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Abstract:

Thermal transitions were analyzed after freezing and thawing of two commercial baker's yeast cells of Saccharomyces cerevisiae having high and low tolerance to sugar. Yeast in stationary phase of growth were exposed to two cycles of freezing (20 h and 4 h) at -20 °C after being exposed to various physical and chemical mild stresses and differential scanning calorimetry (DSC) runs were performed at 10 °C/min from 20 °C to 100 °C. Thermograms showed two peaks that were attributed to low and high protein onset denaturation temperatures: 47-58 °C and 67-77 °C, respectively. Apparent enthalpy changes showed that the sugar tolerant strain had a relatively higher resistance to freezing and thawing than the non-tolerant one. On the other hand, some pre-stress treatments (e.g., temperature, ethanol and NaCl) increased both enthalpy values and survival after freezing and thawing. The analysis of data clearly showed that DSC can be a very useful tool to analyze the behavior of yeast cells after freezing and thawing stress. © 2007 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Exploring differential scanning calorimetry as a tool for evaluating freezing stress sensitivity in Baker's yeasts
Autor:Santagapita, P.R.; Kronberg, F.; Wu, A.; Cerrutti, P.; Buera, M.P.; Galvagno, M.A.
Filiación:Depto. Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Lab. Microbiología Industrial, Facultad de Ingenieria, Universidad de Buenos Aires, Argentina
IIB-CONICET-UNSAM, Buenos Aires, Argentina
Palabras clave:Denaturation enthalpy; Differential scanning calorimetry; Freezing stress sensitivity; Frozen yeast cells; Trehalose; Denaturation; Differential scanning calorimetry; Freezing; Sensitivity analysis; Thawing; Thermography (temperature measurement); Stress sensitivity; Thawing stress; Yeast cells; Yeast
Año:2007
Volumen:465
Número:1-2
Página de inicio:67
Página de fin:72
DOI: http://dx.doi.org/10.1016/j.tca.2007.09.007
Título revista:Thermochimica Acta
Título revista abreviado:Thermochim Acta
ISSN:00406031
CODEN:THACA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00406031_v465_n1-2_p67_Santagapita

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Citas:

---------- APA ----------
Santagapita, P.R., Kronberg, F., Wu, A., Cerrutti, P., Buera, M.P. & Galvagno, M.A. (2007) . Exploring differential scanning calorimetry as a tool for evaluating freezing stress sensitivity in Baker's yeasts. Thermochimica Acta, 465(1-2), 67-72.
http://dx.doi.org/10.1016/j.tca.2007.09.007
---------- CHICAGO ----------
Santagapita, P.R., Kronberg, F., Wu, A., Cerrutti, P., Buera, M.P., Galvagno, M.A. "Exploring differential scanning calorimetry as a tool for evaluating freezing stress sensitivity in Baker's yeasts" . Thermochimica Acta 465, no. 1-2 (2007) : 67-72.
http://dx.doi.org/10.1016/j.tca.2007.09.007
---------- MLA ----------
Santagapita, P.R., Kronberg, F., Wu, A., Cerrutti, P., Buera, M.P., Galvagno, M.A. "Exploring differential scanning calorimetry as a tool for evaluating freezing stress sensitivity in Baker's yeasts" . Thermochimica Acta, vol. 465, no. 1-2, 2007, pp. 67-72.
http://dx.doi.org/10.1016/j.tca.2007.09.007
---------- VANCOUVER ----------
Santagapita, P.R., Kronberg, F., Wu, A., Cerrutti, P., Buera, M.P., Galvagno, M.A. Exploring differential scanning calorimetry as a tool for evaluating freezing stress sensitivity in Baker's yeasts. Thermochim Acta. 2007;465(1-2):67-72.
http://dx.doi.org/10.1016/j.tca.2007.09.007