Vero cell cultures persistently infected with the arenaviruses Junin, Pichinde, Tacaribe, and Tamiami were established and designated Vero-Jun, Vero-Pic, Vero-Tac, and Vero-Tam, respectively. Two types of carrier cultures could be easily distinguished: Vero-Jun and Vero-Tac systems were characterized by a lack of infectious virus production after a few cell transfers, whereas a more productive state with continuous release of virus was observed in Vero-Pic and Vero-Tam cultures. These differences appeared to be related to resistance of the culture to viral superinfection. In fact, Vero-Jun and Vero-Tac cultures totally excluded only the replication of the serologically more closely related arenaviruses Amapari, Junin, or Tacaribe, while the refractoriness of Vero-Pic and Vero-Tam cultures was extended to most of the virus group members. The resistance of Vero-Jun cells to superinfection by Junin or Tacaribe virus could be ascribed to the production of specific uv-resistant Junin interfering particles, which showed a specific range of interference against Junin and Tacaribe viruses. Interfering particles against homotypic and heterotypic arenaviruses were isolated from Vero-Pic cultures. However, the degree of interference developed by these Pic-interfering particles was not enough to fully explain reinfecting virus exclusion from Vero-Pic cultures. Viral susceptibility of persistent cultures is proposed as a useful tool to examine relationships of members of the arenavirus group. © 1983.
Documento: | Artículo |
Título: | Response of cells persistently infected with arenaviruses to superinfection with homotypic and heterotypic viruses |
Autor: | Damonte, E.B.; Mersich, S.E.; Coto, C.E. |
Filiación: | Cátedra de Virologiá, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina |
Palabras clave: | arenavirus; cell culture; in vitro study; nonhuman; persistent infection; virus infection; virus interference; Animal; Arenaviridae; Arenaviruses, New World; Cell Line; Cercopithecus aethiops; Comparative Study; Plaque Assay; Support, Non-U.S. Gov't; Ultraviolet Rays; Viral Interference |
Año: | 1983 |
Volumen: | 129 |
Número: | 2 |
Página de inicio: | 474 |
Página de fin: | 478 |
DOI: | http://dx.doi.org/10.1016/0042-6822(83)90185-X |
Título revista: | Virology |
Título revista abreviado: | Virology |
ISSN: | 00426822 |
CODEN: | VIRLA |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00426822_v129_n2_p474_Damonte |