Artículo

Rivera-Ingraham, G.A.; Malanga, G.; Puntarulo, S.; Pérez, A.F.; Ruiz-Tabares, A.; Maestre, M.; González-Aranda, R.; Espinosa, F.; García-Gómez, J.C. "Antioxidant defenses and trace metal bioaccumulation capacity of cymbula nigra (Gastropoda: Patellidae)" (2013) Water, Air, and Soil Pollution. 224(3)
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Abstract:

The present study deals with the effect of trace metals on the endangered limpet Cymbula nigra. The Bay of Algeciras (Strait of Gibraltar) was used as the study site. Important industrial activity takes place in the area, including frequent oil spills. However, it is home to important populations of C. nigra. The objective of this work was to determine if these animals were being affected at a subcellular level by the pollutants present in their environment and to analyze the trace metal concentrations in the animal's soft tissues. To determine the effects of water quality on the antioxidant activity and concentrations through field experimentation, a total of six sites were selected in Algeciras Bay, three located in the inner areas (environmentally degraded sites with higher levels of pollutants) and three in the outermost areas of the Bay. Stress associated to reactive oxygen species formation was assessed on digestive glands and gills as the enzymatic antioxidant activity of catalase (CAT), superoxide dismutase (SOD), and glutathione S-transferase (GST) and as the concentrations of lipid-soluble (α-tocopherol and β-carotene) and the water-soluble antioxidants (reduced and oxidized glutathione (GSH and GSSG)). Gills and digestive glands of those animals located in the inner areas of Algeciras Bay showed higher CAT activity values than those located in the outer areas. As a general pattern, we observed higher antioxidant activities and concentrations in digestive glands that in gills, suggesting the possibility that pollutants are mainly being incorporated by limpets through the food. As a general rule, larger animals showed greater concentrations of these compounds. Iron, zinc, and manganese, in this order, were present in the tissues at the highest concentrations. Chromium and manganese were found in significantly higher concentrations in those animals collected from the inner areas of the Bay. Through the present study, we provide the first data regarding the antioxidant defense levels and metal accumulation capacity of this species, and we reinforce the idea that this endangered species may be, in fact, relatively tolerant to degraded environments. © 2013 Springer Science+Business Media Dordrecht.

Registro:

Documento: Artículo
Título:Antioxidant defenses and trace metal bioaccumulation capacity of cymbula nigra (Gastropoda: Patellidae)
Autor:Rivera-Ingraham, G.A.; Malanga, G.; Puntarulo, S.; Pérez, A.F.; Ruiz-Tabares, A.; Maestre, M.; González-Aranda, R.; Espinosa, F.; García-Gómez, J.C.
Filiación:Laboratorio de Biología Marina, Departamento de Fisiología y Zoología, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012 Sevilla, Spain
Functional Ecology, Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany
Fisicoquímica-IBIMOL Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, Buenos Aires, Argentina
Laboratorio de Ecología de Bentos Marino, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Cymbula; Limpet; ROS; Strait of Gibraltar; Subcellular stress; Trace metals; Cymbula; Limpet; ROS; Strait of Gibraltar; Sub-cellular; Trace metal; Agents; Conservation; Manganese; Occupational diseases; Oil spills; Oils and fats; Oxygen; Plants (botany); Tissue; Water pollution; Water quality; Animals; alpha tocopherol; beta carotene; catalase; chromium; glutathione; glutathione disulfide; glutathione transferase; iron; manganese; reactive oxygen metabolite; superoxide dismutase; trace metal; zinc; antioxidant; bioaccumulation; biological uptake; concentration (composition); endangered species; enzyme activity; experimental study; gastropod; industrial waste; oil spill; pollution effect; pollution exposure; pollution tolerance; trace metal; animal tissue; article; bioaccumulation; biological monitoring; chemical analysis; concentration (parameters); controlled study; Cymbula nigra; degradation kinetics; endangered species; enzyme activity; enzyme assay; exocrine gland; field experiment; food contamination; gastropod; geographical variation (species); gill; nonhuman; oil industry; oil spill; physical chemistry; species habitat; tissue distribution; water pollutant; water pollution indicator; water quality; Algeciras Bay; Andalucia; Cadiz [Andalucia]; Spain; Animalia; Cymbula; Gastropoda; Patellidae
Año:2013
Volumen:224
Número:3
DOI: http://dx.doi.org/10.1007/s11270-013-1458-8
Título revista:Water, Air, and Soil Pollution
Título revista abreviado:Water Air Soil Pollut.
ISSN:00496979
CODEN:WAPLA
CAS:alpha tocopherol, 1406-18-4, 1406-70-8, 52225-20-4, 58-95-7, 59-02-9; beta carotene, 7235-40-7; catalase, 9001-05-2; chromium, 14092-98-9, 16065-83-1, 7440-47-3; glutathione, 70-18-8; glutathione disulfide, 27025-41-8; glutathione transferase, 50812-37-8; iron, 14093-02-8, 53858-86-9, 7439-89-6; manganese, 16397-91-4, 7439-96-5; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; zinc, 14378-32-6, 7440-66-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00496979_v224_n3_p_RiveraIngraham

Referencias:

  • Aebi, H., Catalase in vitro (1984) Methods in Enzymology, 105, pp. 121-126. , 10.1016/S0076-6879(84)05016-3 1:CAS:528:DyaL2cXltVKis7s%3D
  • Alves De Almeida, E., Celso Dias Bainy, A., Paula De Melo Loureiro, A., Regina Martinez, G., Miyamoto, S., Onuki, J., Fujita Barbosa, L., Di Mascio, P., Oxidative stress in Perna perna and other bivalves as indicators of environmental stress in the Brazilian marine environment: Antioxidants, lipid peroxidation and DNA damage (2007) Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, 146 (4), pp. 588-600. , DOI 10.1016/j.cbpa.2006.02.040, PII S1095643306001644, Second Special Issue of CBP dedicated to "The Face of Latin American Comparative Biochemistry and Physiology"
  • Ansaldo, M., Najle, R., Luquet, C.M., Oxidative stress generated by diesel seawater contamination in the digestive gland of the Antarctic limpet Nacella concinna (2005) Marine Environmental Research, 59 (4), pp. 381-390. , DOI 10.1016/j.marenvres.2004.06.003, PII S0141113604002053
  • Boening, D.W., An evaluation of bivalves as biomonitors of heavy metals pollution in marine waters (1999) Environmental Monitoring and Assessment, 55 (3), pp. 459-470. , DOI 10.1023/A:1005995217901
  • Boyden, C.R., Zeldis, J.R., Preliminary observations using an attached microphonic sensor to study feeding behaviour of an intertidal limpet (1979) Estuarine Coastal and Marine Science, 9 (6), pp. 759-769. , 10.1016/S0302-3524(79)80009-2
  • Brown, R.J., Galloway, T.S., Lowe, D., Browne, M.A., Dissanayake, A., Jones, M.B., Depledge, M.H., Differential sensitivity of three marine invertebrates to copper assessed using multiple biomarkers (2004) Aquatic Toxicology, 66 (3), pp. 267-278. , DOI 10.1016/j.aquatox.2003.10.001
  • Bryan, G.W., Concentrations of zinc and copper in the tissues of decapod crustaceans (1968) Journal of the Marine Biological Association of the United Kingdom, 48, pp. 303-321. , 10.1017/S0025315400034500 1:CAS:528:DyaF1cXks1Gns7o%3D
  • Bryan, G.W., Langston, W.J., Hummerstone, L.G., Burt, G.R., (1985) A Guide to the Assessment of Heavy-metal Contamination Using Biological Indicators, , Occasional Publication of the Journal of the Marine Biological Association of the United Kingdom 4 Plymouth
  • Cadenas, E., Sies, H., Oxidative stress: Excited oxygen species and enzyme activity (1985) Advances in Enzyme Regulation, 23, pp. 217-237. , 10.1016/0065-2571(85)90049-4 1:CAS:528:DyaL2MXkvVemtb8%3D
  • Cajaraville, M.P., Bebianno, M.J., Blasco, J., Porte, C., Sarasquete, C., Viarengo, A., The use of biomarkers to assess the impact of pollution in coastal environments of the Iberian Peninsula: A practical approach (2000) Science of the Total Environment, 247 (2-3), pp. 295-311. , DOI 10.1016/S0048-9697(99)00499-4, PII S0048969799004994
  • Carballo, J.L., Naranjo, S.A., García-Gómez, J.C., Use of marine sponges as stress indicators in marine ecosystems at Algeciras Bay (southern Iberian Peninsula) (1996) Marine Ecology Progress Series, 135, pp. 109-122. , 10.3354/meps135109
  • Casas, S., Gonzalez, J.-L., Andral, B., Cossa, D., Relation between metal concentration in water and metal content of marine mussels (Mytilus galloprovincialis): Impact of physiology (2008) Environmental Toxicology and Chemistry, 27 (7), pp. 1543-1552. , DOI 10.1897/07-418.1
  • Catsiki, V.A., Bei, F., Nicolaidou, A., Size dependent metal concentrations in two marine gastropod species (1994) Neth.J.Aquat.Ecol., 28 (2), pp. 157-165
  • Cavaletto, M., Ghezzi, A., Burlando, B., Evangelisti, V., Ceratto, N., Viarengo, A., Effect of hydrogen peroxide on antioxidant enzymes and metallothionein level in the digestive gland of Mytilus galloprovincialis (2002) Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, 131 (4), pp. 447-455. , DOI 10.1016/S1532-0456(02)00030-3, PII S1532045602000303
  • Conradi, M., Lopez-Gonzalez, P.J., The benthic Gammaridea (Crustacea, Amphipoda) fauna of Algeciras Bay (Strait of Gibraltar): Distributional ecology and some biogeographical considerations (1999) Helgoland Marine Research, 53 (1), pp. 2-8
  • Conradi, M., López-González, P.J., García-Gómez, J.C., The amphipod community as a bioindicador in Algeciras Bay (Southern Iberian Peninsula) based on a spatio-temporal distribution (1997) Marine Ecology, 18, pp. 97-111. , 10.1111/j.1439-0485.1997.tb00430.x
  • Cravo, A., Bebianno, M.J., Bioaccumulation of metals in the soft tissue of Patella aspera: Application of metal/shell weight indices (2005) Estuarine, Coastal and Shelf Science, 65 (3), pp. 571-586. , DOI 10.1016/j.ecss.2005.06.026, PII S0272771405002428
  • Della Santina, P., Naylor, E., Endogenous rhythms in the homing behaviour of the limpet Patella vulgata Linnaeus (1993) Journal of Molluscan Studies, 59, pp. 87-91
  • Denton, G.R.W., Burdon-Jones, C., Influence of temperature and salinity on the uptake, distribution and depuration of mercury, cadmium and lead by the black-lip oyster Saccostrea echinata (1981) Marine Biology, 64 (3), pp. 317-326
  • Depledge, M.H., Rainbow, P.S., Models of regulation and accumulation of trace metals in marine invertebrates (1990) Comparative Biochemistry and Physiology, Part C, 97 (1), pp. 1-7. , 10.1016/0742-8413(90)90163-4
  • Depledge, M.H., Weeks, J.M., Bjerregaard, P., Heavy metals (1994) Handbook of Ecotoxicology, pp. 79-105. , Calow (eds) 2 Blackwell Oxford
  • Desai, I., Vitamin e analysis methods for animal tissues (1984) Methods in Enzymology, 105, pp. 138-146. , 10.1016/S0076-6879(84)05019-9 1:CAS:528:DyaL2cXksFWisrw%3D
  • Di Giulio, R.T., Washburn, P.C., Wenning, R.J., Winston, G.W., Jewell, C.S., Biochemical responses in aquatic animal: A review of determinants of oxidative stress (1989) Environmental Toxicology and Chemistry, 8 (12), pp. 1103-1123. , 10.1002/etc.5620081203
  • Douhri, H., Sayah, F., The use of enzymatic biomarkers in two marine invertebrates Nereis diversicolor and Patella vulgata for the biomonitoring of Tangier's bay (Morocco) (2009) Ecotoxicology and Environmental Safety, 72 (2), pp. 394-399. , 10.1016/j.ecoenv.2008.07.016 1:CAS:528:DC%2BD1cXhtlaqtbfO
  • Doyotte, A., Cossu, C., Jacquin, M.-C., Babut, M., Vasseur, P., Antioxidant enzymes, glutathione and lipid peroxidation as relevant biomarkers of experimental or field exposure in the gills and the digestive gland of the freshwater bivalve Unio tumidus (1997) Aquatic Toxicology, 39 (2), pp. 93-110. , DOI 10.1016/S0166-445X(97)00024-6, PII S0166445X97000246
  • Dwivedi, S., Tripathi, R.D., Rai, U.N., Srivastava, S., Mishra, S., Shukla, M.K., Gupta, A.K., Gupta, D.K., Dominance of algae in ganga water polluted through fly-ash leaching: Metal bioaccumulation potential of selected algal species (2006) Bulletin of Environmental Contamination and Toxicology, 77 (3), pp. 427-436. , DOI 10.1007/s00128-006-1083-y
  • Eaton, D.L., Bammler, T.K., Concise review of the glutathione S-transferases and their significance to toxicology (1999) Toxicological Sciences, 49 (2), pp. 156-164. , DOI 10.1093/toxsci/49.2.156
  • Espinosa, F., Guerra-Garcia, J.M., Garcia-Gomez, J.C., Sewage pollution and extinction risk: An endangered limpet as a bioindicator? (2007) Biodiversity and Conservation, 16 (2), pp. 377-397. , DOI 10.1007/s10531-005-3014-3
  • Espinosa, F., Nakano, T., Guerra-García, J.M., García- Gómez, J.C., Population genetic structure of the endangered limpet Cymbula nigra in a temperate Northern hemisphere region: Influence of paleoclimatic events? (2011) Marine Ecology, 32 (1), pp. 1-5. , 10.1111/j.1439-0485.2010.00410.x
  • Farombi, E.O., Adelowo, O.A., Ajimoko, Y.R., Biomarkers of oxidative stress and heavy metal levels as indicators of environmental pollution in African cat fish (Clarias gariepinus) from Nigeria Ogun River (2007) International Journal of Environmental Research and Public Health, 4 (2), pp. 158-165. , http://www.mdpi.org/ijerph/papers/ijerph2007040011.pdf
  • Feldstein, T., Kashman, Y., Abelson, A., Fishelson, L., Mokady, O., Bresler, V., Erel, Y., Marine molluscs in environmental monitoring III. Trace metals and organic pollutants in animal tissue and sediments (2003) Helgoland Marine Research, 57 (3-4), pp. 212-219. , DOI 10.1007/s10152-003-0156-0
  • Fenton, H.J.H., Oxidation of tartaric acid in presence of iron (1894) Journal of the Chemical Society, 65, pp. 899-910. , 10.1039/ct8946500899 1:CAS:528:DyaD28XmtlCnsQ%3D%3D
  • Fernández, B., Campillo, J.A., Martínez-Gómez, C., Benedicto, J., Antioxidant responses in gills of mussel (Mytilus galloprovincialis) as biomarkers of environmental stress along the Spanish Mediterranean coast (2010) Aquatic Toxicology, 99 (2), pp. 186-197. , 10.1016/j.aquatox.2010.04.013
  • Flohe, L., Otting, F., Superoxide dismutase assays (1984) Methods in Enzymology, VOL. 105, pp. 93-104
  • Frazier, J.M., George, S.S., Overnell, J., Characterization of two molecular weight classes of cadmium binding proteins from the mussel, Mytilus edulis (L.) (1985) Comparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology, 80 (2), pp. 257-262
  • Frenkiel, L., Contribution a l'étude des cycles de reproduction des Patellidae en Algérie (1975) Pubblicacione de la Stazzione Zoologica di Napoli, 39, pp. 153-189
  • Guerra-Garcia, J.M., Maestre, M.J., Gonzalez, A.R., Garcia-Gomez, J.C., Assessing a quick monitoring method using rocky intertidal communities as a bioindicator: A multivariate approach in Algeciras Bay (2006) Environmental Monitoring and Assessment, 116 (1-3), pp. 345-361. , DOI 10.1007/s10661-006-7572-y
  • Guerra-García, J.M., Ruiz-Tabares, A., Baeza-Rojano, E., Cabezas, M.P., Díaz-Pavón, J.J., Pacios, I., Trace metals in Caprella (Crustacea: Amphipoda). A new tool for monitoring pollution in coastal areas? (2010) Biological Indicators, 10 (3), pp. 734-743. , 10.1016/j.ecolind.2009.12.003
  • Haber, F., Weiss, J., Über die katalyse des hydroperoxydes (1932) Naturwissenschaften, 20 (51), pp. 948-950. , 10.1007/BF01504715 1:CAS:528:DyaA3sXhslWktg%3D%3D
  • Habig, W.H., Pabst, M.J., Jakoby, W.B., Glutathione S-transferases: The first enzymatic step in mercapturic acid formation (1974) Journal of Biological Chemistry, 249 (22), pp. 7130-7139. , 1:CAS:528:DyaE2MXltlahtg%3D%3D
  • Hansen, J.A., Welsh, P.G., Lipton, J., Suedkamp, M.J., The effects of long-term cadmium exposure on the growth and survival of juvenile bull trout (Salvelinus confluentus) (2002) Aquatic Toxicology, 58 (3-4), pp. 165-174. , DOI 10.1016/S0166-445X(01)00233-8, PII S0166445X01002338
  • Harada, H., Kurauchi, M., Hayashi, R., Eki, T., Shortened lifespan of nematode Caenorhabditis elegans after prolonged exposure to heavy metals and detergents (2007) Ecotoxicology and Environmental Safety, 66 (3), pp. 378-383. , DOI 10.1016/j.ecoenv.2006.02.017, PII S0147651306000583
  • Hayashi, M., Ueda, T., Uyeno, K., Wada, K., Kinae, N., Saotome, K., Tanaka, N., Ojima, Y., Development of genotoxicity assay systems that use aquatic organisms (1998) Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 399 (2), pp. 125-133. , DOI 10.1016/S0027-5107(97)00251-0, PII S0027510797002510
  • Hutcheson, M.S., The effect of temperature and salinity on cadmium uptake by the blue crab, Callinectes sapidus (1974) Chesapeake Science, 15 (4), pp. 237-241. , 10.2307/1350976 1:CAS:528:DyaE28XlsV2nsA%3D%3D
  • Karr, J.R., Dudley, D.R., Ecological perspective on water quality goals (1981) Environmental Management, 5 (1), pp. 55-68. , DOI 10.1007/BF01866609
  • Koufopanou, V., Reid, D.G., Ridgway, S.A., Thomas, R.H., A Molecular Phylogeny of the Patellid Limpets (Gastropoda: Patellidae) and Its Implications for the Origins of Their Antitropical Distribution (1999) Molecular Phylogenetics and Evolution, 11 (1), pp. 138-156. , DOI 10.1006/mpev.1998.0557, PII S1055790398905577
  • Krantzberg, G., Metal accumulation by chironomid larvae: The effects of age and body weight on metal body burdens (1989) Hydrobiologia, 188-189, pp. 497-506. , 10.1007/BF00027817
  • Lance, E., Neffling, M.R., Gérard, C., Meriluoto, J., Bormans, M., Accumulation of free and covalently bound microcystins in tissues of Lymnaea stagnalis (Gastropoda) following toxic cyanobacteria or dissolved microcystic-LR exposure (2010) Environmental Pollution, 158 (3), pp. 674-680. , 10.1016/j.envpol.2009.10.025 1:CAS:528:DC%2BC3cXot1Ontg%3D%3D
  • Langston, W.J., Bebianno, M.J., Burt, G.R., Metal handling strategies in molluscs (1998) Metal Metabolism in Aquatic Environments, pp. 219-283. , W.J. Langston M.J. Bebianno (eds) Chapman and Hall London
  • Lares, M.L., Orians, K.J., Natural Cd and Pb variations in Mytilus californianus during the upwelling season (1997) Science of the Total Environment, 197 (1-3), pp. 177-195. , DOI 10.1016/S0048-9697(96)05409-5, PII S0048969796054095
  • Leaver, M.J., George, S.G., A piscine glutathione S-transferase which efficiently conjugates the end-products of lipid peroxidation (1998) Marine Environmental Research, 46 (1-5), pp. 71-74. , DOI 10.1016/S0141-1136(97)00071-8, PII S0141113697000718
  • Lionetto, M.G., Caricato, R., Giordano, M.E., Pascariello, M.F., Marinosci, L., Schettino, T., Integrated use of biomarkers (acetylcholinesterase and antioxidant enzymes activities) in Mytilus galloprovincialis and Mullus barbatus in an Italian coastal marine area (2003) Marine Pollution Bulletin, 46 (3), pp. 324-330. , DOI 10.1016/S0025-326X(02)00403-4
  • Livingstone, D.R., Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms (2001) Marine Pollution Bulletin, 42 (8), pp. 656-666. , DOI 10.1016/S0025-326X(01)00060-1, PII S0025326X01000601
  • Livingstone, D.R., Förlin, L., George, S.G., Molecular biomarkers and toxic consequences of impacts by organic pollution in aquatic organisms (1994) Water Quality and Stress Indicators in Marine and Freshwater Systems: Linking Levels of Organization, pp. 154-171. , D.W. Sutcliffe (eds) Freshwater Biological Association Ambleside, United Kingdom
  • Lowry, O.M., Rosenbrough, N.J., Farr, O.L., Randall, R.J., Protein measurement with the folin phenol reagent (1951) Journal of Biological Chemistry, 193, pp. 265-275. , 1:CAS:528:DyaG38XhsVyrsw%3D%3D
  • Malanga, G., Estevez, M.S., Calvo, J., Abele, D., Puntarulo, S., The effect of seasonality on oxidative metabolism in Nacella (Patinigera) magellanica (2007) Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, 146 (4), pp. 551-558. , DOI 10.1016/j.cbpa.2006.01.029, PII S1095643306000535, Second Special Issue of CBP dedicated to "The Face of Latin American Comparative Biochemistry and Physiology"
  • Manna, G.K., Sadhukhan, A., Use of cells of gill and kidney of tilapia fish in micronucleus test (MNT) (1986) Current Science, 55, pp. 498-501
  • McCord, J.M., Fridovich, I., Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein) (1969) Journal of Biological Chemistry, 244, pp. 6049-6055. , 1:CAS:528:DyaE3cXmsVU%3D
  • Miller III, E.R., Pondick, J.S., Heavy metal levels in Nucella lapillus (Gastropoda: Prosobranchia) from sites with normal and penis-bearing females from New England (1984) Bulletin of Environmental Contamination and Toxicology, 33 (5), pp. 612-620
  • Morales-Caselles, C., Kalman, J., Riba, I., DelValls, T.A., Comparing sediment quality in Spanish littoral areas affected by acute (Prestige, 2002) and chronic (Bay of Algeciras) oil spills (2007) Environmental Pollution, 146 (1), pp. 233-240. , DOI 10.1016/j.envpol.2006.04.042, PII S0269749106005483
  • Morillo, J., Usero, J., Trace metal bioavailability in the waters of two different habitats in Spain: Huelva estuary and Algeciras Bay (2008) Ecotoxicology and Environmental Safety, 71 (3), pp. 851-859. , 10.1016/j.ecoenv.2008.01.016 1:CAS:528:DC%2BD1cXht1Sit7%2FJ
  • Nakhlé, K.F., Cossa, D., Khalaf, G., Beliaeff, B., Brachidontes variabilis and Patella sp. as quantitative biological indicators for cadmium, lead and mercury in the Lebanese coastal waters (2006) Environmental Pollution, 142 (1), pp. 73-82. , 10.1016/j.envpol.2005.09.016
  • Navrot, J., Amiel, A.J., Kronfeld, J., Patella vulgata: A biological monitor of coastal metal pollution - A preliminary study (1974) Environmental Pollution, 7 (4), pp. 303-308. , 10.1016/0013-9327(74)90038-X 1:CAS:528:DyaE2MXhtF2ktLw%3D
  • Nias, D.J., McKillup, S.C., Edyvane, K.S., Imposex in Lepsiella vinosa from Southern Australia (1993) Marine Pollution Bulletin, 26 (7), pp. 380-384. , 10.1016/0025-326X(93)90185-M 10.1016/0025-326X(93)90185-M 1:CAS:528:DyaK2cXht1OgsL4%3D
  • Niyogi, S., Biswas, S., Sarker, S., Datta, A.G., Antioxidant enzymes in brackishwater oyster, Saccostrea cucullata as potential biomarkers of polyaromatic hydrocarbon pollution in Hooghly Estuary (India): Seasonality and its consequences (2001) Science of the Total Environment, 281 (1-3), pp. 237-246. , DOI 10.1016/S0048-9697(01)00850-6, PII S0048969701008506
  • Orren, M.J., Eagle, G.A., Hennig, H.F.K.O., Green, A., Variations in trace metal content of the mussel Choromytilus meridionalis (Kr.) with season and sex (1980) Marine Pollution Bulletin, 11 (9), pp. 253-257. , DOI 10.1016/0025-326X(80)90313-6
  • Peterson, R.C.J., Population and guild analysis for interpretation of heavy metal pollution in streams (1986) Community Toxicity Testing, pp. 180-198. , J. Cairns Jr (eds) Special Technical Publications 920 American Society for Testing and Materials Philadelphia 10.1520/STP23056S
  • Phillips, D.J.H., The common mussel Mytilus edulis as an indicator of pollution by zinc, cadmium, lead and copper. I. Effects of environmental variables on uptake of metals (1976) Marine Biology, 37 (2), pp. 59-69
  • Phillips, D.J.H., The use of biological indicator organisms to monitor trace metal pollution in marine and estuarine environments: A review (1977) Environmental Pollution, 13 (4), pp. 281-317
  • Rainbow, P.S., Trace metal accumulation in marine invertebrates: Marine biology or marine chemistry? (1997) Journal of the Marine Biological Association of the United Kingdom, 77 (1), pp. 195-210. , 10.1017/S0025315400033877 1:CAS:528:DyaK2sXitVaqtbk%3D
  • Regoli, F., Principato, G., Glutathione, glutathione-dependent and antioxidant enzymes in mussel, Mytilus galloprovincialis, exposed to metals under field and laboratory conditions: Implications for the use of biochemical biomarkers (1995) Aquatic Toxicology, 31 (2), pp. 143-164. , 10.1016/0166-445X(94)00064-W 10.1016/0166-445X(94)00064-W 1:CAS:528:DyaK2MXjvVensL8%3D
  • Regoli, F., Gorbi, S., Frenzilli, G., Nigro, M., Corsi, I., Focardi, S., Winston, G.W., Oxidative stress in ecotoxicology: From the analysis of individual antioxidants to a more integrated approach (2002) Marine Environmental Research, 54 (3-5), pp. 419-423. , DOI 10.1016/S0141-1136(02)00146-0, PII S0141113602001460
  • Regoli, F., Nigro, M., Chiantore, M., Winston, G.W., Seasonal variations of susceptibility to oxidative stress in Adamussium colbecki, a key bioindicator species for the Antarctic marine environment (2002) Science of the Total Environment, 289 (1-3), pp. 205-211. , DOI 10.1016/S0048-9697(01)01047-6, PII S0048969701010476
  • Renault, L., Moueza, M., Contribution à l'étude de Patella safiana Lamarck (1971) Haliotis, 1, pp. 19-20
  • Ridgway, S.A., Reid, D.G., Taylor, J.D., Branch, G.M., Hodgson, A.N., A cladistic phylogeny of the family Patellidae (Mollusca: Gastropoda) (1998) Philosophical Transactions of the Royal Society B: Biological Sciences, 353, pp. 1645-1671. , 10.1098/rstb.1998.0316
  • Rivera-Ingraham, G.A., (2010) Biología de la Conservación de Especies de Patélidos en El Umbral Atlántico-Mediterráneo, , Tesis Doctoral. Universidad de Sevilla Sevilla, Spain
  • Rivera-Ingraham, G.A., Espinosa, F., García-Gómez, J.C., Ecological considerations and niche differentiation between juvenile and adult black limpets (Cymbula nigra) (2011) Journal of the Marine Biological Association of the United Kingdom, 91 (1), pp. 191-198. , 10.1017/S0025315410000159
  • Rodriguez-Ariza, A., Toribio, F., Lopez-Barea, J., Rapid determination of glutathione status in fish liver using high-performance liquid chromatography and electrochemical detection (1994) Journal of Chromatography B: Biomedical Applications, 656 (2), pp. 311-318. , DOI 10.1016/0378-4347(94)00111-1
  • Sánchez-Moyano, E., (1996) Variación Espacio-temporal en la Composición de Las Comunidades Animales Asociadas A Macroalgas Como Respuestas A Cambios en El Medio. Implicaciones en la Caracterización Ambiental de Las Áreas Costeras, , University of Sevilla Sevilla, Spain
  • Sa-Pinto, A., Branco, M., Harris, D.J., Alexandrino, P., Phylogeny and phylogeography of the genus Patella based on mitochondrial DNA sequence data (2005) Journal of Experimental Marine Biology and Ecology, 325 (1), pp. 95-110. , DOI 10.1016/j.jembe.2005.04.025, PII S0022098105002248
  • Sies, H., Oxidative stress: From basic research to clinical application (1991) The American Journal of Medicine, 91 (3), pp. 31-S38. , 10.1016/0002-9343(91)90281-2
  • Stegeman, J.J., Brouwner, M., Giulio, R.T.D., Förlin, L., Fowler, B.A., Sanders, B.M., Molecular responses to environmental contamination: Enzyme and protein systems as indicators of chemical exposure and effect (1992) Biomarkers, Biochemical, Physiological, and Histological Markers of Anthropogenic Stress, pp. 253-336. , R.J. Huggett R.A. Kimerle M. Mehrle Jr H.L. Bergman (eds) Lewis Publishers United States of America
  • Valavanidis, A., Vlahogianni, T., Dassenakis, M., Scoullos, M., Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants (2006) Ecotoxicology and Environmental Safety, 64 (2), pp. 178-189. , DOI 10.1016/j.ecoenv.2005.03.013, PII S0147651305000643
  • Vasconcelos, V.M., Uptake and depuration of the heptapeptide toxin microcystin-LR in Mytilus galloprovincialis (1995) Aquatic Toxicology, 32 (2-3), pp. 227-237. , 10.1016/0166-445X(94)00085-5 1:CAS:528:DyaK2MXmtVygu7o%3D
  • Vasconcelos, V., Oliveira, S., Teles, F.O., Impact of a toxic and a non-toxic strain of Microcystis aeruginosa on the crayfish Procambarus clarkii (2001) Toxicon, 39 (10), pp. 1461-1470. , DOI 10.1016/S0041-0101(01)00105-2, PII S0041010101001052
  • Viarengo, A., Heavy metals in marine invertebrates: Mechanisms of regulation and toxicity at the cellular level (1989) CRC Review of Aquatic Sciences, 1, pp. 295-317. , 1:CAS:528:DyaL1MXkvFWksb0%3D
  • Vlahogianni, T., Dassenakis, M., Scoullos, M.J., Valavanidis, A., Integrated use of biomarkers (superoxide dismutase, catalase and lipid peroxidation) in mussels Mytilus galloprovincialis for assessing heavy metals' pollution in coastal areas from Saronikos Gulf of Greece (2007) Marine Pollution Bulletin, 54 (9), pp. 1361-1371. , 10.1016/j.marpolbul.2007.05.018 1:CAS:528:DC%2BD2sXhtVCmurvP
  • Vosyliene, M.Z., Kazlauskiene, N., Svecevicius, G., Effect of a heavy metal model mixture on biological parameters of rainbow trout Oncorhynchus mykiss (2003) Environmental Science and Pollution Research, 10 (2), pp. 103-107
  • Weihe, E., Kriews, M., Abele, D., Differences in heavy metal concentrations and in the response of the antioxidant system to hypoxia and air exposure in the Antarctic limpet Nacella concinna (2010) Marine Environmental Research, 69 (3), pp. 127-135. , 10.1016/j.marenvres.2009.09.003 1:CAS:528:DC%2BC3cXhs1yiuro%3D
  • Winston, G.W., Di Giulio, R.T., Prooxidant and antioxidant mechanisms in aquatic organisms (1991) Aquatic Toxicology, 19 (2), pp. 137-161. , 10.1016/0166-445X(91)90033-6 1:CAS:528:DyaK3MXks1Kgsrc%3D
  • Yoshinaga, M., Ueki, T., Michibata, H., Metal binding ability of glutathione transferases conserved between two animal species, the vanadium-rich ascidian Ascidia sydneiensis samea and the schistosome Schistosoma japonicum (2007) Biochimica et Biophysica Acta - General Subjects, 1770 (9), pp. 1413-1418. , DOI 10.1016/j.bbagen.2007.05.007, PII S0304416507001195

Citas:

---------- APA ----------
Rivera-Ingraham, G.A., Malanga, G., Puntarulo, S., Pérez, A.F., Ruiz-Tabares, A., Maestre, M., González-Aranda, R.,..., García-Gómez, J.C. (2013) . Antioxidant defenses and trace metal bioaccumulation capacity of cymbula nigra (Gastropoda: Patellidae). Water, Air, and Soil Pollution, 224(3).
http://dx.doi.org/10.1007/s11270-013-1458-8
---------- CHICAGO ----------
Rivera-Ingraham, G.A., Malanga, G., Puntarulo, S., Pérez, A.F., Ruiz-Tabares, A., Maestre, M., et al. "Antioxidant defenses and trace metal bioaccumulation capacity of cymbula nigra (Gastropoda: Patellidae)" . Water, Air, and Soil Pollution 224, no. 3 (2013).
http://dx.doi.org/10.1007/s11270-013-1458-8
---------- MLA ----------
Rivera-Ingraham, G.A., Malanga, G., Puntarulo, S., Pérez, A.F., Ruiz-Tabares, A., Maestre, M., et al. "Antioxidant defenses and trace metal bioaccumulation capacity of cymbula nigra (Gastropoda: Patellidae)" . Water, Air, and Soil Pollution, vol. 224, no. 3, 2013.
http://dx.doi.org/10.1007/s11270-013-1458-8
---------- VANCOUVER ----------
Rivera-Ingraham, G.A., Malanga, G., Puntarulo, S., Pérez, A.F., Ruiz-Tabares, A., Maestre, M., et al. Antioxidant defenses and trace metal bioaccumulation capacity of cymbula nigra (Gastropoda: Patellidae). Water Air Soil Pollut. 2013;224(3).
http://dx.doi.org/10.1007/s11270-013-1458-8