Artículo

Nicolao, M.C.; Elissondo, M.C.; Denegri, G.M.; Goya, A.B.; Cumino, A.C. "In Vitro and in Vivo effects of tamoxifen against larval stage Echinococcus granulosus" (2014) Antimicrobial Agents and Chemotherapy. 58(9):5146-5154
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Abstract:

Cystic echinococcosis is a zoonotic infection caused by the larval stage of the cestode Echinococcus granulosus. Chemotherapy currently employs benzimidazoles; however, 40% of cases do not respond favorably. With regard to these difficulties, novel therapeutic tools are needed to optimize treatment in humans. The aim of this work was to explore the in vitro and in vivo effects of tamoxifen (TAM) against E. granulosus. In addition, possible mechanisms for the susceptibility of TAM are discussed in relation to calcium homeostasis, P-glycoprotein inhibition, and antagonist effects on a putative steroid receptor. After 24 h of treatment, TAM, at a low micromolar concentration range (10 to 50 μM), inhibited the survival of E. granulosus protoscoleces and metacestodes. Moreover, we demonstrated the chemotherapeutic and chemopreventive pharmacological effects of the drug. At a dose rate of 20 mg/kg of body weight, TAM induced protection against the infection in mice. In the clinical efficacy studies, a reduction in cyst weight was observed after the administration of 20 mg/kg in mice with cysts developed during 3 or 6 months, compared to that of those collected from control mice. Since the collateral effects of high TAM doses have been largely documented in clinical trials, the use of low doses of this drug as a short-term therapy may be a novel alternative approach for human cystic echinococcosis treatment. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Registro:

Documento: Artículo
Título:In Vitro and in Vivo effects of tamoxifen against larval stage Echinococcus granulosus
Autor:Nicolao, M.C.; Elissondo, M.C.; Denegri, G.M.; Goya, A.B.; Cumino, A.C.
Filiación:Departamento de Biología, Facultad de Ciencias Exactas Y Naturales, Universidad Nacional de Mar del Plata (UNMdP), Mar del Plata, Argentina
Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET), Buenos Aires, Argentina
Departamento de Toxinas Marinas, Centro Regional Buenos Aires Sur, Servicio Nacional de Sanidad Y Calidad Agroalimentaria (SENASA), Mar del Plata, Argentina
Departamento de Química, Facultad de Ciencias Exactas Y Naturales, Universidad Nacional de Mar del Plata (UNMdP), Mar del Plata, Argentina
Palabras clave:calcium; multidrug resistance protein; placebo; steroid receptor; tamoxifen citrate; calcium; multidrug resistance protein; steroid receptor; tamoxifen; animal experiment; animal model; anthelmintic activity; article; body weight; calcium homeostasis; chemoprophylaxis; concentration (parameters); controlled study; drug efficacy; echinococcosis; Echinococcus granulosus; female; in vitro study; in vivo study; infection prevention; larval stage; metacestode; mouse; nonhuman; nucleotide sequence; parasite survival; priority journal; weight reduction; animal; antagonists and inhibitors; drug effects; echinococcosis; homeostasis; larva; metabolism; Animals; Calcium; Echinococcosis; Echinococcus granulosus; Female; Homeostasis; Larva; Mice; P-Glycoprotein; Receptors, Steroid; Tamoxifen
Año:2014
Volumen:58
Número:9
Página de inicio:5146
Página de fin:5154
DOI: http://dx.doi.org/10.1128/AAC.02113-13
Título revista:Antimicrobial Agents and Chemotherapy
Título revista abreviado:Antimicrob. Agents Chemother.
ISSN:00664804
CODEN:AMACC
CAS:calcium, 7440-70-2, 14092-94-5; multidrug resistance protein, 149200-37-3, 208997-77-7; tamoxifen citrate, 54965-24-1; tamoxifen, 10540-29-1; Calcium; P-Glycoprotein; Receptors, Steroid; Tamoxifen
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00664804_v58_n9_p5146_Nicolao

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Citas:

---------- APA ----------
Nicolao, M.C., Elissondo, M.C., Denegri, G.M., Goya, A.B. & Cumino, A.C. (2014) . In Vitro and in Vivo effects of tamoxifen against larval stage Echinococcus granulosus. Antimicrobial Agents and Chemotherapy, 58(9), 5146-5154.
http://dx.doi.org/10.1128/AAC.02113-13
---------- CHICAGO ----------
Nicolao, M.C., Elissondo, M.C., Denegri, G.M., Goya, A.B., Cumino, A.C. "In Vitro and in Vivo effects of tamoxifen against larval stage Echinococcus granulosus" . Antimicrobial Agents and Chemotherapy 58, no. 9 (2014) : 5146-5154.
http://dx.doi.org/10.1128/AAC.02113-13
---------- MLA ----------
Nicolao, M.C., Elissondo, M.C., Denegri, G.M., Goya, A.B., Cumino, A.C. "In Vitro and in Vivo effects of tamoxifen against larval stage Echinococcus granulosus" . Antimicrobial Agents and Chemotherapy, vol. 58, no. 9, 2014, pp. 5146-5154.
http://dx.doi.org/10.1128/AAC.02113-13
---------- VANCOUVER ----------
Nicolao, M.C., Elissondo, M.C., Denegri, G.M., Goya, A.B., Cumino, A.C. In Vitro and in Vivo effects of tamoxifen against larval stage Echinococcus granulosus. Antimicrob. Agents Chemother. 2014;58(9):5146-5154.
http://dx.doi.org/10.1128/AAC.02113-13