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Abstract:

Callichirus major, popularly known as ghost shrimp, is a species of great importance in the fishing industry, because of its use as live bait. This study aimed to describe the different stages of the developing ovaries in C. major. Shrimps were collected along the Corujão beach, Piuma, Brazil (20°50'41.6“S 40°44'15.7“W), and the gonads were dissected for histological and histochemical analysis. The ovary consists of two elongated filaments covered by a connective tissue that divides the organ into lobules, where somatic and germ cells are found. It was possible to classify five types of germ cells: Oogonia (Oog), previtellogenic oocyte (Oc1), early vitellogenic oocyte (Oc2), late vitellogenic oocyte (Oc3) and mature oocyte (Oc4) based on their vitellogenic stage, cytoplasmic, nuclear and morphometric characteristics. The histochemical analysis demonstrated an intense reaction for proteins and polysaccharides in peripheral cytoplasm of Oc3 comparing to others cell types. According to size, volume, color intensity and distribution of oocyte types the gonads were classified into: immature, developing, developed and spent, being in females at this last stage, observed empty follicles and oocytes in reabsorption process. During oogenesis was observed a gradual increase in cytoplasmic acidophilia due to accumulation of yolk granules and the intense histochemical reaction in periphery of Oc3, which indicate the beginning of an extravitellogenic source of nutrients. Based on the microscopic analysis of the vitellogenesis, shrimp C. major showed the initial short phase of oocyte growth following with a fast vitellogenic cycle. © 2017 Elsevier GmbH

Registro:

Documento: Artículo
Título:Dynamics of oogenesis in ghost shrimp Callichirus major (Crustacea: Axiidea): a morphofunctional and histochemical study
Autor:Souza, T.L.; Braga, A.A.; López-Greco, L.S.; Nunes, E.T.
Filiación:Departamento de Biologia − Centro de Ciências Exatas, Naturais e da Saúde − Universidade Federal do Espírito Santo, Alegre, Espírito Santo 29500-000, Brazil
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA, CONICET-UBA), Ciudad UniversitariaBuenos Aires C1428EHA, Argentina
Palabras clave:Ghost shrimp; Histochemistry; Oogenesis; Ovary; Reproduction; cell protein; polysaccharide; animal cell; animal structures; animal tissue; Article; Brazil; Callichirus major; cell nucleus; cell size; cell structure; cell volume; color; connective tissue; controlled study; cytoplasm; egg yolk; female; germ cell; histochemistry; histology; nonhuman; nutrient; oocyte; oocyte development; oogonium; ovary; ovary development; ovary follicle; shrimp; somatic cell; vitellogenesis; animal; Crustacea; cytology; metabolism; oocyte development; physiology; Animals; Crustacea; Female; Oocytes; Oogenesis; Ovary; Vitellogenesis
Año:2017
Volumen:119
Número:8
Página de inicio:769
Página de fin:777
DOI: http://dx.doi.org/10.1016/j.acthis.2017.09.001
Título revista:Acta Histochemica
Título revista abreviado:Acta Histochem.
ISSN:00651281
CODEN:AHISA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00651281_v119_n8_p769_Souza

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Citas:

---------- APA ----------
Souza, T.L., Braga, A.A., López-Greco, L.S. & Nunes, E.T. (2017) . Dynamics of oogenesis in ghost shrimp Callichirus major (Crustacea: Axiidea): a morphofunctional and histochemical study. Acta Histochemica, 119(8), 769-777.
http://dx.doi.org/10.1016/j.acthis.2017.09.001
---------- CHICAGO ----------
Souza, T.L., Braga, A.A., López-Greco, L.S., Nunes, E.T. "Dynamics of oogenesis in ghost shrimp Callichirus major (Crustacea: Axiidea): a morphofunctional and histochemical study" . Acta Histochemica 119, no. 8 (2017) : 769-777.
http://dx.doi.org/10.1016/j.acthis.2017.09.001
---------- MLA ----------
Souza, T.L., Braga, A.A., López-Greco, L.S., Nunes, E.T. "Dynamics of oogenesis in ghost shrimp Callichirus major (Crustacea: Axiidea): a morphofunctional and histochemical study" . Acta Histochemica, vol. 119, no. 8, 2017, pp. 769-777.
http://dx.doi.org/10.1016/j.acthis.2017.09.001
---------- VANCOUVER ----------
Souza, T.L., Braga, A.A., López-Greco, L.S., Nunes, E.T. Dynamics of oogenesis in ghost shrimp Callichirus major (Crustacea: Axiidea): a morphofunctional and histochemical study. Acta Histochem. 2017;119(8):769-777.
http://dx.doi.org/10.1016/j.acthis.2017.09.001