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Abstract:

The effect of chronic exposure to Escherichia coli on morphometrical parameters, different antioxidant defenses, lipid peroxidation and protein oxidation has been studied in digestive gland of the freshwater mussel Diplodon chilensis in the laboratory during 6 weeks. In, a parallel field study, we evaluated morphometrical and oxidative stress parameters in digestive glands of mussels collected from a sewage polluted area (SMA) and from a pristine area (control). Both sites were characterized by analyzing bacteriological and physic-chemical parameters. In the laboratory D. chilensis was able to filter bacteria at high concentrations with a clearing rate of 0.510 ± 0.036. L/h per gram of dry soft tissue mass. The digestive gland mass to shell length ratio (DGM/SL), reduced glutathione (GSH), lipid peroxidation, as concentration of thiobartituric acid reactive substances (TBARS), and glutathione-S-transferase (GST) activity of mussels fed with bacteria were significantly higher than those of control mussels after the fourth week. Fecal bacteria in lake water samples were undetectable in the control, and higher than 24,000. MPN (most probable number)/100. mL in SMA. DGM/SL was higher in SMA. No differences between sites were observed in total lipid and protein content, neither in superoxide dismutase activity. GSH content was higher in SMA, with no difference in the oxidized form. GST activity and MDA were significantly higher in SMA but protein oxidation was not affected. © 2011 Elsevier GmbH.

Registro:

Documento: Artículo
Título:Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis
Autor:Sabatini, S.E.; Rocchetta, I.; Luquet, C.M.; Guido, M.I.; de Molina, M.D.C.R.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pab. II, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia, 1917 Buenos Aires, Argentina
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pab. II, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Laboratorio de Ecotoxicología Acuática, INIBIOMA, CONICET-UNCo, Pasaje de la Paz, 235 San Martín de los Andes, 8370 Neuquén, Argentina
Asentamiento Universitario San Martín de los Andes, Universidad Nacional del Comahue, Pasaje de la Paz, 235 San Martín de los Andes, 8370 Neuquén, Argentina
Palabras clave:Antioxidant defenses; Coliform bacteria; Diplodon chilensis; Oxidative stress; Sewage pollution; antioxidant; coliform bacterium; enzyme activity; freshwater environment; growth; laboratory method; mollusc; morphometry; oxidation; pollution effect; pollution exposure; sewage; Bacteria (microorganisms); Diplodon chilensis; Escherichia coli
Año:2011
Volumen:41
Número:4
Página de inicio:356
Página de fin:362
DOI: http://dx.doi.org/10.1016/j.limno.2011.04.004
Handle:http://hdl.handle.net/20.500.12110/paper_00759511_v41_n4_p356_Sabatini
Título revista:Limnologica
Título revista abreviado:Limnologica
ISSN:00759511
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00759511_v41_n4_p356_Sabatini.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00759511_v41_n4_p356_Sabatini

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Citas:

---------- APA ----------
Sabatini, S.E., Rocchetta, I., Luquet, C.M., Guido, M.I. & de Molina, M.D.C.R. (2011) . Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis. Limnologica, 41(4), 356-362.
http://dx.doi.org/10.1016/j.limno.2011.04.004
---------- CHICAGO ----------
Sabatini, S.E., Rocchetta, I., Luquet, C.M., Guido, M.I., de Molina, M.D.C.R. "Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis" . Limnologica 41, no. 4 (2011) : 356-362.
http://dx.doi.org/10.1016/j.limno.2011.04.004
---------- MLA ----------
Sabatini, S.E., Rocchetta, I., Luquet, C.M., Guido, M.I., de Molina, M.D.C.R. "Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis" . Limnologica, vol. 41, no. 4, 2011, pp. 356-362.
http://dx.doi.org/10.1016/j.limno.2011.04.004
---------- VANCOUVER ----------
Sabatini, S.E., Rocchetta, I., Luquet, C.M., Guido, M.I., de Molina, M.D.C.R. Effects of sewage pollution and bacterial load on growth and oxidative balance in the freshwater mussel Diplodon chilensis. Limnologica. 2011;41(4):356-362.
http://dx.doi.org/10.1016/j.limno.2011.04.004