Artículo
Abstract:
Antithrombin (AT) high affinity of unfractionated heparin (UFH) resides in a specific pentasaccharide sequence. Heparin also regulates complement activity on the classical and the alternative pathways. Most experimental pieces of evidence accumulated show that these important activities reside in different segments of the heparin molecule. We demonstrated in previous papers that a low ionic strength and the presence of calcium ions are essential to detect specific interactions between glycosaminoglycans and proteins. Then these very strict conditions were used, and we demonstrated that the first protein complex of the human complement cascade recognizes in the UFH a fraction with very high anticoagulant activity. After isolation from the precipitate of the interaction, this fraction of heparin also contained the pentasaccharide sequence responsible for the great affinity with AT: in fact, it was strongly bound to a resin of AT agarose, and to detach it, an ionic strength of 0.6 M sodium chloride was required. In this way, the heparin regions responsible for the anticoagulant activity and also for the effects over the complement system were identified on the same short segment of the heparin molecule, which includes the active fraction of the glycosaminoglycan. The differences with early results could be explained by our experimental conditions of low ionic strength and the presence of calcium ions used for the interaction of the protein and the glycosaminoglycan. © 2002 Published by Elsevier Science Ltd.
Registro:
| Documento: | Artículo |
| Título: | Antithrombin and first complement protein recognize the same active heparin fraction |
| Autor: | Calabrese, G.C.; Recondo, E.F.; Fernandez De Recondo, M.E. |
| Filiación: | Departamento de Ciencias Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 954/956, Buenos Aires 1110, Argentina Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellón II, 4 Piso, Nuez, Buenos Aires, 1428, Argentina |
| Palabras clave: | Antithrombin; C1; Divalent cations; Heparin; Interactions; agarose; antithrombin; calcium ion; complement; glycosaminoglycan; heparin; pentasaccharide; resin; sodium chloride; anticoagulant agent; antithrombin III; calcium; complement component C1; heparin; anticoagulation; article; binding affinity; complement system; complex formation; ionic strength; priority journal; protein binding; affinity chromatography; binding site; human; isolation and purification; metabolism; osmolarity; precipitation; Anticoagulants; Antithrombin III; Binding Sites; Calcium; Chromatography, Affinity; Complement 1; Heparin; Human; Osmolar Concentration; Precipitation; Protein Binding; Support, Non-U.S. Gov't; Anticoagulants; Binding Sites; Calcium; Chromatography, Affinity; Complement C1; Humans; Osmolar Concentration; Precipitation; Protein Binding |
| Año: | 2002 |
| Volumen: | 105 |
| Número: | 6 |
| Página de inicio: | 537 |
| Página de fin: | 541 |
| DOI: | http://dx.doi.org/10.1016/S0049-3848(02)00062-2 |
| Título revista: | Thrombosis Research |
| Título revista abreviado: | Thromb. Res. |
| ISSN: | 00493848 |
| CODEN: | THBRA |
| CAS: | Anticoagulants; Antithrombin III, 9000-94-6; Calcium, 7440-70-2; Complement 1; Heparin, 9005-49-6; Complement C1 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00493848_v105_n6_p537_Calabrese |
Referencias:
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Citas:
---------- APA ----------
Calabrese, G.C., Recondo, E.F. & Fernandez De Recondo, M.E. (2002) . Antithrombin and first complement protein recognize the same active heparin fraction. Thrombosis Research, 105(6), 537-541.http://dx.doi.org/10.1016/S0049-3848(02)00062-2
---------- CHICAGO ----------
Calabrese, G.C., Recondo, E.F., Fernandez De Recondo, M.E. "Antithrombin and first complement protein recognize the same active heparin fraction" . Thrombosis Research 105, no. 6 (2002) : 537-541.http://dx.doi.org/10.1016/S0049-3848(02)00062-2
---------- MLA ----------
Calabrese, G.C., Recondo, E.F., Fernandez De Recondo, M.E. "Antithrombin and first complement protein recognize the same active heparin fraction" . Thrombosis Research, vol. 105, no. 6, 2002, pp. 537-541.http://dx.doi.org/10.1016/S0049-3848(02)00062-2
---------- VANCOUVER ----------
Calabrese, G.C., Recondo, E.F., Fernandez De Recondo, M.E. Antithrombin and first complement protein recognize the same active heparin fraction. Thromb. Res. 2002;105(6):537-541.http://dx.doi.org/10.1016/S0049-3848(02)00062-2
