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The effect of chronic exposure to Escherichia coli on morphometrical parameters, different antioxidant defenses, lipid peroxidation and protein oxidation has been studied in digestive gland of the freshwater mussel Diplodon chilensis in the laboratory during 6 weeks. In, a parallel field study, we evaluated morphometrical and oxidative stress parameters in digestive glands of mussels collected from a sewage polluted area (SMA) and from a pristine area (control). Both sites were characterized by analyzing bacteriological and physic-chemical parameters. In the laboratory D. chilensis was able to filter bacteria at high concentrations with a clearing rate of 0.510 ± 0.036. L/h per gram of dry soft tissue mass. The digestive gland mass to shell length ratio (DGM/SL), reduced glutathione (GSH), lipid peroxidation, as concentration of thiobartituric acid reactive substances (TBARS), and glutathione-S-transferase (GST) activity of mussels fed with bacteria were significantly higher than those of control mussels after the fourth week. Fecal bacteria in lake water samples were undetectable in the control, and higher than 24,000. MPN (most probable number)/100. mL in SMA. DGM/SL was higher in SMA. No differences between sites were observed in total lipid and protein content, neither in superoxide dismutase activity. GSH content was higher in SMA, with no difference in the oxidized form. GST activity and MDA were significantly higher in SMA but protein oxidation was not affected. © 2011 Elsevier GmbH.


Documento: Artículo
Título:Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis
Autor:Sabatini, S.E.; Rocchetta, I.; Luquet, C.M.; Guido, M.I.; de Molina, M.D.C.R.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pab. II, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia, 1917 Buenos Aires, Argentina
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pab. II, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Laboratorio de Ecotoxicología Acuática, INIBIOMA, CONICET-UNCo, Pasaje de la Paz, 235 San Martín de los Andes, 8370 Neuquén, Argentina
Asentamiento Universitario San Martín de los Andes, Universidad Nacional del Comahue, Pasaje de la Paz, 235 San Martín de los Andes, 8370 Neuquén, Argentina
Palabras clave:Antioxidant defenses; Coliform bacteria; Diplodon chilensis; Oxidative stress; Sewage pollution; antioxidant; coliform bacterium; enzyme activity; freshwater environment; growth; laboratory method; mollusc; morphometry; oxidation; pollution effect; pollution exposure; sewage; Bacteria (microorganisms); Diplodon chilensis; Escherichia coli
Página de inicio:356
Página de fin:362
Título revista:Limnologica
Título revista abreviado:Limnologica


  • Almeida, E.A., Bainy, A.C.D., Loureiro, A.P.M., Medeiros, M.H.G., Di Mascio, P., DNA and lipid damage in the brown mussel Perna perna from a contaminated site (2003) Bull. Environ. Contam. Toxicol., 71, pp. 270-275
  • Anderson, E.M., Determination of glutathione and glutathione disulfide in biological samples (1985) Meth. Enzymol., 70, pp. 548-555
  • (1992) APHA American Water Works Association and Water Pollution Federation. Standard Methods for Examination of Water and Wastewater, , American Public Health Association, Madrid, Spain
  • Bainy, A.C.D., Almeida, E.A., Müller, I.C., Ventura, E.C., Medeiros, I.D., Biochemical responses in farmed mussel Perna perna transplanted to contaminated sites on Santa Catarina Island, SC, Brazil (2000) Mar. Environ. Res., 50, pp. 411-416
  • Ben-Shaul, V., Sofer, Y., Bergman, M., Zurovsky, Y., Grossman, S., Lipopolysaccharide induced oxidative stress in the liver: comparison between rat and rabbit (1999) Shock, 12, pp. 288-293
  • Bligh, E.G., Dyer, W.J., A rapid method of total lipid extraction and purification (1959) Can. J. Biochem. Physiol., 37, pp. 911-917
  • Bonetto, A.A., Nayades de la Patagonia (1973) Rev. Asoc. Cienc. Nat. Litoral., 4, pp. 117-185
  • Bradford, M., A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal. Biochem., 72, pp. 248-254
  • Charissou, A.M., Cossu-Leguille, C., Vasseur, P., Relationship between two oxidative stress biomarkers, malondialdehyde and 8-oxo-7,8-ihydro-29-deoxyguanosine, in the freshwater bivalve Unio tumidus (2004) Sci. Total Environ., 322, pp. 109-122
  • Diaz, M., Pedrozo, F., Reynols, C., Temporetti, P., Chemical composition and the nitrogen-regulated trophic state of Patagonian lakes (2007) Limnologica, 37, pp. 17-27
  • Fitzpatrick, P.J., O'Halloran, J., Sheehan, D., Walsh, A.R., Assessment of a glutathione-S-transferase and related proteins in the gill and digestive gland of Mytilus edulis (L.) as potential organic pollution biomarkers (1997) Biomarkers, 2, pp. 51-56
  • Fraga, C.G., Leibovitz, B.E., Tappel, A.L., Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices. Characterization and comparison with homogenates and microsomes (1988) Free Radic. Biol. Med., 4, pp. 155-161
  • Fridovich, I., Superoxide anion radical, superoxide dismutases, and related matters (1997) J. Biol. Chem., 250, pp. 18515-18517
  • Gagne, F., Blaisea, C., Hellou, J., Endocrine disruption and health effects of caged mussels, Elliptio complanata, placed downstream from a primary-treated municipal effluent plume for 1 year (2004) Comp. Biochem. Physiol. C, 138, pp. 33-44
  • Gifford, S., Dunstan, R.H., O'Connor, W., Macfarlane, G.R., Quantification of in situ nutrient and heavy metal remediation by a small pearl oyster (Pinctada imbricata) farm at Port Stephens, Australia (2005) Mar. Pollut. Bull., 50, pp. 417-422
  • Guinea, J., Sancho, J., Pares, R., (1979) Análisis microbiológico de aguas. Aspectos aplicados, pp. 11-100. , Universidad de Barcelona, Ediciones Omega, Barcelona
  • Habig, W.H., Pabst, M.J., Jakoby, W.B., Glutathione S-transferases: the first enzymatic step in mercapturic acid formation (1974) J. Biol. Chem., 249, pp. 7130-7139
  • Helm, M.M., Holland, D.L., Utting, S.D., East, J., Fatty acids composition of early non-feeding larvae of european flat oyster Ostrea edulis L.J. (1991) Mar. Biol. Assoc. U.K., 71, pp. 675-691
  • Jain, M., Bhalla-Sarin, N., Glyphosate-induced increase in glutathione S-transferase activity and glutathione content in Groundnut (Arachis hypogaea L.) (2001) Pest. Biochem. Physiol., 69, pp. 143-152
  • Jorgensen, C.B., (1990) Bivalve Filter Feeding: Hydrodynamics, Bioenergetics, Physiology and Ecology, p. 140. , Olsen and Olsen
  • Kheir-Eldin, A.A., Motawi, T.K., Gad, M.Z., Abd-ElGawad, H.M., Protective effect of vitamin E, β-carotene and N-acetylcysteine from the brain oxidative stress induced in rats by lipopolysaccharide (2001) Int. J. Biochem. Cell Biol., 33, pp. 475-482
  • Koroleff, F., Simultaneous oxidation of nitrogen and phosphorus compounds by persulfate (1983) Methods of Seawater Analysis, pp. 168-169. , Verlag Chemie, Weinheimer, K. Grosshoff, M. Eberhadt, K. Kremling (Eds.)
  • Lara, G.P., Parada, E., (1991) Seasonal changes in the condition index of Diplodon chilensis chilensis (Gray, 1828) in sandy and muddy substrata. Villarica lake, Chile (39° 18′S; 72° 05′ W), 62, pp. 99-106. , Boletín Sociedad de Biología de Concepción, Chile
  • Lara, G., Contreras, A., Encina, F., La almeja de agua dulce Diplodon chilensis (Bivalvia, Hyriidae) potencial biofiltro para disminuir los niveles de coliformes en pozos. Experimentos de laboratorio (2002) Gayana, 66, pp. 113-118
  • Lindahl, O., Hart, R., Hernroth, B., Kollberg, S., Loo, L.O., Olrog, L., Rehnstam-Holm, A.S., Syversen, U., Improving marine water quality by mussel farming: a profitable solution for Swedish society (2005) Ambio, 34, pp. 131-138
  • Marker, A.F.H., Crowther, C.A., Gunn, R.J.M., Methanol and acetone as solvents for estimating chlorophyll a and phaeopigments by spectrophotometry (1980) Ergerb Limnol., 14, pp. 52-69
  • May, M.J., Leaver, C.J., Oxidative stimulation of glutathione synthesis in Arabidopsis thaliana suspension cultures (1993) Plant Physiol., 103, pp. 621-627
  • Nakamura, Y., Kerciku, F., Effects of filter-feeding bivalves on the distribution of water quality and nutrient cycling in a eutrophic coastal lagoon (2000) J. Mar. Syst., 26, pp. 209-221
  • Nelson, K.A., Leonard, L.A., Posey, M.H., Alphin, T.D., Mallin, M.A., Using transplanted oyster (Crassostrea virginica) beds to improve water quality in small tidal creeks: a pilot study (2004) J. Exp. Mar. Biol. Ecol., 298, pp. 347-368
  • Nicholson, S., Lam, P.K.S., Pollution monitoring in Southeast Asia using biomarkers in the mytilid mussel Perna viridis (Mytilidae: Bivalvia) (2005) Environ. Int., 31, pp. 121-132
  • (1982) OECD Eutrophication of Water: Monitoring, Assessment and Control, , Organisation for Economic Co-operation and Development, Paris
  • Parada, E., Peredo, S., Lara, G., Valdebenito, I., Growth, age and life span of the freshwater mussel Diplodon chilensis chilensis (Gray, 1828) (1989) Arch. Hydrobiol., 115, pp. 563-573
  • Parada, E., Peredo, S., Cárdenas, S., Valdebenito, I., Peredo, M., Diplodon chilensis a potential residual waters depurator on inland water salmonid fishfarms: a laboratory scale study (2008) Gayana, 72 (1), pp. 68-78
  • Pollero, R.J., Brenner, R.R., Effect of the environment and fasting on the lipid and fatty acid composition of Diplodon patagonicus (1981) Lipids, 16, pp. 685-690
  • Reznick, A.Z., Packer, L., Oxidative damage to proteins: spectrophotometric methods for carbonyl assay (1994) Methods Enzymol., 38, pp. 357-363
  • Ribeiro Guevara, S., Bubach, D., Vigliano, P., Lippolt, G., Arribere, M., Heavy metal and other trace elements in native mussel Diplodon chilensis from Northern Patagonia lakes, Argentina (2004) Biol. Trace Elem. Res., 101, pp. 1-19
  • Romero Rojas, J.A., (1999), Calidad del agua 2 da edición. Alfaomega Mexico. Ley provincial 899. Reglamentación del código de aguas de la Provincia del Neuquén. Anexo del boletín oficial edicion No. 2641-19, decreto 790/99; Semenas, L., Brugni, N., Características poblacionales y ciclo de vida de Diplodon chilensis (d'Orbigny, 1835) (Hyriidae, Bivalvia) en el Lago Gutierrez (Patagonia, Argentina) (2002) Ecol. Austral, 12, pp. 29-40
  • Sokal, R.R., Rohlf, F.J., Introducción a la bioestadística (1984) Serie de Biología Fundamental, , España, S.A. Reverté (Ed.)
  • Soto, D., Mena, G., Filter feeding by the freshwater mussel, Diplodon chilensis, as a biocontrol of salmon farming eutrophication (1999) Aquaculture, 171, pp. 65-81
  • Sparnins, V.L., Venegas, P.L., Wattenberg, L.W., Glutathione S-transferase activity: enhancement by compounds initiating chemical carcinogenesis and by dietary constituents (1982) J. Natl. Cancer Inst., 68, pp. 493-496
  • Sugino, K., Dhi, K., Yamada, K., Kawasaki, T., The role of lipid peroxidation in endotoxin-induced hepatic damage and the protective effect of antioxidants (1987) Surgery, 101, pp. 746-752
  • Torres, M.A., Testa, C.P., Gaspari, C., Masutti, M.B., Neves Panitz, C.M., Curi-Pedrosa, R., Alves de Almeida, E., Wilhem Filho, D., Oxidative stress in the mussel Mytella guyanensis from polluted mangroves on Santa Catarina Island, Brazil (2002) Mar. Pollut. Bull., 44, pp. 923-932
  • Valdovinos, C., Pedreros, P., Geographic variations in shell growth rates of mussel Diplodon chilensis from temperate lakes of Chile: implications for biodiversity conservation (2007) Limnologica, 37, pp. 63-75
  • Viarengo, A., Lowe, D., Bolognesi, C., Fabbri, E., Koehler, A., The use of biomarkers in biomonitoring: a 2-tier approach assessing the level of pollutant-induced stress syndrome in sentinel organisms (2007) Comp. Biochem. Physiol. C, 146, pp. 281-300
  • Vidal, M.L., Basseres, A., Narbonne, J.F., Seasonal variations of pollution biomarkers in two populations of Corbicula fluminea (Müller) (2002) Comp. Biochem. Physiol. C, 131, pp. 133-151
  • Viozzi, G.P., Brugni, N.L., Relación parasitaria y nuevos registros de gloquidios de Diplodon chilensis (Unionacea:Hyriidae) en peces de la Patagonia argentina (2001) Neotropica, 47, pp. 3-12
  • Yamamuro, M., Hiratsuka, J., Ishitobi, Y., Seasonal change in a filter-feeding bivalve Musculista senhousia population of a eutrophic estuarine lagoon (2000) J. Mar. Syst., 26, pp. 117-126


---------- APA ----------
Sabatini, S.E., Rocchetta, I., Luquet, C.M., Guido, M.I. & de Molina, M.D.C.R. (2011) . Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis. Limnologica, 41(4), 356-362.
---------- CHICAGO ----------
Sabatini, S.E., Rocchetta, I., Luquet, C.M., Guido, M.I., de Molina, M.D.C.R. "Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis" . Limnologica 41, no. 4 (2011) : 356-362.
---------- MLA ----------
Sabatini, S.E., Rocchetta, I., Luquet, C.M., Guido, M.I., de Molina, M.D.C.R. "Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis" . Limnologica, vol. 41, no. 4, 2011, pp. 356-362.
---------- VANCOUVER ----------
Sabatini, S.E., Rocchetta, I., Luquet, C.M., Guido, M.I., de Molina, M.D.C.R. Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis. Limnologica. 2011;41(4):356-362.