Artículo

Lascano, C.I.; Ferrari, A.; Gauna, L.E.; Cocca, C.; Cochón, A.C.; Verrengia, N.; Venturino, A. "Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesis" (2011) Pesticide Biochemistry and Physiology. 101(3):240-247
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Abstract:

The objective of the present study was to evaluate the concentration- and time-dependent effects of the organophosphorus insecticides malathion and azinphos-methyl on polyamine metabolism, and relate them to normal and altered embryonic development of the common toad Rhinella arenarum. Control embryos showed that the higher polyamines spermidine and spermine acquired importance with respect to the diamine putrescine as embryonic development progressed. The activity of ornithine decarboxylase significantly decreased in complete operculum embryos. Continuous exposure to malathion caused a decrease in polyamine levels during embryonic development. However, there was an increase in putrescine levels in complete operculum embryos exposed to a sublethal concentration of the insecticide. Embryos exposed to malathion displayed a decrease in fresh weight and size, along with an increase in the number of malformed individuals. R. arenarum embryos exposed to a lethal concentration of azinphos-methyl showed an increase in putrescine levels and a decrease in spermidine and spermine levels, accompanied by an increase in ornithine decarboxylase activity. In conclusion, as the embryonic development of the toad R. arenarum progresses, polyamine metabolism shifts to higher polyamine levels with a more preponderant contribution of spermidine and spermine with respect to putrescine and involves a dramatic change in ornithine decarboxylase activity, one of the key regulatory enzymes of the pathway. Organophosphorus insecticides are capable of altering polyamine metabolism, slowing embryo development in parallel with a reduction in spermidine and spermine levels. An increase in the oxidative degradation of polyamines might be involved in the toxic action of organophosphorus insecticides and might also be related to other effects such as teratogenesis. © 2011 Elsevier Inc.

Registro:

Documento: Artículo
Título:Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesis
Autor:Lascano, C.I.; Ferrari, A.; Gauna, L.E.; Cocca, C.; Cochón, A.C.; Verrengia, N.; Venturino, A.
Filiación:LIBIQUIMA, Facultad de Ingeniería, IDEPA, Universidad Nacional del Comahue - CONICET, Buenos Aires 1400, 8300 Neuquén, Argentina
Facultad de Ciencias Agrarias, Universidad Nacional del Comahue, Ruta 151Km 12.5, 8303 Cinco Saltos, Río Negro, Argentina
Facultad de Ciencias Médicas, Universidad Nacional del Comahue, Toschi y Arrayanes, 8324 Cipolletti, Río Negro, Argentina
Laboratorio de Radioisótopos, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 954, Ciudad Autónoma de Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab. II. 4 piso, Nuñez, 1428 Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:Malformations; Organophosphorus pesticides; Ornithine decarboxylase; Putrescine; Spermidine; Spermine; Amphibia; Anura
Año:2011
Volumen:101
Número:3
Página de inicio:240
Página de fin:247
DOI: http://dx.doi.org/10.1016/j.pestbp.2011.10.001
Título revista:Pesticide Biochemistry and Physiology
Título revista abreviado:Pestic. Biochem. Physiol.
ISSN:00483575
CODEN:PCBPB
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00483575_v101_n3_p240_Lascano

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Citas:

---------- APA ----------
Lascano, C.I., Ferrari, A., Gauna, L.E., Cocca, C., Cochón, A.C., Verrengia, N. & Venturino, A. (2011) . Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesis. Pesticide Biochemistry and Physiology, 101(3), 240-247.
http://dx.doi.org/10.1016/j.pestbp.2011.10.001
---------- CHICAGO ----------
Lascano, C.I., Ferrari, A., Gauna, L.E., Cocca, C., Cochón, A.C., Verrengia, N., et al. "Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesis" . Pesticide Biochemistry and Physiology 101, no. 3 (2011) : 240-247.
http://dx.doi.org/10.1016/j.pestbp.2011.10.001
---------- MLA ----------
Lascano, C.I., Ferrari, A., Gauna, L.E., Cocca, C., Cochón, A.C., Verrengia, N., et al. "Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesis" . Pesticide Biochemistry and Physiology, vol. 101, no. 3, 2011, pp. 240-247.
http://dx.doi.org/10.1016/j.pestbp.2011.10.001
---------- VANCOUVER ----------
Lascano, C.I., Ferrari, A., Gauna, L.E., Cocca, C., Cochón, A.C., Verrengia, N., et al. Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesis. Pestic. Biochem. Physiol. 2011;101(3):240-247.
http://dx.doi.org/10.1016/j.pestbp.2011.10.001