Abstract:
Plasma from 7 septic patients with positive blood cultures were studied. None of them presented either clinical or laboratory evidence of Disseminated Intravascular Coagulation. The white cells count varied between 5 and 45×109/l. In plasma functional plasminogen levels varied between 25 and 45%, while those of α2-antiplasmin were normal (80-105%). The levels of elastase ranged between 250 and 750 ug/ml. Leukocyte elastase digests plasminogen "in vitro" and is able to produce several fragments; one of them called mini-plasminogen lacking lysine binding sites; therefore it does not bind to lysine-Sepharose 4B. Two different behaviors were observed in the plasmatic plasminogen of these patients with respect to their binding capacity to lysine-Sepharose 4 B. 3 patients had plasminogen which did not bind to lysine-Sepharose 4 B; the other 4 had two different components, one of which bound to lysine-Sepharose 4 B and another one which did not bind. Previous studies "in vitro" have shown that leukocyte elastase modifies α2-antiplasmin, initially producing a non-plasminogen binding form. A free α2-antiplasmin (non-plasminogen binding form) was detected in the plasma of these patients with sepsis by crossed immunoelectrophoresis with plasminogen in the first dimension. It seems tenable that high levels of leukocyte elastase could be responsible for these findings although, the possible relationships to leukocyte elastase still remain to be proven but could possibly explain this effect. © 1987.
Registro:
Documento: |
Artículo
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Título: | Mini-plasminogen like molecule in septic patients |
Autor: | Kordich, L.C.; Porterie, V.P.; Lago, O.; Bergonzelli, G.E.; Sassetti, B.; Sanchez Avalos, J.C. |
Filiación: | Departament of Biological Chemistry. Facultad de Ciencias Exactas, Naturales. Universidad de Buenos Aires, Argentina Departament of Hematology. Hospital Privado GUemes, Buenos Aires, Argentina
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Palabras clave: | mini-plasminogen; sepsis; α2-antiplasmin; alpha 2 antiplasmin; elastase; miniplasminogen; unclassified drug; article; blood and hemopoietic system; etiology; human; human cell; leukocyte; sepsis; alpha 1-Antitrypsin; Antiplasmin; Blood Proteins; Humans; Leukocytes; Pancreatic Elastase; Plasminogen; Sepsis |
Año: | 1987
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Volumen: | 47
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Número: | 5
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Página de inicio: | 553
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Página de fin: | 560
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DOI: |
http://dx.doi.org/10.1016/0049-3848(87)90360-4 |
Título revista: | Thrombosis Research
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Título revista abreviado: | Thromb. Res.
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ISSN: | 00493848
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CODEN: | THBRA
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CAS: | elastase, 9004-06-2; Antiplasmin; Blood Proteins; Pancreatic Elastase, 3.4.21.36; Plasminogen, 9001-91-6; alpha 1-Antitrypsin
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00493848_v47_n5_p553_Kordich |
Referencias:
- Collen, On the Regulation and Control of Fibrinolysis (1980) Thromb. Haemostas., 43, pp. 77-89
- Kordich, Sassetti, Lago, Hemostasia en leucemias agudas no tratadas (1980) Sangre (Zaragoza), 25, pp. 1022-1033
- Kordich, Sassetti, Lago, Hemostasia en leucemias agudas no tratadas. II-Inhibidores Naturales: Actividad Biologica e immunologica - Variacion inmunologica de Antitrombina III (1984) Sangre (Zaragoza), 29, pp. 133-140
- Egbring, Schmidt, Fuchs, Havemann, Demonstration of granulocytic proteases in plasma of patients with acute leukemia and septicemia with coagulation defects (1977) Blood, 58, pp. 219-231
- Schmidt, Egbring, Havemann, Effect of elastase - like neutral protease from human granulocytes on isolated clotting factors (1974) Thrombosis Research, 6, pp. 315-326
- Moroz, Mini - Plasminogen A mechanism for leukocyte modulation of Plasminogen Activation by Urokinase (1981) Blood, 58, pp. 97-104
- Gramse, Egbring, Havemann, α2-Plasmin Inhibitor Inactivation by Human Leukocyte Elastase (1984) Hoppe-Seyler´s Zeitschrift für physiologische Chemie, 365, pp. 19-26
- Kordich, Porterie, Barazzutti, Nakkache, Korin, Sanchez Avalos, Letter to The Editors-In-Chief (1986) Thrombosis Research, 44 (6), p. 899
- Soria, Soria, Samama, Dosage du plasminogéne a l'aide d'un substract chromogene tripeptidique (1976) Pathol. Biol. Fran., 10 (24), pp. 725-729
- Laurell, Quantitative estimation of proteins by electrophoresis in agarose containing antibodies (1986) Analytical Biochemistry, 15, pp. 45-52
- Kordich, Feldman, Porterie, Lago, Severe Hemorrhagic Tendency in Heterozygous α2-Antiplasmin Deficiency (1985) Thrombosis Research, 40, pp. 645-651
- Deutsch, Mertz, Plasminogen purification from Human Plasma by Affinity Chromatography (1970) Science, 170, pp. 1095-1096
- Fenton, Fasco, Stackrow, Aronson, Young, Finlayson, Human Thrombins (1977) Production, evaluation and properties of α-Thrombin, 252, pp. 3587-3598. , IInd ed., J. Biol.Chem., (11)
- Matsuda, Wakabayashi, Aoki, Morioka, α2-Plasmin Inhibitor is among acute phase reactants (1980) Thrombosis Research, 17, pp. 527-530
- Teger, Nillson, Gyzander, Myrwood, Noppa, Olsson, Wallmo, Determination of fast - acting inhibitor ( α2-antiplasmin) in plasma from patients with tendency to thrombosis and increased fibrinolysis (1978) Haesmostasis, 7, pp. 155-157
- Kordich, L., Porterie, P., Tartas, N., Sanchez Avalos, J.C., Unpublished Results
Citas:
---------- APA ----------
Kordich, L.C., Porterie, V.P., Lago, O., Bergonzelli, G.E., Sassetti, B. & Sanchez Avalos, J.C.
(1987)
. Mini-plasminogen like molecule in septic patients. Thrombosis Research, 47(5), 553-560.
http://dx.doi.org/10.1016/0049-3848(87)90360-4---------- CHICAGO ----------
Kordich, L.C., Porterie, V.P., Lago, O., Bergonzelli, G.E., Sassetti, B., Sanchez Avalos, J.C.
"Mini-plasminogen like molecule in septic patients"
. Thrombosis Research 47, no. 5
(1987) : 553-560.
http://dx.doi.org/10.1016/0049-3848(87)90360-4---------- MLA ----------
Kordich, L.C., Porterie, V.P., Lago, O., Bergonzelli, G.E., Sassetti, B., Sanchez Avalos, J.C.
"Mini-plasminogen like molecule in septic patients"
. Thrombosis Research, vol. 47, no. 5, 1987, pp. 553-560.
http://dx.doi.org/10.1016/0049-3848(87)90360-4---------- VANCOUVER ----------
Kordich, L.C., Porterie, V.P., Lago, O., Bergonzelli, G.E., Sassetti, B., Sanchez Avalos, J.C. Mini-plasminogen like molecule in septic patients. Thromb. Res. 1987;47(5):553-560.
http://dx.doi.org/10.1016/0049-3848(87)90360-4