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Abstract:

Environmental contaminants produce multiple adverse consequences at individual, population and ecosystem levels. High volumes of agrochemicals applied to great variety of crops, together with agricultural expansion, generate great concerns due to the impact for the environment and large risk implicated for wildlife. The lack of data on these threats is striking. The tegu lizard (Salvator merianae) is one of the species that live in environments under contaminant effects. Several characteristics allow proposing this species as a potential sentinel organism for the monitoring of pesticides in their habitat. The present study is the first report about genotoxicity in tegu lizard neonates after embryonic exposure to Roundup® (glyphosate 66.2%). The micronucleus test (MN), nuclear abnormalities (NAs) assay and comet assay (CA) were used as biomarkers of genotoxic effects induced in erythrocytes by topical exposure of the eggs to the glyphosate commercial formulation Roundup® (RU), in laboratory controlled conditions. A total of 96 eggs were distributed in six groups exposed to RU (50, 100, 200, 400, 800, 1600 μg/egg), one positive control (PC; 200 μg cyclophosphamide/egg) and one negative control (NC; distilled water). No teratogenic effects were observed in any of the exposed or control neonates. A significant increase in DNA damage was observed in all concentrations higher than 100 μg/egg with respect to NC (p < 0.05). However, no statistical differences were found in the frequencies of MN and NAs in any group exposed to RU compared to the NC. No statistically significant differences were found in the size of the lizards at birth or after six months post-exposure (p > 0.05). Our results provide new information about the undesirable effects of the glyphosate-based herbicide formulations RU on this lizard species that inhabits areas permanently exposed to several pesticide formulations. We consider of utmost necessity a strict regulation of the agrochemical application conditions in those environments near to places where wild populations of terrestrial and aquatic species live, in order to minimize the adverse effects on ecosystems. © 2015 Elsevier B.V.

Registro:

Documento: Artículo
Título:Genotoxicity induced by Roundup® (Glyphosate) in tegu lizard (Salvator merianae) embryos
Autor:Schaumburg, L.G.; Siroski, P.A.; Poletta, G.L.; Mudry, M.D.
Filiación:Grupo de Investigación en Biología Evolutiva (GIBE), IEGEBA (CONICET-UBA), FCEyN, Pabellon II - 4to Piso. Labs 43-46. Cdad. Universitaria, Buenos Aires, CP: 1428EGA, Argentina
Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC, UNL/MASPyMA), Aristóbulo del Valle 8700, Santa Fe, CP: 3000, Argentina
Instituto de Ciencias Veterinarias del Litoral (ICiVet-CONICET), R.P. Kreder 2805, Esperanza, Santa Fe, CP: S3080HOF, Argentina
Cátedra de Toxicología, Farmacología y Bioquímica Legal (FBCB, UNL), Ciudad Universitaria Paraje El Pozo, Santa Fe, CP: 3000, Argentina
Palabras clave:Eggs; Genotoxic effects; Glyphosate-based formulation; Tegu lizard; glycine; glyphosate; herbicide; analogs and derivatives; animal; comet assay; dose response; drug effects; embryo development; growth, development and aging; lizard; micronucleus test; nonmammalian embryo; Animals; Comet Assay; Dose-Response Relationship, Drug; Embryo, Nonmammalian; Embryonic Development; Glycine; Herbicides; Lizards; Micronucleus Tests
Año:2016
Volumen:130
Página de inicio:71
Página de fin:78
DOI: http://dx.doi.org/10.1016/j.pestbp.2015.11.009
Título revista:Pesticide Biochemistry and Physiology
Título revista abreviado:Pestic. Biochem. Physiol.
ISSN:00483575
CODEN:PCBPB
CAS:glycine, 56-40-6, 6000-43-7, 6000-44-8; glyphosate, 1071-83-6; Glycine; glyphosate; Herbicides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00483575_v130_n_p71_Schaumburg

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Citas:

---------- APA ----------
Schaumburg, L.G., Siroski, P.A., Poletta, G.L. & Mudry, M.D. (2016) . Genotoxicity induced by Roundup® (Glyphosate) in tegu lizard (Salvator merianae) embryos. Pesticide Biochemistry and Physiology, 130, 71-78.
http://dx.doi.org/10.1016/j.pestbp.2015.11.009
---------- CHICAGO ----------
Schaumburg, L.G., Siroski, P.A., Poletta, G.L., Mudry, M.D. "Genotoxicity induced by Roundup® (Glyphosate) in tegu lizard (Salvator merianae) embryos" . Pesticide Biochemistry and Physiology 130 (2016) : 71-78.
http://dx.doi.org/10.1016/j.pestbp.2015.11.009
---------- MLA ----------
Schaumburg, L.G., Siroski, P.A., Poletta, G.L., Mudry, M.D. "Genotoxicity induced by Roundup® (Glyphosate) in tegu lizard (Salvator merianae) embryos" . Pesticide Biochemistry and Physiology, vol. 130, 2016, pp. 71-78.
http://dx.doi.org/10.1016/j.pestbp.2015.11.009
---------- VANCOUVER ----------
Schaumburg, L.G., Siroski, P.A., Poletta, G.L., Mudry, M.D. Genotoxicity induced by Roundup® (Glyphosate) in tegu lizard (Salvator merianae) embryos. Pestic. Biochem. Physiol. 2016;130:71-78.
http://dx.doi.org/10.1016/j.pestbp.2015.11.009