Artículo

Ferrari, I.; Lorenzi, H.; Santos, M.R.; Brandariz, S.; Requena, J.M.; Schijman, A.; Vázquez, M.; Da Silveira, J.F.; Ben-Dov, C.; Medrano, C.; Ghío, S.; López Bergami, P.; Cano, I.; Zingales, B.; Urmenyi, T.P.; Rondinelli, E.; González, A.; Cortes, A. (...) Levin, M.J. "Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener" (1997) Memorias do Instituto Oswaldo Cruz. 92(6):843-852
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Abstract:

Strategies to construct the physical map of the Trypanosoma cruzi nuclear genome have to capitalize on three main advantages of the parasite genome, namely (a) its small size, (b) the fact that all chromosomes can be defined, and many of them can be isolated by pulse field gel electrophoresis, and (c) the fact that simple Southern blots of electrophoretic karyotypes can be used to map sequence tagged sites and expressed sequence tags to chromosomal bands. A major drawback to cope with is the complexity of T. cruzi genetics, that hinders the construction of a comprehensive genetic map. As a first step towards physical mapping, we report the construction and partial characterization of a T. cruzi CL-Brener genomic library in yeast artificial chromosomes (YACs) that consists of 2,770 individual YACs with a mean insert size of 365 kb encompassing around 10 genomic equivalents. Two libraries in bacterial artificial chromosomes (BACs) have been constructed, BACI and BACII. Both libraries represent about three genome equivalents. A third BAC library (BAC III) is being constructed. YACs and BACs are invaluable tools for physical mapping. More generally, they have to be considered as a common resource for research in Chagas disease.

Registro:

Documento: Artículo
Título:Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener
Autor:Ferrari, I.; Lorenzi, H.; Santos, M.R.; Brandariz, S.; Requena, J.M.; Schijman, A.; Vázquez, M.; Da Silveira, J.F.; Ben-Dov, C.; Medrano, C.; Ghío, S.; López Bergami, P.; Cano, I.; Zingales, B.; Urmenyi, T.P.; Rondinelli, E.; González, A.; Cortes, A.; Lopez, M.C.; Thomas, M.C.; Alonso, C.; Ramírez, J.L.; Chiurrillo, M.A.; Rangel Aldao, R.; Brandão, A.; Degrave, W.; Perrot, V.; Saumier, M.; Billaut, A.; Cohen, D.; Le Paslier, D.; Levin, M.J.
Filiación:Inst. Invest. Ing. Genet. y Biol. M., Obligado 2490, 1428 Buenos Aires, Argentina
Escola Paulista de Medicina, Rua Botucatu 862, São Paulo, SP, Brazil
CBM, Madrid, Spain
Universidade de São Paulo, São Paulo, SP, Brazil
Univ. Federal do Rio de Janeiro, CCS, Ilha do Fundão, Rio de Janeiro, RJ, Brazil
IPB, Granada, Spain
Universidad Central de Venezuela, Apdo 47525, Caracas, Venezuela
USB, Sartenejas, Caracas, Venezuela
Instituto Oswaldo Cruz, Av. Brasil 4365, Rio de Janeiro, RJ, Brazil
CEPH, 27 Rue Juliette Dodou, 75010 Paris, France
Palabras clave:BAC library; Expressed sequence tags (EST); Genome project; Physical map; Sequence tagged sites (STS); Trypanosoma cruzi; YAC library; Bacteria (microorganisms); Trypanosoma; Trypanosoma cruzi; animal; article; bacterial chromosome; chromosome map; cloning; gene library; genetics; genome; Trypanosoma cruzi; yeast artificial chromosome; Animals; Chromosome Mapping; Chromosomes, Artificial, Yeast; Chromosomes, Bacterial; Cloning, Organism; Genome, Protozoan; Genomic Library; Trypanosoma cruzi
Año:1997
Volumen:92
Número:6
Página de inicio:843
Página de fin:852
DOI: http://dx.doi.org/10.1590/S0074-02761997000600021
Título revista:Memorias do Instituto Oswaldo Cruz
Título revista abreviado:Mem. Inst. Oswaldo Cruz
ISSN:00740276
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00740276_v92_n6_p843_Ferrari

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Citas:

---------- APA ----------
Ferrari, I., Lorenzi, H., Santos, M.R., Brandariz, S., Requena, J.M., Schijman, A., Vázquez, M.,..., Levin, M.J. (1997) . Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener. Memorias do Instituto Oswaldo Cruz, 92(6), 843-852.
http://dx.doi.org/10.1590/S0074-02761997000600021
---------- CHICAGO ----------
Ferrari, I., Lorenzi, H., Santos, M.R., Brandariz, S., Requena, J.M., Schijman, A., et al. "Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener" . Memorias do Instituto Oswaldo Cruz 92, no. 6 (1997) : 843-852.
http://dx.doi.org/10.1590/S0074-02761997000600021
---------- MLA ----------
Ferrari, I., Lorenzi, H., Santos, M.R., Brandariz, S., Requena, J.M., Schijman, A., et al. "Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener" . Memorias do Instituto Oswaldo Cruz, vol. 92, no. 6, 1997, pp. 843-852.
http://dx.doi.org/10.1590/S0074-02761997000600021
---------- VANCOUVER ----------
Ferrari, I., Lorenzi, H., Santos, M.R., Brandariz, S., Requena, J.M., Schijman, A., et al. Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener. Mem. Inst. Oswaldo Cruz. 1997;92(6):843-852.
http://dx.doi.org/10.1590/S0074-02761997000600021