Neutrophils; T cells; Tolerance; Trafficking; galectin 1; adoptive transfer; antiinflammatory activity; article; autoimmune disease; binding assay; carbohydrate analysis; cell count; cell culture; cell death; cell differentiation; cell fate; cell function; cell isolation; cell maturation; cell migration; cell polarity; cell surface; cell survival; chemotaxis; conceptual framework; cremaster muscle; dendritic cell; disease model; endocytosis; endothelium cell; helper cell; homeostasis; human; immunological tolerance; immunoregulation; leukocyte; mesentery; microvasculature; mother fetus relationship; nonhuman; peritonitis; phenotype; priority journal; protein function; quantitative analysis; real time polymerase chain reaction; regulatory mechanism; T lymphocyte; Th1 cell; Th17 cell; Th2 cell; Western blotting; Animals; Chemotaxis, Leukocyte; Dendritic Cells; Galectin 1; Humans; Immune System; Immunomodulation; Laboratory Techniques and Procedures; Models, Biological; T-Lymphocytes
Página de inicio:199
Página de fin:244
Título revista:Methods in Enzymology
Título revista abreviado:Methods Enzymol.
CAS:galectin 1, 258495-34-0; Galectin 1


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---------- APA ----------
Cooper, D., Ilarregui, J.M., Pesoa, S.A., Croci, D.O., Perretti, M. & Rabinovich, G.A. (2010) . Multiple functional targets of the immunoregulatory activity of Galectin-1. Control of immune cell trafficking, dendritic cell physiology, and T-cell fate. Methods in Enzymology, 480(C), 199-244.
---------- CHICAGO ----------
Cooper, D., Ilarregui, J.M., Pesoa, S.A., Croci, D.O., Perretti, M., Rabinovich, G.A. "Multiple functional targets of the immunoregulatory activity of Galectin-1. Control of immune cell trafficking, dendritic cell physiology, and T-cell fate" . Methods in Enzymology 480, no. C (2010) : 199-244.
---------- MLA ----------
Cooper, D., Ilarregui, J.M., Pesoa, S.A., Croci, D.O., Perretti, M., Rabinovich, G.A. "Multiple functional targets of the immunoregulatory activity of Galectin-1. Control of immune cell trafficking, dendritic cell physiology, and T-cell fate" . Methods in Enzymology, vol. 480, no. C, 2010, pp. 199-244.
---------- VANCOUVER ----------
Cooper, D., Ilarregui, J.M., Pesoa, S.A., Croci, D.O., Perretti, M., Rabinovich, G.A. Multiple functional targets of the immunoregulatory activity of Galectin-1. Control of immune cell trafficking, dendritic cell physiology, and T-cell fate. Methods Enzymol. 2010;480(C):199-244.