Abstract:
The glass transition temperature of whey proteins concentrate (WPC)/hydroxypropyl methylcellulose (HPMC) co-dried mixtures with different degrees of phase separation and morphologies were determined by differential scanning calorimetry. To this end the phase separation of aqueous mixtures of WPC (12 wt% or 20 wt%) and HPMC (2 wt% or 3 wt%) at pH 5 or 6, was arrested at different times before freeze-drying. Confocal microscopy allowed to characterize the morphology of phase separation. Co-dried mixture from quenched phase-separated systems exhibited different numbers of Tgs, according to the degree of phase separation. Two Tgs were observed in the fully phase-separated systems. A single Tg was detected during the first stages of phase separation (i.e. below a 50% of phase separation). It is proposed to ascribe the observed single Tg to the predominance of the extremely large mixed protein/polysaccharide interface present, that would dominate the mobility of the whole system because acting as a network for the entanglement between the protein-rich and the polysaccharide-rich phases. WPC (12 wt%)/HPMC (2 wt%) co-dried mixture at pH 5, with a degree of phase separation above 50%, exhibited three Tgs which were related respectively to the mixed interface, protein-rich phase and polysaccharide-rich phase. The non-phase-separated WPC (6 wt%)/HPMC (1 wt%) co-dried mixture also showed a single Tg with a reasonable agreement to the predicted value by a theoretical model. © 2009 Elsevier B.V. All rights reserved.
Registro:
| Documento: |
Artículo
|
| Título: | Glass transition temperature of protein/polysaccharide co-dried mixtures as affected by the extent and morphology of phase separation |
| Autor: | Jara, F.L.; Pilosof, A.M.R. |
| Filiación: | Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina
|
| Palabras clave: | Confocal scanning light microscopy; Differential scanning calorimetry; Glass transition temperature; Hydroxypropyl methylcellulose; Phase behaviour; Whey protein concentrate; Calorimeters; Confocal microscopy; Curing; Differential scanning calorimetry; Glass; Glass transition; Laser interferometry; Mixtures; Phase interfaces; Phase modulation; Polysaccharides; Scanning; Superconducting transition temperature; Confocal scanning light microscopy; Glass transition temperature; Hydroxypropyl methylcellulose; Phase behaviour; Whey protein concentrate; Phase separation |
| Año: | 2009
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| Volumen: | 487
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| Número: | 1-2
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| Página de inicio: | 65
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| Página de fin: | 73
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| DOI: |
http://dx.doi.org/10.1016/j.tca.2009.01.012 |
| Título revista: | Thermochimica Acta
|
| Título revista abreviado: | Thermochim Acta
|
| ISSN: | 00406031
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| CODEN: | THACA
|
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00406031_v487_n1-2_p65_Jara |
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Citas:
---------- APA ----------
Jara, F.L. & Pilosof, A.M.R.
(2009)
. Glass transition temperature of protein/polysaccharide co-dried mixtures as affected by the extent and morphology of phase separation. Thermochimica Acta, 487(1-2), 65-73.
http://dx.doi.org/10.1016/j.tca.2009.01.012---------- CHICAGO ----------
Jara, F.L., Pilosof, A.M.R.
"Glass transition temperature of protein/polysaccharide co-dried mixtures as affected by the extent and morphology of phase separation"
. Thermochimica Acta 487, no. 1-2
(2009) : 65-73.
http://dx.doi.org/10.1016/j.tca.2009.01.012---------- MLA ----------
Jara, F.L., Pilosof, A.M.R.
"Glass transition temperature of protein/polysaccharide co-dried mixtures as affected by the extent and morphology of phase separation"
. Thermochimica Acta, vol. 487, no. 1-2, 2009, pp. 65-73.
http://dx.doi.org/10.1016/j.tca.2009.01.012---------- VANCOUVER ----------
Jara, F.L., Pilosof, A.M.R. Glass transition temperature of protein/polysaccharide co-dried mixtures as affected by the extent and morphology of phase separation. Thermochim Acta. 2009;487(1-2):65-73.
http://dx.doi.org/10.1016/j.tca.2009.01.012
