Artículo

Benencia, F.; Gamba, G.; Cavalieri, H.; Courreges, M.C.; Benedetti, R.; Villamil, S.M.; Massouh, E.J. "Nitric oxide and HSV vaginal infection in BALB/c mice" (2003) Virology. 309(1):75-84
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Abstract:

Here we study the role of nitric oxide in the vaginal infection of Balb/c mice with herpes simplex virus type 2. Inducible nitric oxide synthase (iNOS) mRNA was detected by RT-PCR in vaginal tissue and inguinal lymph nodes early postinfection. iNOS was also found to be activated in cells recovered from vaginal washings of infected animals. Animals treated with aminoguanidine (AG), an iNOS inhibitor, showed a dose-dependent increase in vaginal pathology after viral infection compared to controls. Viral titers in vaginal washings and vaginas were higher in AG-treated mice. Treated animals presented higher PMN counts in vaginal washings compared to controls. Histopathology studies revealed a profound inflammatory exudate in vaginal tissue of treated animals. Finally, RT-PCR analysis showed increased expression of the chemokines MIP-2 and RANTES in vaginal tissue and inguinal lymph nodes of these animals. © 2003 Elsevier Science (USA). All rights reserved.

Registro:

Documento: Artículo
Título:Nitric oxide and HSV vaginal infection in BALB/c mice
Autor:Benencia, F.; Gamba, G.; Cavalieri, H.; Courreges, M.C.; Benedetti, R.; Villamil, S.M.; Massouh, E.J.
Filiación:Department of Biological Chemistry, University of Buenos Aires, Ciudad Universitaria, (1428) Buenos Aires, Argentina
Department of Pathology, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina
CRRWH, University of Pennsylvania, 421 Curie Boulevard, Philadelphia, PA 19104, United States
Palabras clave:aminoguanidine; macrophage inflammatory protein 2; messenger RNA; n acetyl s nitrosopenicillamine; nitric oxide; nitric oxide donor; nitric oxide synthase; nitric oxide synthase inhibitor; RANTES; animal cell; animal experiment; animal model; animal tissue; article; cell count; controlled study; enzyme activation; exudate; female; Herpes simplex virus 2; histopathology; inguinal lymph node; mouse; nonhuman; polymorphonuclear cell; priority journal; protein expression; reverse transcription polymerase chain reaction; vaginitis; virus replication; virus titration; Animalia; Human herpesvirus 2; Simplexvirus
Año:2003
Volumen:309
Número:1
Página de inicio:75
Página de fin:84
DOI: http://dx.doi.org/10.1016/S0042-6822(02)00057-0
Título revista:Virology
Título revista abreviado:Virology
ISSN:00426822
CODEN:VIRLA
CAS:aminoguanidine, 1068-42-4, 2582-30-1, 79-17-4; n acetyl s nitrosopenicillamine, 79032-48-7; nitric oxide, 10102-43-9; nitric oxide synthase, 125978-95-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00426822_v309_n1_p75_Benencia

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Citas:

---------- APA ----------
Benencia, F., Gamba, G., Cavalieri, H., Courreges, M.C., Benedetti, R., Villamil, S.M. & Massouh, E.J. (2003) . Nitric oxide and HSV vaginal infection in BALB/c mice. Virology, 309(1), 75-84.
http://dx.doi.org/10.1016/S0042-6822(02)00057-0
---------- CHICAGO ----------
Benencia, F., Gamba, G., Cavalieri, H., Courreges, M.C., Benedetti, R., Villamil, S.M., et al. "Nitric oxide and HSV vaginal infection in BALB/c mice" . Virology 309, no. 1 (2003) : 75-84.
http://dx.doi.org/10.1016/S0042-6822(02)00057-0
---------- MLA ----------
Benencia, F., Gamba, G., Cavalieri, H., Courreges, M.C., Benedetti, R., Villamil, S.M., et al. "Nitric oxide and HSV vaginal infection in BALB/c mice" . Virology, vol. 309, no. 1, 2003, pp. 75-84.
http://dx.doi.org/10.1016/S0042-6822(02)00057-0
---------- VANCOUVER ----------
Benencia, F., Gamba, G., Cavalieri, H., Courreges, M.C., Benedetti, R., Villamil, S.M., et al. Nitric oxide and HSV vaginal infection in BALB/c mice. Virology. 2003;309(1):75-84.
http://dx.doi.org/10.1016/S0042-6822(02)00057-0