Artículo

Loffler, S.G.; Pavan, M.E.; Vanasco, B.; Samartino, L.; Suarez, O.; Auteri, C.; Romero, G.; Brihuega, B. "Genotypes of pathogenic Leptospira spp isolated from rodents in Argentina" (2014) Memorias do Instituto Oswaldo Cruz. 109(2):163-167
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Leptospirosis is the most widespread zoonosis in the world and significant efforts have been made to determine and classify pathogenic Leptospira strains. This zoonosis is maintained in nature through chronic renal infections of carrier animals, with rodents and other small mammals serving as the most important reservoirs. Additionally, domestic animals, such as livestock and dogs, are significant sources of human infection. In this study, a multiple-locus variable-number tandem repeat analysis (MLVA) was applied to genotype 22 pathogenic Leptospira strains isolated from urban and periurban rodent populations from different regions of Argentina. Three MLVA profiles were identified in strains belonging to the species Leptospira interrogans (serovars Icterohaemorrhagiae and Canicola); one profile was observed in serovar Icterohaemorrhagiae and two MLVA profiles were observed in isolates of serovars Canicola and Portlandvere. All strains belonging to Leptospira borgpetersenii serovar Castellonis exhibited the same MLVA profile. Four different genotypes were isolated from urban populations of rodents, including both mice and rats and two different genotypes were isolated from periurban populations.

Registro:

Documento: Artículo
Título:Genotypes of pathogenic Leptospira spp isolated from rodents in Argentina
Autor:Loffler, S.G.; Pavan, M.E.; Vanasco, B.; Samartino, L.; Suarez, O.; Auteri, C.; Romero, G.; Brihuega, B.
Filiación:Laboratorio de Leptospirosis, Instituto de Patobiología, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria Castelar, Buenos Aires, Argentina
Laboratorio de Biología Molecular, Biochemiq SA, Buenos Aires, Argentina
Instituto Nacional de Enfermedades Respiratorias Dr E Coni, Administración Nacional de Laboratorios e Institutos de Salud Dr Carlos G Malbrán, Santa Fe, Argentina
Bacteriología y Micología, Universidad del Salvador, Pilar, Buenos Aires, Argentina
Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Argentina; Genotyping; Leptospira spp; Multiple-locus variable-number tandem repeat analysis; Pathogenic; Rodent population; animal; Argentina; classification; Didelphis; genetics; genotype; genotyping technique; isolation and purification; Leptospira; Leptospira interrogans serovar Canicola; Leptospira interrogans serovar Icterohaemorrhagiae; Leptospira interrogans serovar Pomona; leptospirosis; microbiology; mouse; procedures; rat; rodent; serotype; serotyping; tandem repeat; transmission; urban population; virulence; Animals; Argentina; Didelphis; Genotype; Genotyping Techniques; Leptospira; Leptospira interrogans serovar canicola; Leptospira interrogans serovar icterohaemorrhagiae; Leptospira interrogans serovar pomona; Leptospirosis; Mice; Rats; Rodentia; Serogroup; Serotyping; Tandem Repeat Sequences; Urban Population; Virulence
Año:2014
Volumen:109
Número:2
Página de inicio:163
Página de fin:167
DOI: http://dx.doi.org/10.1590/0074-0276140295
Título revista:Memorias do Instituto Oswaldo Cruz
Título revista abreviado:Mem. Inst. Oswaldo Cruz
ISSN:00740276
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00740276_v109_n2_p163_Loffler

Referencias:

  • Adler, B., Moctezuma, A.P., Leptospira and leptospirosis (2010) Vet Microbiol, 27, pp. 287-296
  • Arango, J., Cittadino, E., Agostini, A., De Mazzonelli, G.D., Alvarez, C., Colusi, M., Koval, A., Kravetz, F., Prevalencia de leptospiras en Rattus rattus y Rattus norvegicus en el gran Buenos Aires, Argentina (2001) Ecol Austral, 11, pp. 25-30
  • Brihuega, B., Pavan, M., Cairó, F., Venzano, A., Auteri, C., Funes, D., Romero, G., Samartino, L., Leptospira patógena en riñon de Didelphis albiventris (comadreja) (2007) Rev Argent Microbiol, 39, p. 19
  • Cacchione, R.A., Enfoques de los estudios de las leptospirosis humana y animal en América Latina (1973) Rev Argent Microbiol, 1, pp. 36-53
  • Cacchione, R.A., Bulgini, M.J.D., Cascelli, E.S., Martinez, E.S., Leptospirosis en animales silvestres. Estado actual de sus investigaciones, aislamientos y clasificación de cepas argentinas (1967) Rev Fac C Vet, 20, pp. 37-54
  • Cerqueira, G.M., McBride, A.J., Picardeau, M., Ribeiro, S.G., Moreira, A.N., Morel, V., Reis, M.G., Dellagostin, O.A., Distribution of the leptospiral immunoglobin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates (2009) J Med Microbiol, 58, pp. 1173-1181
  • Cerqueira, G.M., Picardeau, M., A century of Leptospira strain typing (2009) Infect Genet Evol, 9, pp. 760-768
  • Draghi, M.G., Brihuega, B., Benítez, D., Sala, J.M., Biotti, G.M., Pereyra, M., Homse, A., Guariniello, L., Brote de leptospirosis en terneros en recría en la provincia de Corrientes, Argentina (2011) Rev Argent Microbiol, 43, pp. 42-44
  • Hartskeerl, R.A., Collares-Pereira, M., Ellis, W.A., Emergence, control and re-emerging leptospirosis: Dynamics of infection in the changing world (2011) Clin Microbiol Infect, 17, pp. 494-501
  • Jorge, S., Hartleben, C., Seixas, F., Coimbra, M., Stark, C., Larrondo, A., Amaral, M., Brod, C.S., Leptospira borgpetersenii from free-living white-eared opossum (Didelphis albiventris): First isolation in Brazil (2012) Acta Trop, 124, pp. 147-151
  • Levett, P.N., Leptospirosis (2001) Clin microbiol Rev, 14, pp. 296-326
  • Levett, P.N., Haake, D.A., Leptospira species (leptospirosis) (2010) Principles and practice of infectious diseases, pp. 3059-3065. , In GL Mandell, JE Bennett, R Dolin (eds.), Churchill Livingtsone Elsevier, Philadelphia
  • Li, S.J., Zhang, C.C., Li, X.W., Tian, K.C., Tang, G.P., Wang, D.M., Liu, Y., Jiang, X.G., Molecular typing of Leptospira interrogans strains isolated from Rattus tanezumi in Guizhou province, South West of China (2012) Biomed Enviorn Sci, 25, pp. 542-548
  • Majed, Z., Bellenger, E., Postic, D., Pourcel, C., Baranton, G., Picardeau, M., Identification of variable-number tandem-repeat loci in Leptospira interrogans sensu stricto (2005) J Clin Microbiol, 43, pp. 539-545
  • Marder, G., Ruiz, R.M., Bottinelli, O.R., Peiretti, H.A., Zorzo, L., Merino, D.E., Czernik, G.E., Prevalencia de leptospirosis en roedores sinantrópicos de la Cuidad de Corrientes, Argentina (2008) Rev Vet, 19, pp. 150-153
  • Martin, U., Sensevy, A., Colombo, J., Tramontin, V., Leptospirosis en la Provincia de Santa Fe-Descripción epidemiológica, clínica y socioeconómica (2002) Medicina, 62, pp. 164-168
  • (2013) Boletín integrado de vigilancia 177 SE 26, p. 123. , MS-Ministerio de Salud Argentina, Secretaria de Promoción e Programas Sanitários, Buenos Aires
  • Nalam, K., Ahmed, A., Devi, S.M., Francalacci, P., Baig, M., Sechi, L.A., Hartskeerl, R.A., Ahmed, N., Genetic affinities within a large global collection of pathogenic leptospira: Implications for strain identification and molecular epidemiology (2010) PLoS ONE, 5, pp. e12637
  • Pavan, M.E., Brihuega, B., Pettinari, M.J., Cairó, F., Multiple-locus variable-number tandem repeat analysis of reference strains used for the diagnosis of leptospirosis in Argentina (2011) Rev Argent Microbiol, 43, pp. 251-255
  • Pavan, M.E., Cairó, F., Brihuega, B., Samartino, L., Multiple-locus variable-number tandem repeat analysis (MLVA) of Leptospira interrogans serovar Pomona from Argentina reveals four new genotypes (2008) Comp Immunol Microbiol Infect Dis, 31, pp. 37-45
  • Pavan, M.E., Cairó, F., Pettinari, M.J., Samartino, L., Brihuega, B., Genotyping of Leptospira interrogans strains from Argentina by multiple-locus variable-number tándem repeat analysis (MLVA) (2011) Comp Immunol Microbiol Infect Dis, 34, pp. 135-141
  • Perez, J., Brescia, F., Becam, J., Mauron, C., Goarant, C., Rodent abundance dynamics and leptospirosis carriage in an area of hyper. Endemicity in New Caledonia (2011) PLoS Negl Trop Dis, 5, pp. e1361
  • Salaün, L., Mérien, F., Gurianova, S., Baranton, G., Picardeau, M., Application of multilocus variable-number tandem-repeat analysis for molecular typing of the agent of leptospirosis (2006) J Clin Microbiol, 44, pp. 3954-3962
  • Scialfa, E., Bolpe, J., Bardon, J.C., Ridao, G., Gentile, J., Gallicchio, O., Isolation of Leptospira interrogans from suburban rats in Tandil, Buenos Aires (2010) Rev Argent Microbiol, 42, pp. 126-128
  • Slack, A.T., Dohnt, M.F., Symonds, M.L., Smythe, L.D., Development of a multiple-locus variable number of tandem repeat analysis (MLVA) for Leptospira interrogans and its application to Leptospira interrogans serovar Australis isolates from far North Queensland, Australia (2005) Ann Clin Microbiol Antimicrob, 4, p. 10
  • Slack, A.T., Symonds, M., Dohnt, M., Smythe, L., An improved multiple-locus variable number of tandem repeats analysis for Leptospira interrogans serovar Australis: A comparison with fluorescent amplified fragment length polymorphism analysis and its use to redefine the molecular epidemiology of this serovar in Queensland, Australia (2006) J Med Microbiol, 55, pp. 1549-1557
  • Vanasco, N.B., Rossetti, C., Sequeira, G., Sequeira, M.D., Calderón, G., Tarabla, H.D., First isolations of leptospires serogroup Ballum serovar arbórea in Argentina (2000) Vet Rec, 147, pp. 246-247
  • Vanasco, N.B., Sequeira, M.D., Sequeira, G., Tarabla, H.D., Associations between leptospiral infection and seropositivity in rodents and environmental characteristics in Argentina (2003) Prev Vet Med, 60, pp. 227-235
  • Vedhagiri, K., Natarajaseenivasan, K., Prabhakaran, S.G., Selvin, J., Narayanan, R., Shouche, Y., Vijayachari, P., Ratnam, S., Characterization of Leptospira borgpetersenii isolates from field rats (Rattus norvegicus) by 16S RRNA and LipL32 gene sequences (2010) Braz J Microbiol, 41, pp. 150-157
  • Zuerner, R.L., Alt, D.T., Variable nucleotide tandem-repeat analysis revealing a unique group of Leptospira interrogans serovars Pomona isolates associated with California sea lions (2009) J Clin Microbiol, 47, pp. 1202-1205

Citas:

---------- APA ----------
Loffler, S.G., Pavan, M.E., Vanasco, B., Samartino, L., Suarez, O., Auteri, C., Romero, G.,..., Brihuega, B. (2014) . Genotypes of pathogenic Leptospira spp isolated from rodents in Argentina. Memorias do Instituto Oswaldo Cruz, 109(2), 163-167.
http://dx.doi.org/10.1590/0074-0276140295
---------- CHICAGO ----------
Loffler, S.G., Pavan, M.E., Vanasco, B., Samartino, L., Suarez, O., Auteri, C., et al. "Genotypes of pathogenic Leptospira spp isolated from rodents in Argentina" . Memorias do Instituto Oswaldo Cruz 109, no. 2 (2014) : 163-167.
http://dx.doi.org/10.1590/0074-0276140295
---------- MLA ----------
Loffler, S.G., Pavan, M.E., Vanasco, B., Samartino, L., Suarez, O., Auteri, C., et al. "Genotypes of pathogenic Leptospira spp isolated from rodents in Argentina" . Memorias do Instituto Oswaldo Cruz, vol. 109, no. 2, 2014, pp. 163-167.
http://dx.doi.org/10.1590/0074-0276140295
---------- VANCOUVER ----------
Loffler, S.G., Pavan, M.E., Vanasco, B., Samartino, L., Suarez, O., Auteri, C., et al. Genotypes of pathogenic Leptospira spp isolated from rodents in Argentina. Mem. Inst. Oswaldo Cruz. 2014;109(2):163-167.
http://dx.doi.org/10.1590/0074-0276140295