Artículo

Peña Cárcamo, J.R.; Morell, M.L.; Vázquez, C.A.; Vatansever, S.; Upadhyay, A.S.; Överby, A.K.; Cordo, S.M.; García, C.C. "The interplay between viperin antiviral activity, lipid droplets and Junín mammarenavirus multiplication" (2018) Virology. 514:216-229
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Abstract:

Junín arenavirus infections are associated with high levels of interferons in both severe and fatal cases. Upon Junín virus (JUNV) infection a cell signaling cascade initiates, that ultimately attempts to limit viral replication and prevent infection progression through the expression of host antiviral proteins. The interferon stimulated gene (ISG) viperin has drawn our attention as it has been highlighted as an important antiviral protein against several viral infections. The studies of the mechanistic actions of viperin have described important functional domains relating its antiviral and immune-modulating actions through cellular lipid structures. In line with this, through silencing and overexpression approaches, we have identified viperin as an antiviral ISG against JUNV. In addition, we found that lipid droplet structures are modulated during JUNV infection, suggesting its relevance for proper virus multiplication. Furthermore, our confocal microscopy images, bioinformatics and functional results also revealed viperin-JUNV protein interactions that might be participating in this antiviral pathway at lipid droplet level. Altogether, these results will help to better understand the factors mediating innate immunity in arenavirus infection and may lead to the development of pharmacological agents that can boost their effectiveness thereby leading to new treatments for this viral disease. © 2017 Elsevier Inc.

Registro:

Documento: Artículo
Título:The interplay between viperin antiviral activity, lipid droplets and Junín mammarenavirus multiplication
Autor:Peña Cárcamo, J.R.; Morell, M.L.; Vázquez, C.A.; Vatansever, S.; Upadhyay, A.S.; Överby, A.K.; Cordo, S.M.; García, C.C.
Filiación:Laboratorio de Estrategias Antivirales, Departamento de Química Biológica (QB), Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA)- Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Laboratorio de Bioquímica y Biología del virus Junín, QB, FCEyN, UBA-IQUIBICEN, CONICET, Argentina
Graduate School of Science and Engineering, Koc University, Rumelifener Yolu, Istanbul, Sarıyer, Turkey
Department of Clinical Microbiology, Virology, Umeå University, Umeå, Sweden
Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, Umeå, Sweden
Palabras clave:Junín virus; Lipids droplets; Viperin; alpha interferon; fat droplet; viper venom; viral protein; fat droplet; interferon; nucleoprotein; protein; RSAD2 protein, human; animal cell; antiviral activity; Argentine hemorrhagic fever; Article; bioinformatics; cell structure; confocal microscopy; controlled study; disease association; drug mechanism; drug protein binding; gene overexpression; gene silencing; human; human cell; immunomodulation; innate immunity; Junin virus; lipid level; nonhuman; priority journal; protein function; protein interaction; signal transduction; virogenesis; American hemorrhagic fever; chemistry; genetics; immunology; Junin virus; physiology; protein domain; virology; virus replication; Hemorrhagic Fever, American; Humans; Interferons; Junin virus; Lipid Droplets; Nucleoproteins; Protein Domains; Proteins; Virus Replication
Año:2018
Volumen:514
Página de inicio:216
Página de fin:229
DOI: http://dx.doi.org/10.1016/j.virol.2017.10.012
Título revista:Virology
Título revista abreviado:Virology
ISSN:00426822
CODEN:VIRLA
CAS:protein, 67254-75-5; Interferons; Nucleoproteins; Proteins; RSAD2 protein, human
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00426822_v514_n_p216_PenaCarcamo

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Citas:

---------- APA ----------
Peña Cárcamo, J.R., Morell, M.L., Vázquez, C.A., Vatansever, S., Upadhyay, A.S., Överby, A.K., Cordo, S.M.,..., García, C.C. (2018) . The interplay between viperin antiviral activity, lipid droplets and Junín mammarenavirus multiplication. Virology, 514, 216-229.
http://dx.doi.org/10.1016/j.virol.2017.10.012
---------- CHICAGO ----------
Peña Cárcamo, J.R., Morell, M.L., Vázquez, C.A., Vatansever, S., Upadhyay, A.S., Överby, A.K., et al. "The interplay between viperin antiviral activity, lipid droplets and Junín mammarenavirus multiplication" . Virology 514 (2018) : 216-229.
http://dx.doi.org/10.1016/j.virol.2017.10.012
---------- MLA ----------
Peña Cárcamo, J.R., Morell, M.L., Vázquez, C.A., Vatansever, S., Upadhyay, A.S., Överby, A.K., et al. "The interplay between viperin antiviral activity, lipid droplets and Junín mammarenavirus multiplication" . Virology, vol. 514, 2018, pp. 216-229.
http://dx.doi.org/10.1016/j.virol.2017.10.012
---------- VANCOUVER ----------
Peña Cárcamo, J.R., Morell, M.L., Vázquez, C.A., Vatansever, S., Upadhyay, A.S., Överby, A.K., et al. The interplay between viperin antiviral activity, lipid droplets and Junín mammarenavirus multiplication. Virology. 2018;514:216-229.
http://dx.doi.org/10.1016/j.virol.2017.10.012