Artículo

Santos, M.R.M.; Cano, M.I.; Schijman, A.; Lorenzi, H.; Vázquez, M.; Levin, M.J.; Ramirez, J.L.; Brandão, A.; Degrave, W.M.; Da Silveira, J.F. "The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener" (1997) Memorias do Instituto Oswaldo Cruz. 92(6):821-828
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Abstract:

By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference clone CL Brener selected for Trypanosoma cruzi genome project was established. A total of 20 uniform chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybridized with 39 different homologous probes, 13 of which identified single chromosomes. Several markers showed linkage and four different linkage groups were identified, each comprising two markers. Densitometric analysis suggests that most of the chromosomal bands contain two or more chromosomes representing either homologous chromosomes and/or heterologous chromosomes with similar sizes.

Registro:

Documento: Artículo
Título:The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
Autor:Santos, M.R.M.; Cano, M.I.; Schijman, A.; Lorenzi, H.; Vázquez, M.; Levin, M.J.; Ramirez, J.L.; Brandão, A.; Degrave, W.M.; Da Silveira, J.F.
Filiación:Univ. Federal de São Paulo, Rua Botucatu 862, 04023-062, São Paulo, SP, Brazil
Inst. Invest. Ing. Genet. y Biol. M., INGEBI-CONICET, FCEyN-UBA, Obligado 2490, 1428 Buenos Aires, Argentina
Universidad Central de Venezuela, Caracas, Venezuela
Instituto Oswaldo Cruz, Av. Brasil 4365, 21045-900, Rio de Janeiro, RJ, Brazil
Palabras clave:Chromosome mapping; Clone CL Brener; Genetic map; Genome project; Molecular karyotype; Trypanosoma cruzi; Trypanosoma; Trypanosoma cruzi; animal; chromosome map; cloning; genetics; genome; karyotyping; review; Trypanosoma cruzi; Animals; Chromosome Mapping; Cloning, Organism; Genome, Protozoan; Karyotyping; Trypanosoma cruzi
Año:1997
Volumen:92
Número:6
Página de inicio:821
Página de fin:828
DOI: http://dx.doi.org/10.1590/S0074-02761997000600018
Título revista:Memorias do Instituto Oswaldo Cruz
Título revista abreviado:Mem. Inst. Oswaldo Cruz
ISSN:00740276
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00740276_v92_n6_p821_Santos.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00740276_v92_n6_p821_Santos

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Citas:

---------- APA ----------
Santos, M.R.M., Cano, M.I., Schijman, A., Lorenzi, H., Vázquez, M., Levin, M.J., Ramirez, J.L.,..., Da Silveira, J.F. (1997) . The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener. Memorias do Instituto Oswaldo Cruz, 92(6), 821-828.
http://dx.doi.org/10.1590/S0074-02761997000600018
---------- CHICAGO ----------
Santos, M.R.M., Cano, M.I., Schijman, A., Lorenzi, H., Vázquez, M., Levin, M.J., et al. "The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener" . Memorias do Instituto Oswaldo Cruz 92, no. 6 (1997) : 821-828.
http://dx.doi.org/10.1590/S0074-02761997000600018
---------- MLA ----------
Santos, M.R.M., Cano, M.I., Schijman, A., Lorenzi, H., Vázquez, M., Levin, M.J., et al. "The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener" . Memorias do Instituto Oswaldo Cruz, vol. 92, no. 6, 1997, pp. 821-828.
http://dx.doi.org/10.1590/S0074-02761997000600018
---------- VANCOUVER ----------
Santos, M.R.M., Cano, M.I., Schijman, A., Lorenzi, H., Vázquez, M., Levin, M.J., et al. The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener. Mem. Inst. Oswaldo Cruz. 1997;92(6):821-828.
http://dx.doi.org/10.1590/S0074-02761997000600018