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Abstract:

This study reports on the standardization of an enzyme-linked immunosorbent assay (ELISA) for detecting specific antibodies anti-Trypanosoma cruzi in naturally infected dogs. Sera from 182 mongrel dogs of all ages residing in four rural villages in Santiago del Estera, Argentina, were collected in November 1994 and preserved in buffered neutral glycerin. All sera were tested by indirect hemagglutination test (IHAT), indirect immunofluorescence test (IFAT), and ELISA using the flagellar fraction of T. cruzi as antigen. Dog sera from an area without vectorial transmission were used to calculate ELISA specificity and cut-off value. Eighty-six percent of sera had concordant results for all tests. All sera reactive for IHAT and IFAT were also reactive for ELISA, except in one case. Sera tested by ELISA when diluted 1 : 200 allowed a clearer division between non-reactive and reactive sera than when 1 : 100 with greater agreement among serologic techniques. The specificity of ELISA was 96.2%. Among 34 adult dogs with a positive xenodiagnosis, sensitivity was 94% both for ELISA and IFAT. ELISA is the first choice for screening purposes and one of the pair of techniques recommended for diagnostic studies in dog populations.

Registro:

Documento: Artículo
Título:Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs
Autor:Lauricella, M.A.; Castañera, M.B.; Gürtler, R.E.; Segura, E.L.
Filiación:Inst. Nac. Parasitol. Dr. Mario F., Avenida Paseo Colón 568, 1063 Buenos Aires, Argentina
Depto. de Ciencias Biológicas, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Admin. Nac. Laboratorios I., Av. Vélez Sarsfield 563, 1281 Buenos Aires, Argentina
Palabras clave:Chronic infection; Dogs; Serology; Surveillance; Trypanosoma cruzi; Canis familiaris; Trypanosoma; Trypanosoma cruzi; protozoon antibody; animal; animal disease; Argentina; article; blood; Chagas disease; dog; dog disease; enzyme linked immunosorbent assay; hemagglutination test; immunology; sensitivity and specificity; standard; Trypanosoma cruzi; Animals; Antibodies, Protozoan; Argentina; Chagas Disease; Dog Diseases; Dogs; Enzyme-Linked Immunosorbent Assay; Hemagglutination Tests; Sensitivity and Specificity; Trypanosoma cruzi
Año:1998
Volumen:93
Número:4
Página de inicio:501
Página de fin:507
DOI: http://dx.doi.org/10.1590/S0074-02761998000400016
Título revista:Memorias do Instituto Oswaldo Cruz
Título revista abreviado:Mem. Inst. Oswaldo Cruz
ISSN:00740276
CAS:Antibodies, Protozoan
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00740276_v93_n4_p501_Lauricella.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00740276_v93_n4_p501_Lauricella

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Citas:

---------- APA ----------
Lauricella, M.A., Castañera, M.B., Gürtler, R.E. & Segura, E.L. (1998) . Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs. Memorias do Instituto Oswaldo Cruz, 93(4), 501-507.
http://dx.doi.org/10.1590/S0074-02761998000400016
---------- CHICAGO ----------
Lauricella, M.A., Castañera, M.B., Gürtler, R.E., Segura, E.L. "Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs" . Memorias do Instituto Oswaldo Cruz 93, no. 4 (1998) : 501-507.
http://dx.doi.org/10.1590/S0074-02761998000400016
---------- MLA ----------
Lauricella, M.A., Castañera, M.B., Gürtler, R.E., Segura, E.L. "Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs" . Memorias do Instituto Oswaldo Cruz, vol. 93, no. 4, 1998, pp. 501-507.
http://dx.doi.org/10.1590/S0074-02761998000400016
---------- VANCOUVER ----------
Lauricella, M.A., Castañera, M.B., Gürtler, R.E., Segura, E.L. Immunodiagnosis of Trypanosoma cruzi (Chagas' Disease) Infection in Naturally Infected Dogs. Mem. Inst. Oswaldo Cruz. 1998;93(4):501-507.
http://dx.doi.org/10.1590/S0074-02761998000400016