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Abstract:

Cherax quadricarinatus displays biological attributes that make it suitable for commercial aquaculture and, as a freshwater species, it has high starvation resistance. Previous studies demonstrated that after 40days of starvation only the 25% (PNR25) and after 50days the 50% (PNR50) of one-gram juveniles died. The objective of this study is to characterize the pattern of use of energetic reserves through the analysis of digestive enzyme activities and the occurrence of lipids, proteins and glycogen in the hepatopancreas and pleon muscle, nearby PNR25, PNR50 and after a feeding period. One-gram juveniles were randomly assigned to one of two feeding protocols: continuous feeding throughout 90-days (control) and starvation until day 50 and then feeding for the following 40days (treatment). Juveniles from each feeding protocol were weighed, measured and sacrificed at day 40, 50 or 90. Total lipids, glycogen and proteins were determined on hepatopancreas and pleon; and lipase, amylase and protease activities were also estimated in the hepatopancreas. Growth stopped during starvation and resumed when food was supplied. Close to the point-of-no-return 50 pleon muscle began to degrade diminishing protein content and the lipid content decreases significantly in the hepatopancreas. However, after the feeding period both reserves were completely replenished. Although glycogen levels were not affected during starvation, a pronounced accumulation of this nutrient in the pleon was triggered when food was available. The lipase activity decreased during starvation suggesting that the lipase whose activity was measured may not be synthesized when food is not available. Although starvation had no significant effect on the protease and amylase activities, they tended to decrease around the point-of-no-return and to increase after the feeding period. In this context, the present research provides new and relevant biological information on physiological and biochemical responses of crustaceans nearby PNR25 and PNR50. © 2013 Elsevier B.V.

Registro:

Documento: Artículo
Título:Energetic reserves and digestive enzyme activities in juveniles of the red claw crayfish Cherax quadricarinatus nearby the point-of-no-return
Autor:Calvo, N.S.; Stumpf, L.; Sacristán, H.J.; López Greco, L.S.
Filiación:Biology of Reproduction and Growth in Crustaceans, Department of Biodiversity and Experimental Biology, FCEyN, University of Buenos Aires, Cdad. Univ., C1428EGA Buenos Aires, Argentina
IBBEA, CONICET-UBA, Argentina
Palabras clave:Digestive enzymes; Energetic reserves; Hepatopancreas; Lipid; Pleon; PNR; bioaccumulation; biochemistry; bioenergetics; commercial species; crayfish; crustacean culture; digestion; enzyme activity; freshwater environment; growth response; juvenile; lipid; physiological response; starvation; Astacoidea; Cherax quadricarinatus; Crustacea
Año:2013
Volumen:416-417
Página de inicio:85
Página de fin:91
DOI: http://dx.doi.org/10.1016/j.aquaculture.2013.08.017
Título revista:Aquaculture
Título revista abreviado:Aquaculture
ISSN:00448486
CODEN:AQCLA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00448486_v416-417_n_p85_Calvo

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Citas:

---------- APA ----------
Calvo, N.S., Stumpf, L., Sacristán, H.J. & López Greco, L.S. (2013) . Energetic reserves and digestive enzyme activities in juveniles of the red claw crayfish Cherax quadricarinatus nearby the point-of-no-return. Aquaculture, 416-417, 85-91.
http://dx.doi.org/10.1016/j.aquaculture.2013.08.017
---------- CHICAGO ----------
Calvo, N.S., Stumpf, L., Sacristán, H.J., López Greco, L.S. "Energetic reserves and digestive enzyme activities in juveniles of the red claw crayfish Cherax quadricarinatus nearby the point-of-no-return" . Aquaculture 416-417 (2013) : 85-91.
http://dx.doi.org/10.1016/j.aquaculture.2013.08.017
---------- MLA ----------
Calvo, N.S., Stumpf, L., Sacristán, H.J., López Greco, L.S. "Energetic reserves and digestive enzyme activities in juveniles of the red claw crayfish Cherax quadricarinatus nearby the point-of-no-return" . Aquaculture, vol. 416-417, 2013, pp. 85-91.
http://dx.doi.org/10.1016/j.aquaculture.2013.08.017
---------- VANCOUVER ----------
Calvo, N.S., Stumpf, L., Sacristán, H.J., López Greco, L.S. Energetic reserves and digestive enzyme activities in juveniles of the red claw crayfish Cherax quadricarinatus nearby the point-of-no-return. Aquaculture. 2013;416-417:85-91.
http://dx.doi.org/10.1016/j.aquaculture.2013.08.017