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Abstract:

Brain-derived neurotrophic factor (BDNF) is a neurotrophin involved in the development and maintenance of vertebrate nervous systems. Although there were several studies in classical animal models, scarce information for fish was available. The main purpose of this study was to analyze the distribution of BDNF in the brain and retina of the cichlid fish Cichlasoma dimerus. By immunohistochemistry we detected BDNF-like immunoreactive cells in the cytoplasm and the nuclei of the ganglion cell layer and the inner nuclear layer of the retina. In the optic tectum, BDNF-like immunoreactivity was detected in the nucleus of neurons of the stratum periventriculare and the stratum marginale and in neurons of the intermediate layers. In the hypothalamus we found BDNF-like immunoreactivity mainly in the cytoplasm of the nucleus lateralis tuberis and the nucleus of the lateral recess. To confirm the nuclear and cytoplasm localization of BDNF we performed subcellular fractionation, followed by Western blot, detecting a 39 kDa immunoreactive-band corresponding to a possible precursor form of BDNF in both fractions. BDNF-like immunoreactivity was distributed in areas related with photoreception (retina), the integration center of retinal projections (optic tectum) and the control center of background and stress adaptation (hypothalamus). These results provide baseline anatomical information for future research about the role of neurotrophins in the adult fish central nervous system. © 2008 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Brain-derived neurotrophic factor (BDNF)-like immunoreactivity localization in the retina and brain of Cichlasoma dimerus (Teleostei, Perciformes)
Autor:Vissio, P.G.; Cánepa, M.M.; Maggese, M.C.
Filiación:CONICET, Laboratorio de Embriología Animal, DBBE, Ciudad Universitaria, (C1428EHA) Buenos Aires, Argentina
Palabras clave:Hypothalamus; Neurotrophins; Optic tectum; Retina; Teleost; brain derived neurotrophic factor; neurotrophin; animal cell; animal tissue; article; brain region; cell fractionation; cell nucleus; central nervous system; cichlid; cytoplasm; fish; hypothalamus; hypothalamus nucleus; immunocompetent cell; immunoreactivity; medical research; nerve cell; nervous system development; nonhuman; optic tectum; Perciformes; protein localization; retina; stress; teleost; vertebrate; Western blotting; Animalia; Cichlasoma; Cichlidae; Perciformes; Teleostei; Vertebrata
Año:2008
Volumen:40
Número:4
Página de inicio:261
Página de fin:270
DOI: http://dx.doi.org/10.1016/j.tice.2008.01.001
Título revista:Tissue and Cell
Título revista abreviado:Tissue Cell
ISSN:00408166
CODEN:TICEB
CAS:brain derived neurotrophic factor, 218441-99-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00408166_v40_n4_p261_Vissio

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Citas:

---------- APA ----------
Vissio, P.G., Cánepa, M.M. & Maggese, M.C. (2008) . Brain-derived neurotrophic factor (BDNF)-like immunoreactivity localization in the retina and brain of Cichlasoma dimerus (Teleostei, Perciformes). Tissue and Cell, 40(4), 261-270.
http://dx.doi.org/10.1016/j.tice.2008.01.001
---------- CHICAGO ----------
Vissio, P.G., Cánepa, M.M., Maggese, M.C. "Brain-derived neurotrophic factor (BDNF)-like immunoreactivity localization in the retina and brain of Cichlasoma dimerus (Teleostei, Perciformes)" . Tissue and Cell 40, no. 4 (2008) : 261-270.
http://dx.doi.org/10.1016/j.tice.2008.01.001
---------- MLA ----------
Vissio, P.G., Cánepa, M.M., Maggese, M.C. "Brain-derived neurotrophic factor (BDNF)-like immunoreactivity localization in the retina and brain of Cichlasoma dimerus (Teleostei, Perciformes)" . Tissue and Cell, vol. 40, no. 4, 2008, pp. 261-270.
http://dx.doi.org/10.1016/j.tice.2008.01.001
---------- VANCOUVER ----------
Vissio, P.G., Cánepa, M.M., Maggese, M.C. Brain-derived neurotrophic factor (BDNF)-like immunoreactivity localization in the retina and brain of Cichlasoma dimerus (Teleostei, Perciformes). Tissue Cell. 2008;40(4):261-270.
http://dx.doi.org/10.1016/j.tice.2008.01.001