Artículo

Lozada, M.; Itria, R.F.; Figuerola, E.L.M.; Babay, P.A.; Gettar, R.T.; De Tullio, L.A.; Erijman, L. "Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge" (2004) Water Research. 38(8):2077-2086
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Abstract:

A molecular approach was used to evaluate the effect of nonylphenol ethoxylate surfactants on the bacterial diversity in lab-scale activated sludge reactors. Separate bench-scale units were fed synthetic wastewater with and without addition of branched nonylphenol ethoxylates (NPnEO). The performance of the reactors, in terms of carbonaceous removal was largely unaffected by the presence of NP10EO in the feeding solution. However, addition of NP10EO exerted a pronounced shift in bacterial community composition. In situ hybridization analyzing larger phylogenetic groups of bacteria with ribosomal RNA-targeted oligonucleotide probes revealed the dominance of clusters composed of Betaproteobacteria, accounting for up to one-third of 4′,6-diamidino-2- phenylindol-dihydrochloride (DAPI)-stained cells in NP10EO amended reactors and only 5% of DAPI-stained cells in the controls. These shifts in populations of larger phylogenetic groups were confirmed by dot-blot analysis of rRNA. Members of gamma subclass of Proteobacteria were present in low numbers in all activated sludge samples examined, suggesting that only bacteria affiliated with the beta subclass of Proteobacteria may have a specific role in NP10EO degradation. © 2004 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge
Autor:Lozada, M.; Itria, R.F.; Figuerola, E.L.M.; Babay, P.A.; Gettar, R.T.; De Tullio, L.A.; Erijman, L.
Filiación:Inst. Invest. Ing. Genet. Y Biol. M., Fac. de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Ctro. Invest. Y Desarrollo Ing. A., Inst. Nac. de Tecn. Industrial, Paseo Colón 850, 1063 Buenos Aires, Argentina
Unidad de Actividad Química, Comn. Nac. de Ener. Atómica, Av. Gral. Paz 1499, Pcia. de Buenos Aires, Argentina
Palabras clave:Activated sludge; Betaproteobacteria; Community structure; Fluorescence in situ hybridization; Nonionic surfactant; Nonylphenol ethoxylates; Bacteria; Carbonaceous adsorbents; Composition; Degradation; Probes; Removal; Solutions; Surface active agents; Wastewater; Bacterial community; Bench-scale units; Feeding solutions; Sludge reactors; Sewage sludge; 4',6 diamidino 2 phenylindole; nonylphenol; nonylphenol ethoxylate; ribosome RNA; surfactant; unclassified drug; activated sludge; bacterium; surfactant; wastewater treatment; article; bacterial flora; bacterial strain; bacterium identification; controlled study; dot hybridization; evaluation; Gram negative bacterium; in situ hybridization; microbial diversity; nonhuman; oligonucleotide probe; priority journal; reactor; separation technique; sludge; waste water management; Aerobiosis; Animals; Bacteria; Base Sequence; Betaproteobacteria; Biodegradation, Environmental; Ethylene Glycols; Fishes; Fluorescent Dyes; Gammaproteobacteria; In Situ Hybridization; Oligonucleotide Probes; RNA, Ribosomal, 16S; Sewage; Soil Microbiology; Surface-Active Agents; Time Factors; Bacteria (microorganisms); Betaproteobacteria; Proteobacteria
Año:2004
Volumen:38
Número:8
Página de inicio:2077
Página de fin:2086
DOI: http://dx.doi.org/10.1016/j.watres.2004.01.032
Título revista:Water Research
Título revista abreviado:Water Res.
ISSN:00431354
CODEN:WATRA
CAS:4',6 diamidino 2 phenylindole, 47165-04-8; nonylphenol, 25154-52-3; Ethylene Glycols; Fluorescent Dyes; Oligonucleotide Probes; RNA, Ribosomal, 16S; Sewage; Surface-Active Agents; terics, 27986-36-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00431354_v38_n8_p2077_Lozada

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Citas:

---------- APA ----------
Lozada, M., Itria, R.F., Figuerola, E.L.M., Babay, P.A., Gettar, R.T., De Tullio, L.A. & Erijman, L. (2004) . Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge. Water Research, 38(8), 2077-2086.
http://dx.doi.org/10.1016/j.watres.2004.01.032
---------- CHICAGO ----------
Lozada, M., Itria, R.F., Figuerola, E.L.M., Babay, P.A., Gettar, R.T., De Tullio, L.A., et al. "Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge" . Water Research 38, no. 8 (2004) : 2077-2086.
http://dx.doi.org/10.1016/j.watres.2004.01.032
---------- MLA ----------
Lozada, M., Itria, R.F., Figuerola, E.L.M., Babay, P.A., Gettar, R.T., De Tullio, L.A., et al. "Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge" . Water Research, vol. 38, no. 8, 2004, pp. 2077-2086.
http://dx.doi.org/10.1016/j.watres.2004.01.032
---------- VANCOUVER ----------
Lozada, M., Itria, R.F., Figuerola, E.L.M., Babay, P.A., Gettar, R.T., De Tullio, L.A., et al. Bacterial community shifts in nonylphenol polyethoxylates-enriched activated sludge. Water Res. 2004;38(8):2077-2086.
http://dx.doi.org/10.1016/j.watres.2004.01.032