Artículo

Agusti, R.; Couto, A.S.; Alves, M.J.M.; Colli, W.; De Lederkremer, R.M. "Lipids Shed into the Culture Medium by Trypomastigotes of Trypanosoma cruzi" (2000) Memorias do Instituto Oswaldo Cruz. 95(1-2):97-102
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Abstract:

Trypomastigote forms of Trypanosoma cruzi were metabolically labeled with [14C]-ethanolamine and [3H]-palmitic acid. Lipids shed to the culture medium were analyzed and compared with the parasite components. Phosphatidylcholine and lysophosphatidylcholine accounted for 53% of the total incorporated precursor. Interestingly, phosphatidylethanolamine and its lyso derivative lysophosphatidylethanolamine, although present in significant amounts in the parasites, could not be detected in the shed material. Shed lipids were highly enriched in the desaturated fatty acids C16:1 and C18:1 when compared to the total fatty acid pool isolated from the parasites.

Registro:

Documento: Artículo
Título:Lipids Shed into the Culture Medium by Trypomastigotes of Trypanosoma cruzi
Autor:Agusti, R.; Couto, A.S.; Alves, M.J.M.; Colli, W.; De Lederkremer, R.M.
Filiación:Cihidecar, Depto. de Quimica Orgánica, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina
Departamento de Bioquímica, Institute de Química, Universidade de São Paulo, Caixa Postal 26077, 05599-970 Sáo Paulo, SP, Brazil
Palabras clave:Desaturated fatty acids; Phospholipids; [14C]-ethanolamine and [3H]-palmitic acid incorporation; Trypanosoma; Trypanosoma cruzi; ethanolamine derivative; fatty acid; lipid; lysophosphatidylcholine; palmitic acid; phosphatidylcholine; unsaturated fatty acid; animal; article; chemistry; culture medium; lipid metabolism; metabolism; thin layer chromatography; Trypanosoma cruzi; Animals; Chromatography, Thin Layer; Culture Media; Ethanolamines; Fatty Acids, Nonesterified; Fatty Acids, Unsaturated; Lipid Metabolism; Lipids; Lysophosphatidylcholines; Palmitic Acid; Phosphatidylcholines; Trypanosoma cruzi
Año:2000
Volumen:95
Número:1-2
Página de inicio:97
Página de fin:102
DOI: http://dx.doi.org/10.1590/S0074-02762000000100016
Título revista:Memorias do Instituto Oswaldo Cruz
Título revista abreviado:Mem. Inst. Oswaldo Cruz
ISSN:00740276
CAS:Culture Media; Ethanolamines; Fatty Acids, Nonesterified; Fatty Acids, Unsaturated; Lipids; Lysophosphatidylcholines; Palmitic Acid, 57-10-3; Phosphatidylcholines
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00740276_v95_n1-2_p97_Agusti

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Citas:

---------- APA ----------
Agusti, R., Couto, A.S., Alves, M.J.M., Colli, W. & De Lederkremer, R.M. (2000) . Lipids Shed into the Culture Medium by Trypomastigotes of Trypanosoma cruzi. Memorias do Instituto Oswaldo Cruz, 95(1-2), 97-102.
http://dx.doi.org/10.1590/S0074-02762000000100016
---------- CHICAGO ----------
Agusti, R., Couto, A.S., Alves, M.J.M., Colli, W., De Lederkremer, R.M. "Lipids Shed into the Culture Medium by Trypomastigotes of Trypanosoma cruzi" . Memorias do Instituto Oswaldo Cruz 95, no. 1-2 (2000) : 97-102.
http://dx.doi.org/10.1590/S0074-02762000000100016
---------- MLA ----------
Agusti, R., Couto, A.S., Alves, M.J.M., Colli, W., De Lederkremer, R.M. "Lipids Shed into the Culture Medium by Trypomastigotes of Trypanosoma cruzi" . Memorias do Instituto Oswaldo Cruz, vol. 95, no. 1-2, 2000, pp. 97-102.
http://dx.doi.org/10.1590/S0074-02762000000100016
---------- VANCOUVER ----------
Agusti, R., Couto, A.S., Alves, M.J.M., Colli, W., De Lederkremer, R.M. Lipids Shed into the Culture Medium by Trypomastigotes of Trypanosoma cruzi. Mem. Inst. Oswaldo Cruz. 2000;95(1-2):97-102.
http://dx.doi.org/10.1590/S0074-02762000000100016