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Abstract:

Azinphos-methyl is an organophosphate insecticide used for pest control on a number of food crops in many parts of the world. The oligochaete Lumbriculus variegatus and pigmented and non-pigmented specimens of the gastropod Biomphalaria glabrata are freshwater invertebrates that have been recommended for contamination studies. Recently, it has been shown that L. variegatus worms exhibit a higher cholinesterase (ChE) activity and a greater sensitivity to in vivo ChE inhibition by azinphos-methyl than pigmented B. glabrata snails. The aims of the present study were (1) to investigate if, in addition to its anticholinesterase action, azinphos-methyl has also pro-oxidant activity in L. variegatus and B. glabrata, and (2) to examine if species that are highly susceptible to the neurotoxic effects of organophosphates also suffer a greater degree of oxidative stress. Therefore, total glutathione (t-GSH) levels and activities of cholinesterase (ChE), superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and glucose 6-phosphate dehydrogenase (G6PDH) were measured in the whole body soft tissue of organisms exposed for 48 and 96 h to a level of azinphos-methyl that produces 50% of inhibition on ChE. Results showed different patterns of antioxidant responses between the gastropods and the oligochaetes, and even between the two phenotypes of gastropods: (1) in exposed L. variegatus t-GSH levels increased and CAT and SOD activities decreased with respect to control organisms, (2) in pigmented gastropods, SOD decreased while CAT transiently diminished, and (3) in non-pigmented gastropods, SOD activity showed a biphasic response. GST and G6PDH were not altered by azinphos-methyl exposure. Of note, t-GSH levels were 4-fold times higher in L. variegatus than in both phenotypes of B. glabrata. This may suggest that GSH could play a more important role in antioxidant defense in L. variegatus than in B. glabrata. © 2008 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Effects of azinphos-methyl exposure on enzymatic and non-enzymatic antioxidant defenses in Biomphalaria glabrata and Lumbriculus variegatus
Autor:Kristoff, G.; Verrengia Guerrero, N.R.; Cochón, A.C.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Nunez, Pabellon II, 4to piso, 1428 Buenos Aires, Argentina
Palabras clave:Catalase; Cholinesterase; Freshwater invertebrate; Glutathione; Organophosphate; Superoxide dismutase; Amperometric sensors; Canning; Enzymes; Food preservation; Glucose; Insect control; Microfluidics; Occupational diseases; Oxygen; Pest control; Photoacoustic effect; Plants (botany); System theory; (abiotic and biotic) stress; Antioxidant defenses; Antioxidant responses; Azinphos methyl (AZP); Biphasic response; Catalase (CAT); Cholinesterase (ChE); Elsevier (CO); food crops; Freshwater invertebrates; Glucose-6-phosphate dehydrogenase (G6PDH); Glutathione (GSH); Glutathione-S-transferase (GST); In-vivo; Neurotoxic effects; Non-enzymatic; SOD activity; soft tissues; Superoxide dismutase (SOD1); Whole body (WB); Agricultural engineering; azinphos methyl; catalase; cholinesterase; glucose 6 phosphate dehydrogenase; glutathione; glutathione transferase; organophosphate; superoxide dismutase; annelid; antioxidant; defense mechanism; enzyme activity; gastropod; insecticide; organophosphate; pollution effect; pollution exposure; animal tissue; annelid worm; antioxidant activity; article; Biomphalaria glabrata; controlled study; enzyme activity; gastropod; Lumbriculus variegatus; neurotoxicity; nonhuman; oxidative stress; phenotype; soft tissue; Animals; Antioxidants; Azinphosmethyl; Biomphalaria; Catalase; Cholinesterases; Enzymes; Glutathione; Glyceraldehyde-3-Phosphate Dehydrogenases; Insecticides; Oligochaeta; Phenotype; Proteins; Superoxide Dismutase; Biomphalaria glabrata; Gastropoda; Invertebrata; Lumbriculus variegatus; Oligochaeta (Metazoa)
Año:2008
Volumen:72
Número:9
Página de inicio:1333
Página de fin:1339
DOI: http://dx.doi.org/10.1016/j.chemosphere.2008.04.026
Título revista:Chemosphere
Título revista abreviado:Chemosphere
ISSN:00456535
CODEN:CMSHA
CAS:azinphos methyl, 86-50-0; catalase, 9001-05-2; cholinesterase, 9001-08-5; glucose 6 phosphate dehydrogenase, 37259-83-9, 9001-40-5; glutathione transferase, 50812-37-8; glutathione, 70-18-8; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; Antioxidants; Azinphosmethyl, 86-50-0; Catalase, EC 1.11.1.6; Cholinesterases, EC 3.1.1.8; Enzymes; Glutathione, 70-18-8; Glyceraldehyde-3-Phosphate Dehydrogenases, EC 1.2.1.-; Insecticides; Proteins; Superoxide Dismutase, EC 1.15.1.1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00456535_v72_n9_p1333_Kristoff

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Citas:

---------- APA ----------
Kristoff, G., Verrengia Guerrero, N.R. & Cochón, A.C. (2008) . Effects of azinphos-methyl exposure on enzymatic and non-enzymatic antioxidant defenses in Biomphalaria glabrata and Lumbriculus variegatus. Chemosphere, 72(9), 1333-1339.
http://dx.doi.org/10.1016/j.chemosphere.2008.04.026
---------- CHICAGO ----------
Kristoff, G., Verrengia Guerrero, N.R., Cochón, A.C. "Effects of azinphos-methyl exposure on enzymatic and non-enzymatic antioxidant defenses in Biomphalaria glabrata and Lumbriculus variegatus" . Chemosphere 72, no. 9 (2008) : 1333-1339.
http://dx.doi.org/10.1016/j.chemosphere.2008.04.026
---------- MLA ----------
Kristoff, G., Verrengia Guerrero, N.R., Cochón, A.C. "Effects of azinphos-methyl exposure on enzymatic and non-enzymatic antioxidant defenses in Biomphalaria glabrata and Lumbriculus variegatus" . Chemosphere, vol. 72, no. 9, 2008, pp. 1333-1339.
http://dx.doi.org/10.1016/j.chemosphere.2008.04.026
---------- VANCOUVER ----------
Kristoff, G., Verrengia Guerrero, N.R., Cochón, A.C. Effects of azinphos-methyl exposure on enzymatic and non-enzymatic antioxidant defenses in Biomphalaria glabrata and Lumbriculus variegatus. Chemosphere. 2008;72(9):1333-1339.
http://dx.doi.org/10.1016/j.chemosphere.2008.04.026