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Abstract:

Glyphosate-based products are the leading post-emergent agricultural herbicides in the world, particularly in association with glyphosate tolerant crops. However, studies on the effects of glyphosate-based formulations on terrestrial receptors are scarce. This study was conducted to evaluate the comparative toxicity of two glyphosate-based products: Roundup FG (monoammonium salt, 72% acid equivalent, glyphosate-A) and Mon 8750 (monoammonium salt, 85.4% acid equivalent, glyphosate-B), towards the earthworm Eisenia andrei. Median lethal concentration (LC50) showed that glyphosate-A was 4.5-fold more toxic than glyphosate-B. Sublethal concentrations caused a concentration-dependent weight loss, consistent with the reported effect of glyphosate as uncoupler of oxidative phosphorylation. Glyphosate-A showed deleterious effects on DNA and lysosomal damage at concentrations close to the applied environmental concentrations (14.4μg ae cm-2). With glyphosate-B toxic effects were observed at higher doses, close to its LC50, suggesting that the higher toxicity of formulate A could be attributed to the effects of some of the so-called "inert ingredients", either due to a direct intrinsic toxicity, or to an enhancement in the bioavailability and/or bioaccumulation of the active ingredient. Our results highlight the importance of ecotoxicological assessment not only of the active ingredients, but also of the different formulations usually employed in agricultural practices. © 2012 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Comparative toxicity of two glyphosate-based formulations to Eisenia andrei under laboratory conditions
Autor:Piola, L.; Fuchs, J.; Oneto, M.L.; Basack, S.; Kesten, E.; Casabé, N.
Filiación:Toxicology and Legal Chemistry, Department of Biological Chemistry, Faculty of Exact and Natural Sciences, University of Buenos Aires, Pabellón 2, 4o piso, Ciudad Universitaria, 1428 Buenos Aires, Argentina
IQUIBICEN-UBA/CONICET, Department of Biological Chemistry, FCEN, UBA, Pabellón 2, 4o piso, Ciudad Universitaria, Argentina
Palabras clave:Comet assay; Eisenia andrei; Glyphosate formulations; Neutral red retention time; Sublethal biomarkers; Comet assays; Ecotoxicological assessment; Eisenia andrei; Environmental concentration; Glyphosates; Median Lethal concentration; Oxidative phosphorylation; Retention time; Agriculture; Biochemistry; Toxicity; Herbicides; ammonium derivative; glyphosate; agricultural practice; bioavailability; biomarker; comparative study; concentration (composition); dose-response relationship; earthworm; ecotoxicology; glyphosate; laboratory method; pollution effect; toxicity test; weight; article; bioaccumulation; bioavailability; cell damage; comet assay; comparative study; controlled study; DNA damage; earthworm; Eisenia andrei; growth; LC 50; lysosome; nonhuman; oxidative phosphorylation; toxicity; weight reduction; Animals; Glycine; Herbicides; Laboratories; Lethal Dose 50; Oligochaeta; Risk Assessment; Soil Pollutants; Eisenia andrei
Año:2013
Volumen:91
Número:4
Página de inicio:545
Página de fin:551
DOI: http://dx.doi.org/10.1016/j.chemosphere.2012.12.036
Título revista:Chemosphere
Título revista abreviado:Chemosphere
ISSN:00456535
CODEN:CMSHA
CAS:glyphosate, 1071-83-6; Glycine, 56-40-6; Herbicides; Soil Pollutants; glyphosate, 1071-83-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00456535_v91_n4_p545_Piola

Referencias:

  • Bonnet, J.L., Bonnemoy, F., Dusser, M., Bohatier, J., Assessment of the potential toxicity of herbicides and their degradation products to non-target cells using two microorganisms, the bacteria Vibrio fischeri and the ciliate Tetrahymena pyriformis (2007) Environ. Toxicol., 22, pp. 78-91
  • Booth, L., O'Halloran, K., A comparison of biomarker responses in the earthworm Aporrectodea caliginosa to the organophosphorus insecticides diazinon and chlorpyrifos (2001) Environ. Toxicol. Chem., 20, pp. 2494-2502
  • Casabé, N., Piola, L., Fuchs, J., Oneto, M.L., Pamparato, L., Basack, S., Giménez, R., Kesten, E., Ecotoxicological assessment of the effects of glyphosate and chlorpyrifos in an argentine soya field (2007) J. Soils Sediments, 7, pp. 232-239
  • (2011), http://www.casafe.org/estadisticas.php, Casafe. Cámara de sanidad agropecuaria y fertilizantes; Correia, F.V., Moreira, J.C., Effects of glyphosate and 2,4-D on earthworms (Eisenia foetida) in laboratory tests (2010) Bull. Environ. Contam. Toxicol., 85, pp. 264-268
  • Domingues, I.S., Agra, A.R., Monaghan, K., Soares, A.M.V.M., Nogueira, A.J.A., Cholinesterase and glutathione-S-transferase activities in freshwater invertebrates as biomarkers to assess pesticide contamination (2010) Environ. Toxicol. Chem., 29, pp. 5-18
  • (2009), Environment Agency,. Standard Operating Procedures for Bioassays in Ecological Risk Assessment. Science report - SC070009. Environment Agency, Bristol; Fuchs, J., Piola, L., Prieto González, E., Oneto, M.L., Basack, S., Kesten, E., Casabé, N., Coelomocyte biomarkers in the earthworm Eisenia fetida exposed to 2,4,6-trinitrotoluene (TNT) (2011) Environ. Monit. Assess., 175, pp. 127-137
  • Hwang, H.M., Wade, T.L., Sericano, J.L., Destabilized lysosomes and elimination of polycyclic aromatic hydrocarbons and polychlorinated biphenyls in eastern oysters (Crassostrea virginica) (2004) Environ. Toxicol. Chem., 23, pp. 1991-1995
  • Klobučar, G., Stambuk, A., Srut, M., Husnjak, I., Merka, M., Traven, L., Cvetkovic, Z., Aporrectodea caliginosa, a suitable earthworm species for field based genotoxicity assessment? (2011) Environ. Pollut., 159, pp. 841-849
  • Lajmanovich, R., Attademo, A., Peltzer, P., Junges, C., Cabagna, M., Toxicity of four herbicide formulations with glyphosate on Rhinella arenarum (Anura: Bufonidae) tadpoles: B-esterases and glutathione-S-transferase inhibitors (2011) Arch. Environ. Contam. Toxicol., 60, pp. 681-689
  • Lamb, D.C., Kelly, D.E., Hanley, S.Z., Mehmood, Z., Kelly, S.L., Glyphosate is an inhibitor of plant cytochrome P450: functional expression of Thlaspi arvensae cytochrome P45071B1/reductase fusion protein in Escherichia coli (1998) Biochem. Biophys. Res. Commun., 244, pp. 110-114
  • Monteiro, D., Cavalcante, D.G., Viléla, M.V., Sofia, S.H., Martinez, C.B.R., In vivo and in vitro exposures for the evaluation of the genotoxic effects of lead on the Neotropical freshwater fish Prochilodus lineatus (2011) Aquat. Toxicol., 104, pp. 291-298
  • Muangphra, P., Kwankua, W., Gooneratne, R., Genotoxic effects of glyphosate or paraquat on earthworm coelomocytes. Environ. Toxicol. (2012), 13. doi: 10.1002/tox.21787. (Article first published online: 30 May 2012); (1984) Guideline for Testing of Chemicals No. 207. Earthworm Acute Toxicity Tests, , OECD. Organization for Economic Cooperation and Development, Paris, France
  • Pereira, J.L., Antunes, S.C., Castro, B.B., Marques, C.R., Gonçalves, A.M.M., Gonçalves, F., Pereira, R., Toxicity evaluation of three pesticides on non-target aquatic and soil organisms: commercial formulation versus active ingredient (2009) Ecotoxicology, 18, pp. 455-463
  • Reinecke, S., Reinecke, A., The comet assay as biomarker of heavy metal genotoxicity in earthworms (2004) Arch. Environ. Contam. Toxicol., 46, pp. 208-215
  • Roberts, B.L., Dorough, H.W., Relative toxicities of chemicals to the earthworm Eisenia foetida (1984) Environ. Toxicol. Chem., 3, pp. 67-68
  • Robidoux, P.Y., Svendsen, C., Sarrazin, M., Hawari, J., Thiboutot, S., Ampleman, G., Weeks, J.M., Sunahara, G.I., Evaluation of tissue and cellular biomarkers to assess 2,4,6-trinitrotoluene (TNT) exposure in earthworms: effects-based assessment in laboratory studies using Eisenia andrei (2002) Biomarkers, 7, pp. 306-321
  • Römbke, J., Heimbach, F., Hoy, S., Kula, C., Scott-Fordsmand, J., Sousa, J.P., Stephenson, G., Weeks, J., (2003) Effects of Plant Protection Products on Functional Endpoints in Soil (EPFES), p. 109. , SETAC Press, Pensacola, USA
  • Sánchez-Hernández, J.C., Earthworm biomarkers in ecological risk assessment (2006) Rev. Environ. Contam. Toxicol., 188, pp. 85-126
  • Santos, M.J., Morgado, R., Ferreira, N.G., Soares, A.M., Loureiro, S., Evaluation of the joint effect of glyphosate and dimethoate using a small-scale terrestrial ecosystem (2011) Ecotoxicol. Environ. Saf., 74, pp. 1994-2001
  • Scott-Fordsmand, J.J., Weeks, J.M., Biomarkers in earthworms (2000) Rev. Environ. Contam. Toxicol., 165, pp. 117-159
  • , p. 336. , http://www.fs.fed.us/foresthealth/pesticide/pdfs/Glyphosate_SERA_TR-052-22-03b.pdf, USDA/FS, 2011. United States Department of Agriculture/Forest service. Glyphosate - human health and ecological risk assessment (SERA TR-052-22-03b). Final report, March 2011; Wang, Y., Wu, S., Chen, L., Wu, C., Yu, R., Wang, Q., Zhao, X., Toxicity assessment of 45 pesticides to the epigeic earthworm Eisenia fetida (2012) Chemosphere, 88, pp. 484-491
  • Weeks, J., Svendsen, C., Neutral red retention by lysosomes from earthworm (Lumbricus rubellus) coelomocytes: a simple biomarker of exposure to soil copper (1996) Environ. Toxicol. Chem., 15, pp. 1801-1805
  • (1994) Environmental Health Criteria 159 (Glyphosate), , WHO. World Health Organization, Geneva
  • Xiao, N.W., Song, Y., Ge, F., Liu, X.H., Ou-Yang, Z.Y., Biomarker responses of the earthworm Eisenia fetida to acetochlor exposure in OECD soil (2006) Chemosphere, 65, pp. 907-912
  • Yasmin, S., D'Souza, D., Effect of pesticides on the reproductive output of Eisenia fetida (2007) Bull. Environ. Contam. Toxicol., 79, pp. 529-532

Citas:

---------- APA ----------
Piola, L., Fuchs, J., Oneto, M.L., Basack, S., Kesten, E. & Casabé, N. (2013) . Comparative toxicity of two glyphosate-based formulations to Eisenia andrei under laboratory conditions. Chemosphere, 91(4), 545-551.
http://dx.doi.org/10.1016/j.chemosphere.2012.12.036
---------- CHICAGO ----------
Piola, L., Fuchs, J., Oneto, M.L., Basack, S., Kesten, E., Casabé, N. "Comparative toxicity of two glyphosate-based formulations to Eisenia andrei under laboratory conditions" . Chemosphere 91, no. 4 (2013) : 545-551.
http://dx.doi.org/10.1016/j.chemosphere.2012.12.036
---------- MLA ----------
Piola, L., Fuchs, J., Oneto, M.L., Basack, S., Kesten, E., Casabé, N. "Comparative toxicity of two glyphosate-based formulations to Eisenia andrei under laboratory conditions" . Chemosphere, vol. 91, no. 4, 2013, pp. 545-551.
http://dx.doi.org/10.1016/j.chemosphere.2012.12.036
---------- VANCOUVER ----------
Piola, L., Fuchs, J., Oneto, M.L., Basack, S., Kesten, E., Casabé, N. Comparative toxicity of two glyphosate-based formulations to Eisenia andrei under laboratory conditions. Chemosphere. 2013;91(4):545-551.
http://dx.doi.org/10.1016/j.chemosphere.2012.12.036