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Abstract:

Dermatan sulfate (DS) is a member of the family of structurally complex, sulfated, linear heteropolysaccharides called glycosaminoglycans (GAGs). It has a similar structure to heparin and heparan sulfate (HS), but with acetylgalactosamine replacing glucosamine, and the uronic acid moiety, mainly iduronic, joined 1→3 to the hexosamine. We are studying the relationships between structure and activities of dermatan sulfate, in particular those associated with the thrombin inhibition mediated by heparin cofactor II (HCII). As we have demonstrated with heparin, a small fraction of dermatan sulfate was isolated by precipitation with the first component of the complement system, under very specific conditions of low ionic strength, and the presence of calcium ions. The sulfate content and the anticoagulant activity of the dermatan sulfate fraction isolated in the precipitate were three and four times greater respectively than the starting material. Our in vivo studies showed that this fraction has threefold higher thrombolytic activity than the DS. All these results suggest that this fraction could be used as a therapeutic agent for thrombi dissolution. © 2004 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein
Autor:Calabrese, G.C.; Alberto, M.F.; Tubio, R.; Marani, M.M.; Fernández De Recondo, M.E.; Lazzari, M.; Recondo, E.F.
Filiación:Depto. de Ciencias Biológicas, Fac. de Farmacia Y Bioquímica, Universidad de Buenos Aires, Junín 954, Cd. Autonoma de Buenos Aires, 1113, Argentina
Depto. de Hemostasia Y Trombosis, Academia Nacional de Medicina, Cd. Autónoma de Buenos Aires, Argentina
Depto. de Quím. Biol., Fac. de Ciencias Exactas Y Naturales, Ciudad Universitaria, Pabellón II, Cd. Autonoma de Buenos Aires, 1428, Argentina
Juncal 2186, 60 P, Buenos Aires (1125), Argentina
Palabras clave:antithrombin; AT; C1; Complement system; Con A; Concanavalin A; Dermatan sulfate; DS; free Dermatan sulfate; free DS; GAG; glycosaminoglycan; Heparin cofactor II; HS; Molecular interactions; the first protein complex of the complement system; calcium ion; dermatan sulfate; fibrinolytic agent; heparin cofactor II; plasma protein; protein complex c1; unclassified drug; animal experiment; animal model; animal tissue; anticoagulation; article; complement system; dissolution; drug activity; drug structure; human; ionic strength; male; nonhuman; normal human; precipitation; priority journal; rat; thrombosis; Acetylgalactosamine; Animals; Anticoagulants; Calcium; Complement C1; Dermatan Sulfate; Fibrinolytic Agents; Hexosamines; Iduronic Acid; Male; Osmolar Concentration; Precipitation; Rats; Rats, Wistar; Structure-Activity Relationship; Sulfates
Año:2004
Volumen:113
Número:3-4
Página de inicio:243
Página de fin:250
DOI: http://dx.doi.org/10.1016/j.thromres.2004.01.014
Título revista:Thrombosis Research
Título revista abreviado:Thromb. Res.
ISSN:00493848
CODEN:THBRA
CAS:calcium ion, 14127-61-8; dermatan sulfate, 24967-94-0; heparin cofactor II, 81604-65-1; Acetylgalactosamine, 31022-50-1; Anticoagulants; Calcium, 7440-70-2; Complement C1; Dermatan Sulfate, 24967-94-0; Fibrinolytic Agents; Hexosamines; Iduronic Acid, 3402-98-0; Sulfates
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00493848_v113_n3-4_p243_Calabrese

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Citas:

---------- APA ----------
Calabrese, G.C., Alberto, M.F., Tubio, R., Marani, M.M., Fernández De Recondo, M.E., Lazzari, M. & Recondo, E.F. (2004) . A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein. Thrombosis Research, 113(3-4), 243-250.
http://dx.doi.org/10.1016/j.thromres.2004.01.014
---------- CHICAGO ----------
Calabrese, G.C., Alberto, M.F., Tubio, R., Marani, M.M., Fernández De Recondo, M.E., Lazzari, M., et al. "A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein" . Thrombosis Research 113, no. 3-4 (2004) : 243-250.
http://dx.doi.org/10.1016/j.thromres.2004.01.014
---------- MLA ----------
Calabrese, G.C., Alberto, M.F., Tubio, R., Marani, M.M., Fernández De Recondo, M.E., Lazzari, M., et al. "A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein" . Thrombosis Research, vol. 113, no. 3-4, 2004, pp. 243-250.
http://dx.doi.org/10.1016/j.thromres.2004.01.014
---------- VANCOUVER ----------
Calabrese, G.C., Alberto, M.F., Tubio, R., Marani, M.M., Fernández De Recondo, M.E., Lazzari, M., et al. A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein. Thromb. Res. 2004;113(3-4):243-250.
http://dx.doi.org/10.1016/j.thromres.2004.01.014