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In the context of pathogenesis of HBV infection, HBV genotypes and mutants have been shown to affect the natural course of chronic infection and treatment outcomes. In this work, we studied the induction of apoptosis by the replication of HBV subgenotypes F1b and F4, and the naturally occurring mutants BCP and preCore. Both subgenotypes F1b and F4 HBV genome transfections induced cell death by apoptosis in human hepatocytes. The BCPdm (A1762T/G1764A) and preCore (G1896A) mutants induced higher levels of apoptosis than the wt virus. This increase in apoptosis was not associated with the enhanced viral replication of the variants. HBV-mediated apoptosis was independent of viral subgenotypes, and associated with the modulation of members of the regulatory Bcl-2 family proteins expression in the mitochondrial apoptotic pathway. Finally, the apoptosis induction increase observed for the preCore mutants suggests that HBeAg might have an anti-apoptotic effect in human hepatocytes. © 2017 Elsevier Inc.


Documento: Artículo
Título:Human hepatocytes apoptosis induced by replication of hepatitis B virus subgenotypes F1b and F4: Role of basal core promoter and preCore mutations
Autor:Elizalde, M.M.; Sevic, I.; González López Ledesma, M.M.; Campos, R.H.; Barbini, L.; Flichman, D.M.
Filiación:Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Microbiología, Inmunología y Biotecnología, Cátedra de Virología, Buenos Aires, Argentina
Universidad Nacional de Mar del Plata, Facultad de Ciencias Exactas y Naturales, Departamento de Química, Mar del Plata, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Palabras clave:Apoptosis; Basal core promoter mutations; Hepatitis B virus; Pathogenesis; PreCore mutation; Subgenotype F1b; Subgenotype F4; caspase 3; hepatitis B(e) antigen; protein Bax; protein bcl 2; protein bcl x; hepatitis B core antigen; antigen expression; apoptosis; Article; basal core gene; cell adhesion; cell contact; cell viability; controlled study; down regulation; enzyme activity; gene expression; Hepatitis B virus; Hepatitis B virus subtype F1b; Hepatitis B virus subtype F4; human; human cell; liver cell; mitochondrion; nonhuman; preCore gene; priority journal; promoter region; protein expression; virus gene; virus replication; cell line; genetics; Hepatitis B virus; liver cell; mutation; physiology; promoter region; virology; Apoptosis; Cell Line; Hepatitis B Core Antigens; Hepatitis B virus; Hepatocytes; Humans; Mutation; Promoter Regions, Genetic; Virus Replication
Página de inicio:160
Página de fin:167
Título revista:Virology
Título revista abreviado:Virology
CAS:caspase 3, 169592-56-7; protein bcl 2, 219306-68-0; Hepatitis B Core Antigens


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---------- APA ----------
Elizalde, M.M., Sevic, I., González López Ledesma, M.M., Campos, R.H., Barbini, L. & Flichman, D.M. (2018) . Human hepatocytes apoptosis induced by replication of hepatitis B virus subgenotypes F1b and F4: Role of basal core promoter and preCore mutations. Virology, 513, 160-167.
---------- CHICAGO ----------
Elizalde, M.M., Sevic, I., González López Ledesma, M.M., Campos, R.H., Barbini, L., Flichman, D.M. "Human hepatocytes apoptosis induced by replication of hepatitis B virus subgenotypes F1b and F4: Role of basal core promoter and preCore mutations" . Virology 513 (2018) : 160-167.
---------- MLA ----------
Elizalde, M.M., Sevic, I., González López Ledesma, M.M., Campos, R.H., Barbini, L., Flichman, D.M. "Human hepatocytes apoptosis induced by replication of hepatitis B virus subgenotypes F1b and F4: Role of basal core promoter and preCore mutations" . Virology, vol. 513, 2018, pp. 160-167.
---------- VANCOUVER ----------
Elizalde, M.M., Sevic, I., González López Ledesma, M.M., Campos, R.H., Barbini, L., Flichman, D.M. Human hepatocytes apoptosis induced by replication of hepatitis B virus subgenotypes F1b and F4: Role of basal core promoter and preCore mutations. Virology. 2018;513:160-167.