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Abstract:

In the postgenomic era, the study of the glycome-the whole repertoire of saccharides in cells and tissues-has enabled the association of unique glycan structures with specific physiological and pathological processes. The responsibility for deciphering this biological information belongs to endogenous glycan-binding proteins or lectins. Galectin-1, a prototypic member of a family of structurally related proteins, has demonstrated selective antiinflammatory and immunoregulatory effects either by controlling immune cell trafficking, "fine-tuning" dendritic cell physiology and regulating T-cell fate. These regulatory functions mediated by an endogenous glycan-binding protein may contribute to fulfill the needs for immune cell homeostasis, including preservation of fetomaternal tolerance and prevention of collateral damage as a result of microbial invasion or autoimmune pathology. We will discuss here the conceptual framework which led to the study of galectin-glycan lattices as a novel paradigm of immune cell communication in physiological and pathological processes and will highlight selected methods and experimental strategies which have contributed to the study of the immunoregulatory activities of this multifaceted glycan-binding protein both in in vitro and in vivo biological settings. © 2010 Elsevier Inc.

Registro:

Documento: Artículo
Título:Multiple functional targets of the immunoregulatory activity of Galectin-1. Control of immune cell trafficking, dendritic cell physiology, and T-cell fate
Autor:Cooper, D.; Ilarregui, J.M.; Pesoa, S.A.; Croci, D.O.; Perretti, M.; Rabinovich, G.A.
Filiación:The William Harvey Research Institute, Barts and The London School of Medicine, Queen Mary University of London, London, United Kingdom
Laboratorio de Inmunopatología, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Dendritic cells; Galectin-1; Glycoimmunology; Homeostasis; Inflammation; Neutrophils; T cells; Tolerance; Trafficking; galectin 1; adoptive transfer; antiinflammatory activity; article; autoimmune disease; binding assay; carbohydrate analysis; cell count; cell culture; cell death; cell differentiation; cell fate; cell function; cell isolation; cell maturation; cell migration; cell polarity; cell surface; cell survival; chemotaxis; conceptual framework; cremaster muscle; dendritic cell; disease model; endocytosis; endothelium cell; helper cell; homeostasis; human; immunological tolerance; immunoregulation; leukocyte; mesentery; microvasculature; mother fetus relationship; nonhuman; peritonitis; phenotype; priority journal; protein function; quantitative analysis; real time polymerase chain reaction; regulatory mechanism; T lymphocyte; Th1 cell; Th17 cell; Th2 cell; Western blotting; Animals; Chemotaxis, Leukocyte; Dendritic Cells; Galectin 1; Humans; Immune System; Immunomodulation; Laboratory Techniques and Procedures; Models, Biological; T-Lymphocytes
Año:2010
Volumen:480
Número:C
Página de inicio:199
Página de fin:244
DOI: http://dx.doi.org/10.1016/S0076-6879(10)80011-4
Título revista:Methods in Enzymology
Título revista abreviado:Methods Enzymol.
ISSN:00766879
CODEN:MENZA
CAS:galectin 1, 258495-34-0; Galectin 1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00766879_v480_nC_p199_Cooper

Referencias:

  • Ajuebor, M.N., Gibbs, L., Flower, R.J., Das, A.M., Perretti, M., Investigation of the functional role played by the chemokine monocyte chemoattractant protein-1 in interleukin-1-induced murine peritonitis (1998) Br. J. Pharmacol., 125, pp. 319-326
  • Ajuebor, M.N., Flower, R.J., Hannon, R., Christie, M., Bowers, K., Verity, A., Perretti, M., Endogenous monocyte chemoattractant protein-1 recruits monocytes in the zymosan peritonitis model (1998) J. Leukoc. Biol., 63, pp. 108-116
  • Amano, M., Galvan, M., Baum, L.G., The ST6Gal I sialyltransferase selectively modifies N-glycans on CD45 to negatively regulate galectin-1-induced CD45 clustering, phosphatase modulation, and T cell death (2003) J. Biol. Chem., 278, pp. 7469-7475
  • Barrionuevo, P., Beigier-Bompadre, M., Ilarregui, J.M., Toscano, M.A., Bianco, G.A., Isturiz, M.A., Rabinovich, G.A., A novel function for galectin-1 at the crossroad of innate and adaptive immunity: Galectin-1 regulates monocyte/macrophage physiology through a nonapoptotic ERK-dependent pathway (2007) J. Immunol., 178, pp. 436-445
  • Bettelli, E., Carrier, Y., Gao, W., Korn, T., Strom, T.B., Oukka, M., Weiner, H.L., Kuchroo, V.K., Reciprocal developmental pathways for the generation of pathogenic effector TH-17 and regulatory T cells (2006) Nature, 441, pp. 235-238
  • Bidère, N., Su, H.C., Lenardo, M.J., Genetic disorders of programmed cell death in the immune system (2006) Annu. Rev. Immunol., 24, pp. 321-352
  • Bienvenu, K., Granger, D.N., Molecular determinants of shear rate-dependent leukocyte adhesion in postcapillary venules (1993) Am. J. Physiol., 264, pp. H1504-H1508
  • Blaser, C., Kaufmann, M., Müller, C., Zimmermann, C., Wells, V., Mallucci, L., Pircher, H., Beta-galactoside-binding protein secreted by activated T cells inhibits antigen-induced proliferation of T cells (1998) Eur. J. Immunol., 28, pp. 2311-2319
  • Blois, S.M., Ilarregui, J.M., Tometten, M., Garcia, M., Orsal, A.S., Cordo-Russo, R., Toscano, M.A., Markert, U.R., A pivotal role for galectin-1 in fetomaternal tolerance (2007) Nat. Med., 13, pp. 1450-1457
  • Bluestone, J.A., Thomson, A.W., Shevach, E.M., Weiner, H.L., What does the future hold for cell-based tolerogenic therapy? (2007) Nat. Rev. Immunol., 7, pp. 650-654
  • Brewer, C.F., Miceli, M.C., Baum, L.G., Clusters, bundles, arrays and lattices: Novel mechanisms for lectin-saccharide-mediated cellular interactions (2002) Curr. Opin. Struct. Biol., 12, pp. 616-623
  • Cabrera, P.V., Amano, M., Mitoma, J., Chan, J., Said, J., Fukuda, M., Baum, L.G., Haploinsufficiency of C2GnT-I glycosyltransferase renders T lymphoma cells resistant to cell death (2006) Blood, 108, pp. 2399-2406
  • Cervi, L., MacDonald, A.S., Kane, C., Dzierszinski, F., Pearce, E.J., Cutting edge: Dendritic cells copulsed with microbial and helminth antigens undergo modified maturation, segregate the antigens to distinct intracellular compartments, and concurrently induce microbe-specific Th1 and helminth-specific Th2 responses (2004) J. Immunol., 172, pp. 2016-2020
  • Cooper, D., Norling, L.V., Perretti, M., Novel insights into the inhibitory effects of Galectin-1 on neutrophil recruitment under flow (2008) J. Leukoc. Biol., 83, pp. 1459-1466
  • Cummings, R.D., Liu, F.T., Galectins (2008) Essentials of Glycobiology, pp. 475-487. , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, A. Varki (Ed.)
  • Dahlgren, C., Christophe, T., Boulay, F., Madianos, P.N., Rabiet, M.J., Karlsson, A., The synthetic chemoattractant Trp-Lys-Tyr-Met-Val-DMet activates neutrophils preferentially through the lipoxin A(4) receptor (2000) Blood, 95, pp. 1810-1818
  • Dettin, L., Rubinstein, N., Aoki, A., Rabinovich, G.A., Maldonado, C.A., Regulated expression and ultrastructural localization of galectin-1, a proapoptotic beta-galactoside-binding lectin, during spermatogenesis in rat testis (2003) Biol. Reprod., 68, pp. 51-59
  • Dias-Baruffi, M., Zhu, H., Cho, M., Karmakar, S., McEver, R.P., Cummings, R.D., Dimeric galectin-1 induces surface exposure of phosphatidylserine and phagocytic recognition of leukocytes without inducing apoptosis (2003) J. Biol. Chem., 278, pp. 41282-41293
  • Earl, L.A., Bi, S., Baum, L.G., N- and O-glycans modulate galectin-1 binding, CD45 signaling, and T cell death (2010) J. Biol. Chem., 285, pp. 2232-2244
  • Fife, B.T., Bluestone, J.A., Control of peripheral T-cell tolerance and autoimmunity via the CTLA-4 and PD-1 pathways (2008) Immunol. Rev., 224, pp. 166-182
  • Fitch, F.W., Gajewski, T.F., Hu-Li, J., Production of Th1 and Th2 cell lines and clones (2008) Current Protocols in Immunology, pp. 3.13.1-3.13.15. , John Wiley & Sons, Inc, New York, J.E. Coligan (Ed.)
  • Fuertes, M.B., Molinero, L.L., Toscano, M.A., Ilarregui, J.M., Rubinstein, N., Fainboim, L., Zwirner, N.W., Rabinovich, G.A., Regulated expression of galectin-1 during T-cell activation involves Lck and Fyn kinases and signaling through MEK1/ERK, p38 MAP kinase and p70S6 kinase (2004) Mol. Cell. Biochem., 267, pp. 177-185
  • Fulcher, J.A., Chang, M.H., Wang, S., Almazan, T., Hashimi, S.T., Eriksson, A.U., Wen, X., Lee, B., Galectin-1 co-clusters CD43/CD45 on dendritic cells and induces cell activation and migration through Syk and protein kinase C signaling (2009) J. Biol. Chem., 284, pp. 26860-26870
  • Galluzzi, L., Aaronson, S.A., Abrams, J., Alnemri, E.S., Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes (2009) Cell Death Differ., 16, pp. 1093-1107
  • Garín, M.I., Chu, C.C., Golshayan, D., Cernuda-Morollón, E., Wait, R., Lechler, R.I., Galectin-1: A key effector of regulation mediated by CD4+CD25+ T cells (2007) Blood, 109, pp. 2058-2065
  • Gil, C.D., Cooper, D., Rosignoli, G., Perretti, M., Oliani, S.M., Inflammation-induced modulation of cellular galectin-1 and -3 expression in a model of rat peritonitis (2006) Inflamm. Res., 55, pp. 99-107
  • Hannier, S., Bitegye, C., Demotz, S., Early events of TCR signaling are distinct in human Th1 and Th2 cells (2002) J. Immunol., 169, pp. 1904-1911
  • Harjacek, M., Diaz-Cano, S., De Miguel, M., Wolfe, H., Maldonado, C.A., Rabinovich, G.A., Expression of galectins-1 and -3 correlates with defective mononuclear cell apoptosis in patients with juvenile idiopathic arthritis (2001) J. Rheumatol., 28, pp. 1914-1922
  • Hernandez, J.D., Nguyen, J.T., He, J., Wang, W., Ardman, B., Green, J.M., Fukuda, M., Baum, L.G., Galectin-1 binds different CD43 glycoforms to cluster CD43 and regulate T cell death (2006) J.Immunol, 177, pp. 5328-5336
  • Hsu, K.L., Mahal, L.K., Sweet tasting chips: Microarray-based analysis of glycans (2009) Curr. Opin. Chem. Biol., 13, pp. 427-432
  • Iglesias, M.M., Rabinovich, G.A., Ivanovic, V., Sotomayor, C., Wolfenstein-Todel, C., Galectin-1 from ovine placenta-amino-acid sequence, physicochemical properties and implications in T-cell death (1998) Eur. J. Biochem., 252, pp. 400-407
  • Ilarregui, J.M., Rabinovich, G.A., Tolerogenic dendritic cells in the control of autoimmune neuroinflammation: An emerging role of protein-glycan interactions (2010) Neuroimmunomodulation, 17, pp. 157-160
  • Ilarregui, J.M., Croci, D.O., Bianco, G.A., Toscano, M.A., Salatino, M., Vermeulen, M.E., Geffner, J.R., Rabinovich, G.A., Tolerogenic signals delivered by dendritic cells to T cells through a galectin-1-driven immunoregulatory circuit involving interleukin 27 and interleukin 10 (2009) Nat. Immunol., 10, pp. 981-991
  • Inaba, K., Inaba, M., Romani, N., Aya, H., Deguchi, M., Ikehara, S., Muramatsu, S., Steinman, R.M., Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor (1992) J. Exp. Med., 176, pp. 1693-1702
  • Ishida, K., Panjwani, N., Cao, Z., Streilein, J.W., Participation of pigment epithelium in ocular immune privilege. 3. Epithelia cultured from iris, ciliary body, and retina suppress T-cell activation by partially non-overlapping mechanisms (2003) Ocul. Immunol. Inflamm., 11, pp. 91-105
  • Juszczynski, P., Ouyang, J., Monti, S., Rodig, S.J., Takeyama, K., Abramson, J., Chen, W., Shipp, M.A., The AP1-dependent secretion of galectin-1 by Reed Sternberg cells fosters immune privilege in classical Hodgkin lymphoma (2007) Proc. Natl. Acad. Sci. USA, 104, pp. 13134-13139
  • Kopcow, H.D., Rosetti, F., Leung, Y., Allan, D.S., Kutok, J.L., Strominger, J.L., T cell apoptosis at the maternal-fetal interface in early human pregnancy, involvement of galectin-1 (2008) Proc. Natl. Acad. Sci. USA, 105, pp. 18472-18477
  • La, M., Cao, T.V., Cerchiaro, G., Chilton, K., Hirabayashi, J., Kasai, K., Oliani, S.M., Perretti, M., A novel biological activity for galectin-1: Inhibition of leukocyte-endothelial cell interactions in experimental inflammation (2003) Am. J. Pathol., 163, pp. 1505-1515
  • Levi, G., Teichberg, V.I., Isolation and physicochemical characterization of electrolectin, a beta-d-galactoside binding lectin from the electric organ of Electrophorus electricus (1981) J. Biol. Chem., 256, pp. 5735-5740
  • Liu, F.T., Rabinovich, G.A., Galectins as modulators of tumour progression (2005) Nat. Rev. Cancer, 5, pp. 29-41
  • Lopez-Diego, R.S., Weiner, H.L., Novel therapeutic strategies for multiple sclerosis -a multifaceted adversary (2008) Nat. Rev. Drug Discov., 7, pp. 909-925
  • Mangan, P.R., Harrington, L.E., O'Quinn, D.B., Helms, W.S., Bullard, D.C., Elson, C.O., Hatton, R.D., Weaver, C.T., Transforming growth factor-b induces development of the TH-17 lineage (2006) Nature, 441, pp. 231-234
  • Maynard, C.L., Weaver, C.T., Diversity in the contribution of interleukin-10 to T-cell-mediated immune regulation (2008) Immunol. Rev., 226, pp. 219-233
  • Morgan, R., Gao, G., Pawling, J., Dennis, J.W., Demetriou, M., Li, B., N-acetylglucosaminyltransferase V (Mgat5)-mediated N-glycosylation negatively regulates Th1 cytokine production by T cells (2004) J. Immunol., 173, pp. 7200-7208
  • Motran, C.C., Molinder, K.M., Liu, S.D., Poirier, F., Miceli, M.C., Galectin-1 functions as a Th2 cytokine that selectively induces Th1 apoptosis and promotes Th2 function (2008) Eur. J. Immunol., 38, pp. 3015-3027
  • Norling, L.V., Sampaio, A.L., Cooper, D., Perretti, M., Inhibitory control of endothelial galectin-1 on in vitro and in vivo lymphocyte trafficking (2008) FASEB J., 22, pp. 682-690
  • Pace, K.E., Lee, C., Stewart, P.L., Baum, L.G., Restricted receptor segregation into membrane microdomains occurs on human T cells during apoptosis induced by galectin-1 (1999) J. Immunol., 163, pp. 3801-3811
  • Pace, K.E., Hahn, H.P., Baum, L.G., Preparation of recombinant human galectin-1 and use in T-cell death assays (2003) Methods Enzymol., 363, pp. 499-518
  • Pacienza, N., Pozner, R.G., Bianco, G.A., D'Atri, L.P., Croci, D.O., Negrotto, S., Malaver, E., Schattner, M., The immunoregulatory glycan-binding protein galectin-1 triggers human platelet activation (2008) FASEB J., 22, pp. 1113-1123
  • Peggs, K.S., Quezada, S.A., Allison, J.P., Cell intrinsic mechanisms of T-cell inhibition and application to cancer therapy (2008) Immunol. Rev., 224, pp. 141-165
  • Perillo, N.L., Uittenbogaart, C.H., Nguyen, J.T., Baum, L.G., Galectin-1, an endogenous lectin produced by thymic epithelial cells, induces apoptosis of human thymocytes (1997) J. Exp. Med., 185, pp. 1851-1858
  • Perone, M.J., Larregina, A.T., Shufesky, W.J., Papworth, G.D., Sullivan, M.L., Zahorchak, A.F., Stolz, D.B., Morelli, A.E., Transgenic galectin-1 induces maturation of dendritic cells that elicit contrasting responses in naive and activated T cells (2006) J. Immunol., 176, pp. 7207-7220
  • Poirier, F., Robertson, E.J., Normal development of mice carrying a null mutation in the gene encoding the L14 S-type lectin (1993) Development, 119, pp. 1229-1236
  • Rabinovich, G.A., Galectin-1 as a potential cancer target (2005) Br. J. Cancer, 92, pp. 1188-1192
  • Rabinovich, G.A., Gruppi, A., Galectins as immunoregulators during infectious processes: From microbial invasion to the resolution of the disease (2005) Parasite Immunol., 27, pp. 103-114
  • Rabinovich, G.A., Ilarregui, J.M., Conveying glycan information into T-cell homeostatic programs: A challenging role for galectin-1 in inflammatory and tumor microenvironments (2009) Immunol. Rev., 230, pp. 144-159
  • Rabinovich, G.A., Toscano, M.A., Turning 'sweet' on immunity: Galectin-glycan interactions in immune tolerance and inflammation (2009) Nat. Rev. Immunol., 9, pp. 338-352
  • Rabinovich, G., Castagna, L., Landa, C., Riera, C.M., Sotomayor, C., Regulated expression of a 16-kd galectin-like protein in activated rat macrophages (1996) J. Leukoc. Biol., 59, pp. 363-370
  • Rabinovich, G.A., Modesti, N.M., Castagna, L.F., Landa, C.A., Riera, C.M., Sotomayor, C.E., Specific inhibition of lymphocyte proliferation and induction of apoptosis by CLL-I, a beta-galactoside-binding lectin (1997) J. Biochem., 122, pp. 365-373
  • Rabinovich, G.A., Iglesias, M.M., Modesti, N.M., Castagna, L.F., Wolfenstein-Todel, C., Riera, C.M., Sotomayor, C.E., Activated rat macrophages produce a galectin-1-like protein that induces apoptosis of T cells: Biochemical and functional characterization (1998) J. Immunol., 160, pp. 4831-4840
  • Rabinovich, G.A., Sotomayor, C.E., Riera, C.M., Bianco, I., Correa, S.G., Evidence of a role for galectin-1 in acute inflammation (2000) Eur. J. Immunol., 30, pp. 1331-1339
  • Rabinovich, G.A., Baum, L.G., Tinari, N., Paganelli, R., Natoli, C., Liu, F.T., Iacobelli, S., Galectins and their ligands: Amplifiers, silencers or tuners of the inflammatory response? (2002) Trends. Immunol., 23, pp. 313-320
  • Rabinovich, G.A., Ramhorst, R.E., Rubinstein, N., Corigliano, A., Daroqui, M.C., Kier-Joffé, E.B., Fainboim, L., Induction of allogenic T-cell hyporesponsiveness by galectin-1-mediated apoptotic and non-apoptotic mechanisms (2002) Cell Death Differ., 9, pp. 661-670
  • Rabinovich, G.A., Liu, F.T., Hirashima, M., Anderson, A., An emerging role for galectins in tuning the immune response: Lessons from experimental models of inflammatory disease, autoimmunity and cancer (2007) Scand. J. Immunol., 66, pp. 143-158
  • Rabinovich, G.A., Toscano, M.A., Jackson, S.S., Vasta, G.R., Functions of cell surface galectin-glycoprotein lattices (2007) Curr. Opin. Struct. Biol., 17, pp. 513-520
  • Rabinovich, G.A., Gabrilovich, D., Sotomayor, E.M., Imunosuppressive strategies that are mediated by tumor cells (2007) Annu. Rev. Immunol., 25, pp. 267-296
  • Reis e Sousa, C., Dendritic cells in a mature age (2006) Nat. Rev. Immunol., 6, pp. 476-483
  • Romero, M.D., Muiño, J.C., Bianco, G.A., Ferrero, M., Juarez, C.P., Luna, J.D., Rabinovich, G.A., Circulating anti-galectin-1 antibodies are associated with the severity of ocular disease in autoimmune and infectious uveitis (2006) Invest. Ophthalmol. Vis. Sci., 47, pp. 1550-1556
  • Rubinstein, N., Alvarez, M., Zwirner, N.W., Toscano, M.A., Ilarregui, J.M., Bravo, A., Mordoh, J., Rabinovich, G.A., Targeted inhibition of galectin-1 gene expression in tumor cells results in heightened T cell-mediated rejection: A potential mechanism of tumor-immune privilege (2004) Cancer Cell, 5, pp. 241-251
  • Sengeløv, H., Follin, P., Kjeldsen, L., Lollike, K., Dahlgren, C., Borregaard, N., Mobilization of granules and secretory vesicles during in vivo exudation of human neutrophils (1995) J. Immunol., 154, pp. 4157-4165
  • Sospedra, M., Martin, R., Immunology of multiple sclerosis (2005) Annu. Rev. Immunol., 23, pp. 683-747
  • Steinman, R.M., Hawiger, D., Liu, K., Bonifaz, L., Bonnyay, D., Mahnke, K., Iyoda, T., Nussenzweig, M., Dendritic cell function in vivo during the steady state: A role in peripheral tolerance (2003) Ann. NY Acad. Sci., 987, pp. 15-25
  • Stillman, B.N., Hsu, D.K., Pang, M., Brewer, C.F., Johnson, P., Liu, F.T., Baum, L.G., Galectin-3 and galectin-1 bind distinct cell surface glycoprotein receptors to induce T cell death (2006) J. Immunol., 176, pp. 778-789
  • Stowell, S.R., Karmakar, S., Arthur, C.M., Ju, T., Rodrigues, L.C., Riul, T.B., Dias-Baruffi, M., Cummings, R.D., Galectin-1 induces reversible phosphatidylserine exposure at the plasma membrane (2009) Mol. Biol. Cell, 20, pp. 1408-1418
  • Strasser, A., Bouillet, P., The control of apoptosis in lymphocyte selection (2003) Immunol. Rev., 193, pp. 82-92
  • Sugimoto, N., Oida, T., Hirota, K., Nakamura, K., Nomura, T., Uchiyama, T., Sakaguchi, S., Foxp3-dependent and -independent molecules specific for CD25+CD4+ natural regulatory T cells revealed by DNA microarray analysis (2006) Int. Immunol., 18, pp. 1197-1209
  • Tarner, I.H., Fathman, C.G., Does our current understanding of the molecular basis of immune tolerance predict new therapies for autoimmune disease? (2006) Nat. Clin. Pract. Rheumatol., 2, pp. 491-499
  • Tateno, H., Uchiyama, N., Kuno, A., Togayachi, A., Sato, T., Narimatsu, H., Hirabayashi, J., A novel strategy for mammalian cell surface glycome profiling using lectin microarray (2007) Glycobiology, 10, pp. 1138-1146
  • Thijssen, V.L., Postel, R., Brandwijk, R.J., Dings, R.P., Nesmelova, I., Satijn, S., Verhofstad, N., Poirier, F., Galectin-1 is essential in tumor angiogenesis and is a target for antiangiogenesis therapy (2006) Proc. Natl. Acad. Sci. USA, 103, pp. 15975-15980
  • Thijssen, V.L., van Beijnum, J.R., Mayo, K.H., Griffioen, A.W., Identification of novel drug targets for angiostatic cancer therapy; it takes two to tango (2007) Curr. Pharm. Des., 13, pp. 3576-3583
  • Toscano, M.A., Commodaro, A.G., Ilarregui, J.M., Bianco, G.A., Liberman, A., Serra, H.M., Hirabayashi, J., Rabinovich, G.A., Galectin-1 suppresses autoimmune retinal disease by promoting concomitant Th2- and T regulatory-mediated anti-inflammatory responses (2006) J. Immunol., 176, pp. 6323-6332
  • Toscano, M.A., Bianco, G.A., Ilarregui, J.M., Croci, D.O., Correale, J., Hernandez, J.D., Zwirner, N.W., Rabinovich, G.A., Differential glycosylation of TH1, TH2 and TH-17 effector cells selectively regulates susceptibility to cell death (2007) Nat. Immunol., 8, pp. 825-834
  • Toscano, M.A., Ilarregui, J.M., Bianco, G.A., Campagna, L., Croci, D.O., Salatino, M., Rabinovich, G.A., Dissecting the pathophysiologic role of endogenous lectins: Glycan-binding proteins with cytokine-like activity? (2007) Cytokine Growth Factor Rev., 18, pp. 57-71
  • van Kooyk, Y., Rabinovich, G.A., Protein-glycan interactions in the control of innate and adaptive immune responses (2008) Nat. Immunol., 9, pp. 593-601
  • Walzel, H., Blach, M., Hirabayashi, J., Kasai, K.I., Brock, J., Involvement of CD2 and CD3 in galectin-1 induced signaling in human Jurkat T-cells (2000) Glycobiology, 10, pp. 131-140
  • Wang, J., Lu, Z.H., Gabius, H.J., Rohowsky-Kochan, C., Ledeen, R.W., Wu, G., Cross-linking of GM1 ganglioside by galectin-1 mediates regulatory T cell activity involving TRPC5 channel activation: Possible role in suppressing experimental autoimmune encephalomyelitis (2009) J. Immunol., 182, pp. 4036-4045
  • Wollina, U., Schreiber, G., Görnig, M., Feldrappe, S., Burchert, M., Gabius, H.J., Sertoli cell expression of galectin-1 and -3 and accessible binding sites in normal human testis and Sertoli cell only-syndrome (1999) Histol. Histopathol., 14, pp. 779-784
  • Yang, R.Y., Rabinovich, G.A., Liu, F.T., Galectins: Structure, function and therapeutic potential (2008) Expert Rev. Mol. Med., 10, pp. e17
  • Young, R.E., Thompson, R.D., Nourshargh, S., Divergent mechanisms of action of the inflammatory cytokines interleukin 1-beta and tumour necrosis factor-alpha in mouse cremasteric venules (2002) Br. J. Pharmacol., 137, pp. 1237-1246
  • Zuñiga, E., Rabinovich, G.A., Iglesias, M.M., Gruppi, A., Regulated expression of galectin-1 during B-cell activation and implications for T-cell apoptosis (2001) J. Leukoc. Biol., 70, pp. 73-79
  • Zuñiga, E., Gruppi, A., Hirabayashi, J., Kasai, K.I., ovich, G.A., Regulated expression and effect of galectin-1 on Trypanosoma cruzi-infected macrophages: Modulation of microbicidal activity and survival (2001) Infect. Immun., 69, pp. 6804-6812

Citas:

---------- APA ----------
Cooper, D., Ilarregui, J.M., Pesoa, S.A., Croci, D.O., Perretti, M. & Rabinovich, G.A. (2010) . Multiple functional targets of the immunoregulatory activity of Galectin-1. Control of immune cell trafficking, dendritic cell physiology, and T-cell fate. Methods in Enzymology, 480(C), 199-244.
http://dx.doi.org/10.1016/S0076-6879(10)80011-4
---------- CHICAGO ----------
Cooper, D., Ilarregui, J.M., Pesoa, S.A., Croci, D.O., Perretti, M., Rabinovich, G.A. "Multiple functional targets of the immunoregulatory activity of Galectin-1. Control of immune cell trafficking, dendritic cell physiology, and T-cell fate" . Methods in Enzymology 480, no. C (2010) : 199-244.
http://dx.doi.org/10.1016/S0076-6879(10)80011-4
---------- MLA ----------
Cooper, D., Ilarregui, J.M., Pesoa, S.A., Croci, D.O., Perretti, M., Rabinovich, G.A. "Multiple functional targets of the immunoregulatory activity of Galectin-1. Control of immune cell trafficking, dendritic cell physiology, and T-cell fate" . Methods in Enzymology, vol. 480, no. C, 2010, pp. 199-244.
http://dx.doi.org/10.1016/S0076-6879(10)80011-4
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Cooper, D., Ilarregui, J.M., Pesoa, S.A., Croci, D.O., Perretti, M., Rabinovich, G.A. Multiple functional targets of the immunoregulatory activity of Galectin-1. Control of immune cell trafficking, dendritic cell physiology, and T-cell fate. Methods Enzymol. 2010;480(C):199-244.
http://dx.doi.org/10.1016/S0076-6879(10)80011-4