Artículo

Suárez, O.V.; Cueto, G.R.; Cavia, R.; Villafañe, I.E.G.; Bilenca, D.N.; Edelstein, A.; Martínez, P.; Miguel, S.; Bellomo, C.; Hodara, K.; Padula, P.J.; Busch, M. "Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina" (2003) Memorias do Instituto Oswaldo Cruz. 98(6):727-732
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Abstract:

We studied hantavirus seroprevalence and virus variability in rodent populations in Diego Gaynor, northwest of Buenos Aires province, Argentina. Rodent samplings were conducted in railroads and cropfield borders in March and July 1999, September and December 2000, and March 2001. Antibody detection was performed by an enzyme link immunosorbent assay (ELISA), using the recombinant nucleoprotein of Andes (AND) virus as antigen. Tissue samples were taken from positive antibody individuals in order to confirm the presence of hantavirus genomic material and to identify virus genotypes. Akodon azarae was the most abundant species, followed by Oligoryzomys flavescens, while Calomys laucha and C. musculinus were rarely caught. We found a rate of seroprevalence of 9.3% for a total sample of 291 A. azarae and 13.5% for 37 O. flavescens. After molecular analyses of hantavirus, we confirmed the presence of hantavirus genomic material in 16 individuals with ELISA (+) results and two individuals with ELISA (-). Four amplimers for each species were sequenced and compared to the corresponding sequences of representative hantaviruses. We identified the AND Cent Lee from three O. flavescens, and the Pergamino virus from four A. azarae and from one O. flavescens. A. azarae males had higher seroprevalence than females, and heavier individuals showed higher seroprevalence than lighter ones. We did not find seroprevalence differences according to sex in O. flavescens, although this result may have been produced by the low sample size. The lowest seroprevalence was found in a period of high rodent density, when juveniles prevailed in the population. We found higher seroprevalences than those detected in previous studies for other localities of central Argentina where cases of hantavirus pulmonary syndrome (HPS) have been reported. The presence of AND Cent Lee virus in rodent populations of the study area, which is responsible of HPS cases in central Argentina, suggests that human populations are at risk of HPS disease, although there were not reported cases of this disease until today.

Registro:

Documento: Artículo
Título:Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina
Autor:Suárez, O.V.; Cueto, G.R.; Cavia, R.; Villafañe, I.E.G.; Bilenca, D.N.; Edelstein, A.; Martínez, P.; Miguel, S.; Bellomo, C.; Hodara, K.; Padula, P.J.; Busch, M.
Filiación:Lab. de Ecologia de Poblaciones, Depto. de Ecologia, Genet. y Evol., Pabellón II, Buenos Aires, Argentina
Consejo Nac. Invest. Cie. T., Argentina
Inst. Nac. de Enferm. Infecciosas, Anlis, Malbrán, Argentina
Palabras clave:Argentina; Hantavirus pulmonary syndrome; Seroprevalence; Sigmodontine rodents; nucleoprotein; recombinant protein; virus antigen; virus protein; antibody detection; Argentina; article; enzyme linked immunosorbent assay; gene sequence; genotype; Hantavirus; Hantavirus pulmonary syndrome; histopathology; molecular biology; nonhuman; railway; rodent; seroprevalence; species comparison; virus genome; virus identification; Akodon; Akodon azarae; Andes virus; Calomys; Calomys laucha; Calomys musculinus; Convict Creek 107 virus; Hantaan virus Lee; Hantavirus; Oligoryzomys; Oligoryzomys flavescens; Pergamino virus; Rodentia
Año:2003
Volumen:98
Número:6
Página de inicio:727
Página de fin:732
DOI: http://dx.doi.org/10.1590/S0074-02762003000600003
Título revista:Memorias do Instituto Oswaldo Cruz
Título revista abreviado:Mem. Inst. Oswaldo Cruz
ISSN:00740276
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00740276_v98_n6_p727_Suarez.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00740276_v98_n6_p727_Suarez

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Citas:

---------- APA ----------
Suárez, O.V., Cueto, G.R., Cavia, R., Villafañe, I.E.G., Bilenca, D.N., Edelstein, A., Martínez, P.,..., Busch, M. (2003) . Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina. Memorias do Instituto Oswaldo Cruz, 98(6), 727-732.
http://dx.doi.org/10.1590/S0074-02762003000600003
---------- CHICAGO ----------
Suárez, O.V., Cueto, G.R., Cavia, R., Villafañe, I.E.G., Bilenca, D.N., Edelstein, A., et al. "Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina" . Memorias do Instituto Oswaldo Cruz 98, no. 6 (2003) : 727-732.
http://dx.doi.org/10.1590/S0074-02762003000600003
---------- MLA ----------
Suárez, O.V., Cueto, G.R., Cavia, R., Villafañe, I.E.G., Bilenca, D.N., Edelstein, A., et al. "Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina" . Memorias do Instituto Oswaldo Cruz, vol. 98, no. 6, 2003, pp. 727-732.
http://dx.doi.org/10.1590/S0074-02762003000600003
---------- VANCOUVER ----------
Suárez, O.V., Cueto, G.R., Cavia, R., Villafañe, I.E.G., Bilenca, D.N., Edelstein, A., et al. Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina. Mem. Inst. Oswaldo Cruz. 2003;98(6):727-732.
http://dx.doi.org/10.1590/S0074-02762003000600003