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Abstract:

In this work we describe the formation and microscopical application of a fluorescentderivative of Ruthenium Red (RR) obtained by heating the dye in the presence of 1,10-phenanthroline (OP). The RR-OP reaction product showed absorption maxima at 416 and 444 nm and intense fluorescence emission at 578 nm under 440 nm exciting light. Neither RR nor OP solutions alone were fluorescent when excited at 440 nm. Using fluorescence microscopy, chicken blood cell smears stained 5min with the RR-OP derivative showed the chromatin of erythrocyte nuclei with a bright orange fluorescence under violet-blue (436 nm) exciting light. © 1995, Gustav Fisher Verlag Jena GmbH. All rights reserved.

Registro:

Documento: Artículo
Título:Formation and microscopical application of a fluorescent 1,10-phenanthrolinederivative of Ruthenium Red
Autor:Bertolesi, G.E.; de Cidre, L.L.; Stockert, J.C.
Filiación:Cátedra de Histologia Animal, Departamento de Biología, Facultad de Ciencias Exactas y Naturales, y Area de Investigación, Instituto de Oncologia “A. H. Roffo”, Universidad de Buenos Aires, Argentina
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantobianco, Madrid, E-28049, Spain
Max-Planck-Institut für Biologie, Abteilung Mikrobiologie, Tübingen, D-72076, Germany
Palabras clave:1,10-phenanthroline; chromatin fluorescence; fluorescence microscopy; fluorescent ruthenium complexes; Ruthenium Red; 1,10 phenanthroline derivative; ruthenium red; ruthenium red derivative; unclassified drug; animal cell; article; chicken; controlled study; erythrocyte; fluorescence microscopy; nonhuman; technique; Animalia; Gallus gallus; Viola (angiosperm)
Año:1995
Volumen:97
Número:4
Página de inicio:401
Página de fin:408
DOI: http://dx.doi.org/10.1016/S0065-1281(11)80065-6
Título revista:Acta Histochemica
Título revista abreviado:Acta Histochem.
ISSN:00651281
CAS:ruthenium red, 11103-72-3, 1307-52-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00651281_v97_n4_p401_Bertolesi

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Citas:

---------- APA ----------
Bertolesi, G.E., de Cidre, L.L. & Stockert, J.C. (1995) . Formation and microscopical application of a fluorescent 1,10-phenanthrolinederivative of Ruthenium Red. Acta Histochemica, 97(4), 401-408.
http://dx.doi.org/10.1016/S0065-1281(11)80065-6
---------- CHICAGO ----------
Bertolesi, G.E., de Cidre, L.L., Stockert, J.C. "Formation and microscopical application of a fluorescent 1,10-phenanthrolinederivative of Ruthenium Red" . Acta Histochemica 97, no. 4 (1995) : 401-408.
http://dx.doi.org/10.1016/S0065-1281(11)80065-6
---------- MLA ----------
Bertolesi, G.E., de Cidre, L.L., Stockert, J.C. "Formation and microscopical application of a fluorescent 1,10-phenanthrolinederivative of Ruthenium Red" . Acta Histochemica, vol. 97, no. 4, 1995, pp. 401-408.
http://dx.doi.org/10.1016/S0065-1281(11)80065-6
---------- VANCOUVER ----------
Bertolesi, G.E., de Cidre, L.L., Stockert, J.C. Formation and microscopical application of a fluorescent 1,10-phenanthrolinederivative of Ruthenium Red. Acta Histochem. 1995;97(4):401-408.
http://dx.doi.org/10.1016/S0065-1281(11)80065-6