Artículo
Abstract:
A systematic study of the role of the Ca2+ and Mn2+ ions on the interaction between heparin and Concanavalin A was carried out. The protein was demetallized, and complex formation was followed by a light scattering assay. In the absence of ions no visible interaction was detected. Maximum activity was obtained when Mn2+ ions were added. A similar effect was observed when Ca2+ ions alone were used. Kinetics of the interaction in the presence of optimal Mn2+ and (or) Ca2+ concentrations confirmed the role of Ca2+ in accelerating a conformational change leading to a functional protein. A peculiar effect of activation-inhibition depending on ion concentration was also exhibited by Ca2+. These results confirm that Ca2+ and Mn2+ can occupy both sites on Concanavalin A and activate the protein for binding heparin. They point also to a crucial role of Ca2+ in the binding capacity of the active heparin fraction.
Registro:
| Documento: | Artículo |
| Título: | Heparin and Concanavalin A interaction as a model for studying the mechanism of the anticoagulant activity |
| Autor: | Monge, J.C.; Recondo, E.F.; Fernandez de Recondo, M.E. |
| Filiación: | Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina |
| Idioma: | Inglés |
| Palabras clave: | calcium; concanavalin a; heparin; magnesium; anticoagulation; methodology; model; nonbiological model; nonhuman; priority journal; Binding, Competitive; Blood Coagulation; Calcium; Concanavalin A; Drug Interactions; Heparin; Kinetics; Light; Manganese; Models, Biological; Protein Conformation; Scattering, Radiation; Spectrophotometry; Support, Non-U.S. Gov't |
| Año: | 1989 |
| Volumen: | 54 |
| Número: | 3 |
| Página de inicio: | 237 |
| Página de fin: | 243 |
| Título revista: | Thrombosis Research |
| Título revista abreviado: | THROMB. RES. |
| ISSN: | 00493848 |
| CODEN: | THBRA |
| CAS: | calcium, 7440-70-2; concanavalin A, 11028-71-0; heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; magnesium, 7439-95-4; Calcium, 7440-70-2; Concanavalin A, 11028-71-0; Heparin, 9005-49-6; Manganese, 7439-96-5 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00493848_v54_n3_p237_Monge |
Citas:
---------- APA ----------
Monge, J.C., Recondo, E.F. & Fernandez de Recondo, M.E. (1989) . Heparin and Concanavalin A interaction as a model for studying the mechanism of the anticoagulant activity. Thrombosis Research, 54(3), 237-243.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00493848_v54_n3_p237_Monge [ ]
---------- CHICAGO ----------
Monge, J.C., Recondo, E.F., Fernandez de Recondo, M.E. "Heparin and Concanavalin A interaction as a model for studying the mechanism of the anticoagulant activity" . Thrombosis Research 54, no. 3 (1989) : 237-243.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00493848_v54_n3_p237_Monge [ ]
---------- MLA ----------
Monge, J.C., Recondo, E.F., Fernandez de Recondo, M.E. "Heparin and Concanavalin A interaction as a model for studying the mechanism of the anticoagulant activity" . Thrombosis Research, vol. 54, no. 3, 1989, pp. 237-243.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00493848_v54_n3_p237_Monge [ ]
---------- VANCOUVER ----------
Monge, J.C., Recondo, E.F., Fernandez de Recondo, M.E. Heparin and Concanavalin A interaction as a model for studying the mechanism of the anticoagulant activity. THROMB. RES. 1989;54(3):237-243.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00493848_v54_n3_p237_Monge [ ]
