Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

In the context of pathogenesis of HBV infection, HBV genotypes and mutants have been shown to affect the natural course of chronic infection and treatment outcomes. In this work, we studied the induction of apoptosis by the replication of HBV subgenotypes F1b and F4, and the naturally occurring mutants BCP and preCore. Both subgenotypes F1b and F4 HBV genome transfections induced cell death by apoptosis in human hepatocytes. The BCPdm (A1762T/G1764A) and preCore (G1896A) mutants induced higher levels of apoptosis than the wt virus. This increase in apoptosis was not associated with the enhanced viral replication of the variants. HBV-mediated apoptosis was independent of viral subgenotypes, and associated with the modulation of members of the regulatory Bcl-2 family proteins expression in the mitochondrial apoptotic pathway. Finally, the apoptosis induction increase observed for the preCore mutants suggests that HBeAg might have an anti-apoptotic effect in human hepatocytes. © 2017 Elsevier Inc.

Registro:

Documento: Artículo
Título:Human hepatocytes apoptosis induced by replication of hepatitis B virus subgenotypes F1b and F4: Role of basal core promoter and preCore mutations
Autor:Elizalde, M.M.; Sevic, I.; González López Ledesma, M.M.; Campos, R.H.; Barbini, L.; Flichman, D.M.
Filiación:Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Microbiología, Inmunología y Biotecnología, Cátedra de Virología, Buenos Aires, Argentina
Universidad Nacional de Mar del Plata, Facultad de Ciencias Exactas y Naturales, Departamento de Química, Mar del Plata, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Palabras clave:Apoptosis; Basal core promoter mutations; Hepatitis B virus; Pathogenesis; PreCore mutation; Subgenotype F1b; Subgenotype F4; caspase 3; hepatitis B(e) antigen; protein Bax; protein bcl 2; protein bcl x; hepatitis B core antigen; antigen expression; apoptosis; Article; basal core gene; cell adhesion; cell contact; cell viability; controlled study; down regulation; enzyme activity; gene expression; Hepatitis B virus; Hepatitis B virus subtype F1b; Hepatitis B virus subtype F4; human; human cell; liver cell; mitochondrion; nonhuman; preCore gene; priority journal; promoter region; protein expression; virus gene; virus replication; cell line; genetics; Hepatitis B virus; liver cell; mutation; physiology; promoter region; virology; Apoptosis; Cell Line; Hepatitis B Core Antigens; Hepatitis B virus; Hepatocytes; Humans; Mutation; Promoter Regions, Genetic; Virus Replication
Año:2018
Volumen:513
Página de inicio:160
Página de fin:167
DOI: http://dx.doi.org/10.1016/j.virol.2017.10.016
Título revista:Virology
Título revista abreviado:Virology
ISSN:00426822
CODEN:VIRLA
CAS:caspase 3, 169592-56-7; protein bcl 2, 219306-68-0; Hepatitis B Core Antigens
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00426822_v513_n_p160_Elizalde

Referencias:

  • Adams, J.M., Ways of dying: multiple pathways to apoptosis (2003) Genes Dev., 17, pp. 2481-2495
  • Baumert, T.F., Thimme, R., Weizsäcker, F.V., Pathogenesis of hepatitis B virus infection (2007) World J. Gastroenterol., 13, pp. 82-90
  • Bhoola, N.H., Kramvis, A., Hepatitis B e antigen expression by hepatitis B virus subgenotype A1 relative to subgenotypes A2 and D3 in cultured hepatocellular carcinoma (Huh7) cells (2016) Intervirology, 59, pp. 48-59
  • Buckwold, V.E., Xu, Z., Chen, M., Yen, T.S., Ou, J.H., Effects of a naturally occurring mutation in the hepatitis B virus basal core promoter on precore gene expression and viral replication (1996) J. Virol., 70, pp. 5845-5851
  • Chen, C.-J., Yang, H.-I., Natural history of chronic hepatitis B REVEALed (2011) J. Gastroenterol. Hepatol., 26, pp. 628-638
  • Chen, G.G., Lai, P.B.S., Chan, P.K.S., Chak, E.C.W., Yip, J.H.Y., Ho, R.L.K., Leung, B.C.S., Lau, W.Y., Decreased expression of Bid in human hepatocellular carcinoma is related to hepatitis B virus X protein (2001) Eur. J. Cancer, 37, pp. 1695-1702
  • Ching, L.K., Gounder, P.P., Bulkow, L., Spradling, P.R., Bruce, M.G., Negus, S., Snowball, M., McMahon, B.J., Incidence of hepatocellular carcinoma according to hepatitis B virus genotype in Alaska Native people (2016) Liver Int., 36, pp. 1507-1515
  • Chipuk, J.E., Moldoveanu, T., Llambi, F., Parsons, M.J., Green, D.R., The BCL-2 family reunion (2010) Mol. Cell, 37, pp. 299-310
  • Chirillo, P., Pagano, S., Natoli, G., Puri, P.L., Burgio, V.L., Balsano, C., Levrero, M., The hepatitis B virus X gene induces p53-mediated programmed cell death (1997) Proc. Natl. Acad. Sci. USA, 94, pp. 8162-8167
  • Chisari, F.V., Isogawa, M., Wieland, S.F., Pathogenesis of hepatitis B virus infection (2010) Pathol. Biol., 58, pp. 258-266
  • Ehrmann, J., Galuszkova, D., Ek, B.V., Murray, P.G., Kolao, Z., Apoptosis-related proteins, BCL-2, BAX, FAS, FAS-L and PCNA in liver biopsies of patients with chronic Hepatitis B virus infection (2000) Pathol. Oncol. Res., 6, pp. 130-135
  • Gounder, P.P., Bulkow, L.R., Snowball, M., Negus, S., Spradling, P.R., McMahon, B.J., Hepatocellular carcinoma risk in Alaska native children and young adults with hepatitis B virus: retrospective cohort analysis (2016) J. Pediatr., 178, pp. 206-213
  • Iavarone, M., Colombo, M., HBV infection and hepatocellular carcinoma (2013) Clin. Liver Dis., 17, pp. 375-397
  • Jammeh, S., Tavner, F., Watson, R., Thomas, H.C., Karayiannis, P., Effect of basal core promoter and pre-core mutations on hepatitis B virus replication (2008) J. Gen. Virol., 89, pp. 901-909
  • Jegaskanda, S., Ahn, S.H., Skinner, N., Thompson, A.J., Ngyuen, T., Holmes, J., De Rose, R., Revill, P.A., Downregulation of interleukin-18-mediated cell signaling and interferon gamma expression by the hepatitis B virus e antigen (2014) J. Virol., 88, pp. 10412-10420
  • Kanda, T., Yokosuka, O., Imazeki, F., Yamada, Y., Imamura, T., Fukai, K., Nagao, K., Saisho, H., Hepatitis B virus X protein (HBx)‐induced apoptosis in HuH‐7 cells: influence of HBV genotype and basal core promoter mutations (2004) Scand. J. Gastroenterol., 39, pp. 478-485
  • Kao, J.H., Chen, P.J., Lai, M.Y., Chen, D.S., Basal core promoter mutations of hepatitis B virus increase the risk of hepatocellular carcinoma in hepatitis B carriers (2003) Gastroenterology, 124, pp. 327-334
  • Kidd-Ljunggren, K., Öberg, M., Kidd, A.H., Hepatitis B virus X gene 1751 to 1764 mutations: implications for HBeAg status and disease (1997) J. Gen. Virol., 78, pp. 1469-1478
  • Kim, H.J., Kim, S.Y., Kim, J., Lee, H., Choi, M., Kim, J.K., Ahn, J.K., Hepatitis B virus X protein induces apoptosis by enhancing translocation of Bax to mitochondria (2008) IUBMB Life, 60, pp. 473-480
  • Kramvis, A., Genotypes and genetic variability of hepatitis B virus (2014) Intervirology, 57, pp. 141-150
  • Lamontagne, R.J., Bagga, S., Bouchard, M.J., Hepatitis B virus molecular biology and pathogenesis (2016) Hepatoma Res., 2, p. 163
  • Lang, T., Lo, C., Skinner, N., Locarnini, S., Visvanathan, K., Mansell, A., The Hepatitis B e antigen (HBeAg) targets and suppresses activation of the Toll-like receptor signaling pathway (2011) J. Hepatol., 55, pp. 762-769
  • Ledesma, M.M.G.L., Mojsiejczuk, L.N., Rodrigo, B., Sevic, I., Mammana, L., Galdame, O., Gadano, A., Flichman, D., Hepatitis B virus genotype distribution and genotype-specific BCP/precore substitutions in acute and chronic infections in Argentina (2015) PLoS One, 10, pp. 1-16
  • Lin, C.L., Liao, L.Y., Wang, C.S., Chen, P.J., Lai, M.Y., Chen, D.S., Kao, J.H., Basal core-promoter mutant of hepatitis B virus and progression of liver disease in hepatitis B e antigen-negative chronic hepatitis B (2005) Liver Int., 25, pp. 564-570
  • Lin, C.-L., Kao, J.-H., The clinical implications of hepatitis B virus genotype: recent advances (2011) J. Gastroenterol. Hepatol., 26, pp. 123-130
  • Lin, C.-L., Kao, J.-H., Hepatitis B virus genotypes and variants (2015) Cold Spring Harb. Perspect. Med., 5. , (a021436–a021436)
  • Liu, C.J., Kao, J.H., Global perspective on the natural history of chronic hepatitis B: role of hepatitis B virus genotypes A to J (2013) Semin Liver Dis., 33, pp. 97-102
  • Liu, C.J., Chen, P.J., Lai, M.Y., Kao, J.H., Chen, D.S., Evolution of precore/core promoter mutations in hepatitis B carriers with hepatitis B e antigen seroreversion (2004) J. Med. Virol., 74, pp. 237-245
  • Liu, C.J., Cheng, H.R., Chen, C.L., Chen, T.C., Tseng, T.C., Wang, Z.L., Chen, P.J., Kao, J.H., Effects of hepatitis B virus precore and basal core promoter mutations on the expression of viral antigens: genotype B vs C (2011) J. Viral Hepat., 18, pp. 482-490
  • Liu, C.-J., Kao, J.-H., Hepatitis B virus-related hepatocellular carcinoma: epidemiology and pathogenic role of viral factors (2007) J. Chin. Med. Assoc., 70, pp. 141-145
  • Liu, D., Cui, L., Wang, Y., Yang, G., He, J., Hao, R., Fan, C., Guo, D., Hepatitis B e antigen and its precursors promote the progress of hepatocellular carcinoma by interacting with NUMB and decreasing p53 activity (2016) Hepatology, 64, pp. 390-404
  • Livingston, S.E., Simonetti, J.P., McMahon, B.J., Bulkow, L.R., Hurlburt, K.J., Homan, C.E., Snowball, M.M., Chulanov, V.P., Hepatitis B virus genotypes in Alaska Native people with hepatocellular carcinoma: preponderance of genotype F (2007) J. Infect. Dis., 195, pp. 5-11
  • Lu, Y., Tan, T., Zhang, J., Chen, W., Cellular apoptosis induced by replication of hepatitis B virus: possible link between viral genotype and clinical outcome (2007) Virol. J., 4, p. 117
  • Lu, Y.W., Chen, W.N., Human hepatitis B virus X protein induces apoptosis in HepG2 cells: role of BH3 domain (2005) Biochem. Biophys. Res. Commun., 338, pp. 1551-1556
  • Lu, Y.W., Tan, T.L., Chan, V., Chen, W.N., The HBSP gene is expressed during HBV replication, and its coded BH3-containing spliced viral protein induces apoptosis in HepG2 cells (2006) Biochem. Biophys. Res. Commun., 351, pp. 64-70
  • McMahon, B.J., The natural history of chronic hepatitis B virus infection (2009) Hepatology, 49, pp. 45-55
  • Miao, J., Chen, G.G., Chun, S., Lai, P.P.S., Hepatitis B virus X protein induces apoptosis in hepatoma cells through inhibiting Bcl-xL expression (2006) Cancer Lett., 236, pp. 115-124
  • Milich, D., Liang, T.J., Exploring the biological basis of hepatitis B e antigen in hepatitis B virus infection (2003) Hepatology, 38, pp. 1075-1086
  • Moriyama, K., Okamoto, H., Tsuda, F., Mayumi, M., Reduced precore transcription and enhanced core-pregenome transcription of hepatitis B virus DNA after replacement of the precore-core promoter with sequences associated with e antigen-seronegative persistent infections (1996) Virology, 226, pp. 269-280
  • Murakami, S., Hepatitis B virus X protein: a multifunctional viral regulator (2001) J. Gastroenterol., 36, pp. 651-660
  • Parekh, S., Zoulim, F., Ahn, S.H., Tsai, A., Li, J., Kawai, S., Khan, N., Tong, S., Genome replication, virion secretion, and e antigen expression of naturally occurring hepatitis B virus core promoter mutants (2003) J. Virol., 77, pp. 6601-6612
  • Pezzano, S.C., Torres, C., Fainboim, H.A., Bouzas, M.B., Schroder, T., Giuliano, S.F., Paz, S., Mbayed, V.A., Hepatitis B virus in Buenos Aires, Argentina: genotypes, virological characteristics and clinical outcomes (2011) Clin. Microbiol. Infect., 17, pp. 223-231
  • Pollicino, T., Saitta, C., Raimondo, G., Hepatocellular carcinoma: the point of view of the hepatitis B virus (2011) Carcinogenesis, 32, pp. 1122-1132
  • Rawat, S., Clippinger, A.J., Bouchard, M.J., Modulation of apoptotic signaling by the Hepatitis B Virus X protein (2012) Viruses, 4, pp. 2945-2972
  • Schattenberg, J.M., Schuchmann, M., Galle, P.R., Cell death and hepatocarcinogenesis: dysregulation of apoptosis signaling pathways (2011) J. Gastroenterol. Hepatol., 26, pp. 213-219
  • Sevic, I., González López Ledesma, M.M., Flichman, D.M., Campos, H.R., HBV DNA genome co-transfection procedure for the evaluation of relative fitness (2017) PLoS One, 12 (5), pp. 1-12
  • Siddiqui, W.A., Ahad, A., Ahsan, H., The mystery of BCL2 family: Bcl-2 proteins and apoptosis: an update (2015) Arch. Toxicol., 89, pp. 289-317
  • Sunbul, M., Hepatitis B virus genotypes: global distribution and clinical importance (2014) World J. Gastroenterol., 20, pp. 5427-5434
  • Tong, S., Revill, P., Overview of hepatitis B viral replication and genetic variability (2016) J. Hepatol., 64, pp. S4-S16
  • Tong, S., Kim, K.H., Chante, C., Wands, J., Li, J., Hepatitis B virus e antigen variants (2005) Int. J. Med. Sci., 2, pp. 2-7
  • Wei, F., Zheng, Q., Li, M., Wu, M., The association between hepatitis B mutants and hepatocellular carcinoma (2017) Medicine, 96, p. e6835
  • (2017), WHO | Hepatitis B. WHO; Wu, S., Kanda, T., Imazeki, F., Arai, M., Yonemitsu, Y., Nakamoto, S., Fujiwara, K., Yokosuka, O., Hepatitis B virus e antigen downregulates cytokine production in human hepatoma cell lines (2010) Viral Immunol., 23, pp. 467-476
  • Yang, Z., Zhuang, L., Lu, Y., Xu, Q., Tang, B., Chen, X., Naturally occurring basal core promoter A1762T/G1764A dual mutations increase the risk of HBV-related hepatocellular carcinoma: a meta-analysis (2016) Oncotarget, 7, pp. 12525-12536
  • Yeganeh, B., Rezaei Moghadam, A., Alizadeh, J., Wiechec, E., Alavian, S.M., Hashemi, M., Geramizadeh, B., Ghavami, S., Hepatitis B and C virus-induced hepatitis: apoptosis, autophagy, and unfolded protein response (2015) World J. Gastroenterol., 21, pp. 13225-13239
  • Zhang, Z.H., Wu, C.C., Chen, X.W., Li, X., Li, J., Lu, M.J., Genetic variation of hepatitis B virus and its significance for pathogenesis (2016) World J. Gastroenterol., 22, pp. 126-144

Citas:

---------- APA ----------
Elizalde, M.M., Sevic, I., González López Ledesma, M.M., Campos, R.H., Barbini, L. & Flichman, D.M. (2018) . Human hepatocytes apoptosis induced by replication of hepatitis B virus subgenotypes F1b and F4: Role of basal core promoter and preCore mutations. Virology, 513, 160-167.
http://dx.doi.org/10.1016/j.virol.2017.10.016
---------- CHICAGO ----------
Elizalde, M.M., Sevic, I., González López Ledesma, M.M., Campos, R.H., Barbini, L., Flichman, D.M. "Human hepatocytes apoptosis induced by replication of hepatitis B virus subgenotypes F1b and F4: Role of basal core promoter and preCore mutations" . Virology 513 (2018) : 160-167.
http://dx.doi.org/10.1016/j.virol.2017.10.016
---------- MLA ----------
Elizalde, M.M., Sevic, I., González López Ledesma, M.M., Campos, R.H., Barbini, L., Flichman, D.M. "Human hepatocytes apoptosis induced by replication of hepatitis B virus subgenotypes F1b and F4: Role of basal core promoter and preCore mutations" . Virology, vol. 513, 2018, pp. 160-167.
http://dx.doi.org/10.1016/j.virol.2017.10.016
---------- VANCOUVER ----------
Elizalde, M.M., Sevic, I., González López Ledesma, M.M., Campos, R.H., Barbini, L., Flichman, D.M. Human hepatocytes apoptosis induced by replication of hepatitis B virus subgenotypes F1b and F4: Role of basal core promoter and preCore mutations. Virology. 2018;513:160-167.
http://dx.doi.org/10.1016/j.virol.2017.10.016