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Abstract:

Neuropeptide Y (NPY) and orexin are neuropeptides involved in the regulation of feeding in vertebrates. In this study we determined the NPY and orexin mRNA tissue expression and their immunoreactivity distribution in both preoptic area and hypothalamus, regions involved in the regulation of feeding behavior. Both peptides presented a wide expression in all tissues examined. The NPY-immunoreactive (ir) cells were localized in the ventral nucleus posterioris periventricularis (NPPv) and numerous ir-NPY fibers were found in the nucleus lateralis tuberis (NLT), the nucleus recess lateralis (NRL) and the neurohypophysis. Ir-orexin cells were observed in the NPPv, dorsal NLT, ventral NLT, lateral NLT (NLTl) and the lateral NRL. Ir-orexin fibers were widespread distributed along all the hypothalamus, especially in the NLTl. Additionally, we observed the presence of ir-orexin immunostaining in adenohypophyseal cells, especially in somatotroph cells and the presence of a few ir-orexin-A fibers in the neurohypophysis. In conclusion, both peptides have an ubiquitous mRNA tissue expression and are similarly distributed in the hypothalamus and preoptic area of Cichlasoma dimerus. The presence of ir-orexin in adenohypohyseal cells and the presence of ir-orexin and NPY fibers in the neurohypophysis suggest that both peptides may play an important neuroendocrine role in anterior pituitary. © 2013 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Orexin and neuropeptide Y: Tissue specific expression and immunoreactivity in the hypothalamus and preoptic area of the cichlid fish Cichlasoma dimerus
Autor:Pérez Sirkin, D.I.; Suzuki, H.; Cánepa, M.M.; Vissio, P.G.
Filiación:Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, Dpto. de Biodiversidad y Biología Experimental, Ciudad Universitaria, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EHA Buenos Aires, Argentina
IBBEA, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
Department of Biology, Fukuoka University of Education, Akamabunkyo-machi 1-1, Munakata, Fukuoka 811-4192, Japan
Palabras clave:Brain; Fish; NPY; Orexin; Pituitary; messenger RNA; neuropeptide Y; orexin; adenohypophysis; animal cell; animal tissue; article; behavior control; brain tissue; cellular distribution; Cichlasoma dimerus; cichlid; controlled study; endocrine function; feeding behavior; female; fish; hypothalamus; hypothalamus periventricular nucleus; immunocompetent cell; immunohistochemistry; immunoreactivity; male; nerve fiber; neurohypophysis; nonhuman; nucleotide sequence; preoptic area; protein analysis; protein expression; protein function; protein localization; thalamus ventral nucleus; tuberal nucleus; Cichlasoma; Cichlidae; Vertebrata; Brain; Fish; NPY; Orexin; Pituitary; Animals; Cichlids; Hypothalamus; Intracellular Signaling Peptides and Proteins; Neuropeptide Y; Neuropeptides; Preoptic Area
Año:2013
Volumen:45
Número:6
Página de inicio:452
Página de fin:459
DOI: http://dx.doi.org/10.1016/j.tice.2013.09.001
Título revista:Tissue and Cell
Título revista abreviado:Tissue Cell
ISSN:00408166
CODEN:TICEB
CAS:neuropeptide Y, 82785-45-3, 83589-17-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00408166_v45_n6_p452_PerezSirkin

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Citas:

---------- APA ----------
Pérez Sirkin, D.I., Suzuki, H., Cánepa, M.M. & Vissio, P.G. (2013) . Orexin and neuropeptide Y: Tissue specific expression and immunoreactivity in the hypothalamus and preoptic area of the cichlid fish Cichlasoma dimerus. Tissue and Cell, 45(6), 452-459.
http://dx.doi.org/10.1016/j.tice.2013.09.001
---------- CHICAGO ----------
Pérez Sirkin, D.I., Suzuki, H., Cánepa, M.M., Vissio, P.G. "Orexin and neuropeptide Y: Tissue specific expression and immunoreactivity in the hypothalamus and preoptic area of the cichlid fish Cichlasoma dimerus" . Tissue and Cell 45, no. 6 (2013) : 452-459.
http://dx.doi.org/10.1016/j.tice.2013.09.001
---------- MLA ----------
Pérez Sirkin, D.I., Suzuki, H., Cánepa, M.M., Vissio, P.G. "Orexin and neuropeptide Y: Tissue specific expression and immunoreactivity in the hypothalamus and preoptic area of the cichlid fish Cichlasoma dimerus" . Tissue and Cell, vol. 45, no. 6, 2013, pp. 452-459.
http://dx.doi.org/10.1016/j.tice.2013.09.001
---------- VANCOUVER ----------
Pérez Sirkin, D.I., Suzuki, H., Cánepa, M.M., Vissio, P.G. Orexin and neuropeptide Y: Tissue specific expression and immunoreactivity in the hypothalamus and preoptic area of the cichlid fish Cichlasoma dimerus. Tissue Cell. 2013;45(6):452-459.
http://dx.doi.org/10.1016/j.tice.2013.09.001