Artículo

Diéguez, M.J.; Pergolesi, M.F.; Velasquez, S.M.; Ingala, L.; López, M.; Darino, M.; Paux, E.; Feuillet, C.; Sacco, F. "Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS" (2014) Theoretical and Applied Genetics. 127(5):1133-1141
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Key message: Fine mapping permits the precise positioning of genes within chromosomes, prerequisite for positional cloning that will allow its rational use and the study of the underlying molecular action mechanism. Abstract: Three leaf rust resistance genes were identified in the durable leaf rust resistant Argentinean wheat variety Sinvalocho MA: the seedling resistance gene Lr3 on distal 6BL and two adult plant resistance genes, LrSV1 and LrSV2, on chromosomes 2DS and 3BS, respectively. To develop a high-resolution genetic map for LrSV2, 10 markers were genotyped on 343 F2 individuals from a cross between Sinvalocho MA and Gama6. The closest co-dominant markers on both sides of the gene (3 microsatellites and 2 STMs) were analyzed on 965 additional F2s from the same cross. Microsatellite marker cfb5010 cosegregated with LrSV2 whereas flanking markers were found at 1 cM distal and at 0.3 cM proximal to the gene. SSR markers designed from the sequences of cv Chinese Spring BAC clones spanning the LrSV2 genetic interval were tested on the recombinants, allowing the identification of microsatellite swm13 at 0.15 cM distal to LrSV2. This delimited an interval of 0.45 cM around the gene flanked by the SSR markers swm13 and gwm533 at the subtelomeric end of chromosome 3BS. © 2014, Springer-Verlag Berlin Heidelberg.

Registro:

Documento: Artículo
Título:Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS
Autor:Diéguez, M.J.; Pergolesi, M.F.; Velasquez, S.M.; Ingala, L.; López, M.; Darino, M.; Paux, E.; Feuillet, C.; Sacco, F.
Filiación:Instituto de Genética “Ewald A. Favret” CICVyA-INTA CC25 (1712) Castelar, Buenos Aires, Argentina
Laboratorio de Fisiología y Biología Molecular IFIByNE (CONICET) FCEyN-UBA, Intendente Güiraldes 2160, Buenos Aires, C1428EGA, Argentina
INRA UMR Genetics, Diversity and Ecophysiology of Cereals, Domaine de Crouelle, 5 Chemin de Bealieu, Clermont-Ferrand, Cedex 2, 63039, France
Palabras clave:Chromosomes; Cloning; DNA sequences; Genes; Mapping; Plants (botany); Action mechanisms; Adult plant resistances; Leaf rust resistance genes; Microsatellite markers; Positional cloning; Precise positioning; Seedling resistance; Wheat chromosomes; Genetic engineering; genetic marker; Basidiomycetes; chromosome map; disease resistance; genetic marker; genetics; microbiology; plant chromosome; plant disease; plant gene; wheat; Basidiomycota; Chromosome Mapping; Chromosomes, Plant; Disease Resistance; Genes, Plant; Genetic Markers; Plant Diseases; Triticum
Año:2014
Volumen:127
Número:5
Página de inicio:1133
Página de fin:1141
DOI: http://dx.doi.org/10.1007/s00122-014-2285-z
Título revista:Theoretical and Applied Genetics
Título revista abreviado:Theor. Appl. Genet.
ISSN:00405752
CODEN:THAGA
CAS:Genetic Markers
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00405752_v127_n5_p1133_Dieguez

Referencias:

  • Akhunov, D., Goodyear, A.W., Geng, S., Qi, L., Echalier, B., The organization and rate of evolution of wheat genomes are correlated with recombination rates along chromosome arms (2003) Genome Res, 13, pp. 753-763. , PID: 12695326, COI: 1:CAS:528:DC%2BD3sXjs1Oqtbw%3D
  • Benbouza, H., Jacquemin, J.-M., Baudoin, J.-P., Mergeai, G., Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels (2006) Biotechnol Agron Soc Environ, 10 (2), pp. 77-81. , COI: 1:CAS:528:DC%2BD28XotlSqurw%3D
  • Bossolini, E., Krattinger, S., Keller, B., Development of simple sequence repeat markers specific for the Lr34 resistance region of wheat using sequence information from rice and Aegilops tauschii (2006) Theor Appl Genet, 113 (6), pp. 1049-1062. , PID: 16896711, COI: 1:CAS:528:DC%2BD28XhtVSktbbE
  • Breen, J., Li, D., Dunn, D.S., Békés, F., Kong, X., Zhang, J., Jia, J., Appels, R., Wheat beta-expansin (EXPB11) genes: identification of the expressed gene on chromosome 3BS carrying a pollen allergen domain (2010) BMC Plant Biol, 10, pp. 99-109. , PID: 20507562, COI: 1:CAS:528:DC%2BC3cXntVWmt7s%3D
  • Caldwell, R.M., In: Finley K.W, Shepherd K.W(eds) Breeding for general and/or specific plant disease resistance. Proc. 3rd Int. Wheat Genetics Symp Aust Acad Sci, Canberra: Australia (1968) pp 263–272
  • Choulet, F., Wicker, T., Rustenholz, C., Paux, E., Salse, J., Leroy, P., Schlub, S., Feuillet, C., Megabase level sequencing reveals contrasted organization and evolution patterns of the wheat gene and transposable element spaces (2010) Plant Cell, 22, pp. 1686-1701. , PID: 20581307, COI: 1:CAS:528:DC%2BC3cXhtVCjsrbF
  • Cloutier, S., McCallum, B.D., Loutre, C., Banks, T.W., Wicker, T., Feulliet, C., Keller, B., Jordan, M.C., Leaf rust resistance gene Lr1, isolated from bread wheat (Triticum aestivum L.) is a member of the large psr567 gene family (2007) Plant Mol Biol, 65 (1-2), pp. 93-106. , PID: 17611798, COI: 1:CAS:528:DC%2BD2sXovFSqs7w%3D
  • Diéguez, M.J., Altieri, E., Ingala, L.R., Perera, E., Sacco, F., Naranjo, T., Physical and genetic mapping of AFLPs and the leaf rust resistance Lr3 gene on chromosome 6BL of wheat (2006) Theor Appl Genet, 112, pp. 251-257. , PID: 16215730, COI: 1:CAS:528:DC%2BD2MXhtlajsrzL
  • Favret, E.A., Saione, H.A., Franzone, P.M., New approaches in breeding for disease resistance. Cereal breeding and production Symp. Argentina Special Report 718 (1983) Oregon State University
  • Feuillet, C., Travella, S., Stein, N., Albar, L., Nublat, A., Keller, B., Molecular cloning of a new receptor like–kinase gene encoded at the Lr10 disease resistance locus of wheat (2003) Plant J, 11, pp. 45-52
  • Fu, D., Uauy, C., Distelfeld, A., Blechl, A., Epstein, L., Chen, X., Sela, H., Dubcovsky, J., A kinase-start gene confers temperature-dependent resistance to wheat stripe rust (2009) Science, 323, pp. 1357-1360. , PID: 19228999, COI: 1:CAS:528:DC%2BD1MXisFemtb0%3D
  • Fu, J., Liu, H., Li, Y., Yu, H., Li, X., Xiao, J., Wang, S., Manipulating broad-spectrum disease resistance by suppressing pathogen-induced auxin accumulation in rice (2011) Plant Phys, 155, pp. 589-602. , COI: 1:CAS:528:DC%2BC3MXksFagtLc%3D
  • Germán, S., Barcellos, A., Chaves, M., Kohli, M., Campos, P., de Viedma, L., The situation of common wheat rusts in the Southern Cone of America and perspectives for control (2007) Aust J Agric Res, 58 (6), pp. 620-630
  • Gill, K.S., Gill, B.S., Endo, T.R., A chromosome region-specific mapping strategy reveals gene rich telomeric ends in wheat (1993) Chromosoma, 102, pp. 374-381. , COI: 1:CAS:528:DyaK2cXht1Kltbs%3D
  • Hayden, M.J., Kuchel, H., Chalmers, K.J., Sequence tagged microsatellites for the Xgwm533 locus provide new diagnostic markers to select for the presence of stem rust resistance gene Sr2 in bread wheat (Triticum aestivum L.) (2004) Theor Appl Genet, 109, pp. 1641-1647. , PID: 15340687, COI: 1:CAS:528:DC%2BD2cXps1Onsrk%3D
  • Hayden, M.J., Stephenson, P., Logojan, A.M., Khatkar, D., Rogers, C., Elsden, J., Koebner, M.D., Sharpe, P.J., Development and genetic mapping of sequence-tagged microsatellite (STMs) in bread wheat (Triticum aestivum L.) (2006) Theor Appl Genet, 113, pp. 1271-1281. , PID: 16932882, COI: 1:CAS:528:DC%2BD28XhtVOmu7rI
  • Helguera, M., Khan, I.A., Kolmer, J., Lijavetzky, D., Zhong-qi, L., Dubcovsky, J., PCR assays for the Lr37-Yr17-Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines (2003) Crop Sci, 43, pp. 1839-1847. , COI: 1:CAS:528:DC%2BD3sXnvVSktrY%3D
  • Hiebert, C.W., Thomas, J.B., Somer, D.J., McCallum, B.D., Fox, S.L., Microsatellite mapping of adult-plant leaf rust resistance gene Lr22a in wheat (2007) Theor Appl Genet, 115, pp. 877-884. , PID: 17646964, COI: 1:CAS:528:DC%2BD2sXhtVKru7%2FI
  • Huang, L., Brooks, S.A., Li, W., Fellers, J.P., Gill, B.S., Trick, H.N., Gill, B.S., Map-based cloning of leaf rust resistance gene Lr21 from the large and poliploid genome of bread wheat (2003) Genetics, 164, pp. 655-664. , PID: 12807786, COI: 1:CAS:528:DC%2BD3sXlvFaisLg%3D
  • Ingala, L., Saione, H., Helguera, M., Nisi, M., Sacco F (2005) Inheritance of adult plant resistance genes, and associated markers, from a durable resistant cultivar to leaf rust. In: Buck HT, Nisi JE, Salomon N(eds) Proceedings of the seventh international wheat conference. Mar del Plata, Argentina. Wheat production in stressed environments. Develpments in plant breeding. vol 12. Springer Dordrecht, 2007, pp. 59-64
  • Ingala, L., López, M., Darino, M., Pergolesi, M.F., Diéguez, M.J., Sacco, F., Genetic analysis of leaf rust resistance genes and associated markers in the durable resistant wheat cultivar Sinvalocho MA (2012) Theor Appl Genet, 124 (7), pp. 1305-1314. , PID: 22278178, COI: 1:CAS:528:DC%2BC38Xls1Sitrc%3D
  • Johnson, R., Durable resistance: definition of, genetic control, and attainment in plant breeding (1981) Phytopathology, 71, pp. 567-568
  • Kolmer, J.A., Genetics of resistance to wheat leaf rust (1996) Annu Rev Phytopathol, 34, pp. 435-455. , PID: 15012551, COI: 1:CAS:528:DyaK28XlsFOhsLc%3D
  • Kota, R., Spielmeyer, W., McIntosh, R.A., Lagudah, E.S., Fine genetic mapping fails to dissociate durable stem rust resistance gene Sr2 from pseudo-black chaff in common wheat (Triticum aestivum L.) (2006) Theor Appl Genet, 112, pp. 492-499. , PID: 16311724, COI: 1:CAS:528:DC%2BD28Xnt1yjs7Y%3D
  • Krattinger, S., Lagudah, E.S., Spielmeyer, W., Singh, R.P., Huerta-Espino, J., McFadden, H., Bossolini, E., Keller, B., A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat (2009) Science, 323, pp. 1360-1363. , PID: 19229000, COI: 1:CAS:528:DC%2BD1MXisFemtbg%3D
  • Lander, E., Green, P., Abrahamson, J., Barlow, A., Daley, M., Lincoln, S., Newburg, L., MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations (1987) Genomics, 1, pp. 174-181. , PID: 3692487, COI: 1:CAS:528:DyaL1cXhsVCksrk%3D
  • Ling, H.-Q., Zhu, Y., Keller, B., High-resolution mapping of the leaf rust disease resistance gene Lr1 in wheat and characterization of BAC clones from the Lr1 locus (2003) Theor Appl Genet, 106, pp. 875-882. , PID: 12647062, COI: 1:CAS:528:DC%2BD3sXitFCgs7c%3D
  • Liu, S., Anderson, J.A., Targeted molecular mapping of a major wheat QTL for Fusarium head blight resistance using wheat ESTs and synteny with rice (2003) Genome, 46, pp. 817-823. , PID: 14608398, COI: 1:CAS:528:DC%2BD3sXpsFChtLk%3D
  • Liu, S., Zhang, X., Pumphrey, M.O., Snack, R.W., Gill, B.S., Anderson, J.A., Complex microcolinearity among wheat, rice and barley revealed by fine mapping of the genomic region harbouring a major QTL for resistance to fusarium head blight in wheat (2006) Funct Integr Genomics, 6, pp. 83-89. , PID: 16270217, COI: 1:CAS:528:DC%2BD28XhslWgtbc%3D
  • Lukaszewski, A.J., Curtis, C.A., Physical distribution of recombination in B-genome chromosomes of tetraploid wheat (1993) Theor Appl Genet, 86, pp. 121-127. , PID: 24193391, COI: 1:CAS:528:DyaK3sXkvFWntrw%3D
  • Macagno, L.F., Pizarro, J.B., Cordone, G.E., Dirección Nacional Asistente de Planificación. Publicación miscelánea N° 4 (1993) INTA, pp. 15-28
  • Mago, R., Tabe, L., McIntosh, R.A., Pretorius, Z., Kota, R., Paux, E., Wicker, T., Spielmeyer, W., A multiple resistance locus on chromosome arm 3BS in wheat confers resistance to stem rust (Sr2), leaf rust (Lr27) and powdery mildew (2011) Theor Appl Genet, 123 (4), pp. 615-623. , PID: 21573954, COI: 1:CAS:528:DC%2BC3MXpsFCqtLs%3D
  • Mains, E.B., Jackson, H.S., Physiologic specialization in the leaf rust of wheat; Puccinia triticina Erikss (1926) Phytopathology, 16, pp. 89-120
  • McIntosh, R.A., Welllings, C.R., Park, R.F., (1995) Wheat rusts: an atlas of resistance genes, , Kluwer, Dordrecht
  • Messmer, M.M., Seyfarth, R., Keller, M., Schachermayr, G., Winzeler, M., Zanetti, S., Feuillet, C., Keller, B., Genetic analysis of durable leaf rust resistance in winter wheat (2000) Theor Appl Genet, 100, pp. 419-431. , COI: 1:CAS:528:DC%2BD3cXisVaktro%3D
  • Michelmore, R.W., Meyers, B.C., Clusters of resistance genes in plants evolve by divergent selection and a birth-and-death process (1998) Genome Res, 8, pp. 1113-1130. , PID: 9847076, COI: 1:CAS:528:DyaK1cXotVWhurg%3D
  • Michelmore, R.W., Paran, I., Kesseli, V., Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations (1991) Proc Natl Acad Sci USA, 88, pp. 9828-9832. , PID: 1682921, COI: 1:CAS:528:DyaK38Xhs1emtw%3D%3D
  • Paux, E., Roger, D., Badaeva, E., Gay, G., Bernard, M., Sourdille, P., Feuillet, C., Characterizing the composition and evolution of homoeologous genomes in hexaploid wheat through BAC-end sequencing on chromosome 3B (2006) Plant J, 48, pp. 463-474. , PID: 17010109, COI: 1:CAS:528:DC%2BD28Xht1ahsLbN
  • Paux, E., Sourdille, P., Salse, J., Saintenac, C., Choulet, F., Leroy, P., Korol, A., Feuillet, C., A physical map of the 1 gigabase bread wheat chromosome 3B (2008) Science, 322, pp. 101-104. , PID: 18832645, COI: 1:CAS:528:DC%2BD1cXhtFyhurnK
  • Pink, D.A.C., Strategies using genes for non-durable resistance (2002) Euphytica, 1, pp. 227-236
  • Pretorius, Z.A., Roelfs, A.P., The role of Lr10, Lr13, and Lr34 in the expression of adult-plant resistance to leaf rust in the wheat cultivar Era (1996) Plant Dis, 80 (2), pp. 199-202
  • Röder, M., Korzun, V., Wendehake, K., Plaschke, J., Tixier, M.-H., Leroy, P., Ganal, M.W., (1998) A microsatellite map of wheat. genetics, 149, pp. 2007-2023
  • Rodriguez Amieva, P.J., Tessi, J.L., Frecha, J.H., Vallega, J., Estimación de los daños producidos en la Argentina por las royas del tallo y de la hoja del trigo durante el período 1949–1958 (1961) Robigo, 12, pp. 18-22
  • Rozen, S., Skaletsky, H.J., Primer3 on the WWW for general users and for biologist programmers (2000) Bioinformatics methods and protocols: methods in molecular biology, pp. 365-386. , Krawetz S, Misener S, (eds), Humana Press, Totowa
  • Rubiales, D., Niks, R.E., Characterization of Lr34, a major gene conferring nonhypersensitive resistance to wheat leaf rust (1995) Plant Dis, 79, pp. 1208-1212
  • Rustenholz, C., Choulet, F., Laugier, C., Safar, J., Simkova, H., Dolezel, J., Magni, F., Paux, E., A 3000-loci transcription map of chromosome 3b unravels the structural and functional features of gene islands in hexaploid wheat (2011) Plant Physiol, 157, pp. 1596-1608. , PID: 22034626, COI: 1:CAS:528:DC%2BC3MXhs1ekt7fP
  • Sacco, F., Favret, E.A., Suarez, E.Y., Solari, R.M., Saione, H.A., Spontaneous genetic variation for leaf rust reaction in Sinvalocho MA wheat (1995) J Phytopathol (Berl.), 143, pp. 251-255
  • Sacco, F., Suárez, E.Y., Naranjo, T., Mapping of the leaf rust resistance gene Lr3 on chromosome 6B of Sinvalocho MA wheat (1998) Genome, 41, pp. 686-690. , COI: 1:CAS:528:DyaK1cXntl2ht70%3D
  • Safar, J., Bartos, J., Janda, J., Dissecting large and complex genomes: flow sorting and BAC cloning of individual chromosomes from bread wheat (2004) Plant J., 39, pp. 960-968. , PID: 15341637, COI: 1:CAS:528:DC%2BD2cXpt1Gntb0%3D
  • Saintenac, C., Falque, M., Martin, O., Paux, E., Feuillet, C., Sourdille, P., Detailed recombination studies along chromosome 3b provide new insights on crossover distribution in wheat (Triticum aestivum L.) (2009) Genetics, 181, pp. 393-403. , PID: 19064706, COI: 1:CAS:528:DC%2BD1MXktVegsb8%3D
  • Saintenac, C., Faure, S., Remay, A., Choulet, F., Ravel, C., Paux, E., Balfourier, F., Sourdille, P., Variation in crossover rates across a 3-Mb contig of bread wheat (Triticum aestivum) reveals the presence of a meiotic recombination hotspot (2011) Chromosoma, 120, pp. 185-198. , PID: 21161258, COI: 1:CAS:528:DC%2BC3MXjtFGntLw%3D
  • Sawhney, R.N., Nayar, S.K., Sharma, J.B., Bedi, R., Mechanism of durable resistance: a new approach (1989) Theor Appl Genet, 78, pp. 229-232. , PID: 24227149, COI: 1:STN:280:DC%2BC2c7mt1Oguw%3D%3D
  • Schnurbusch, T., Paillard, S., Schori, A., Messmer, M., Schachermayr, G., Winzeler, M., Keller, B., Dissection of quantitative and durable leaf rust resistance in swiss winter wheat reveals a major resistance QTL in the Lr34 chromosomal region (2004) Theor Appl Genet, 108, pp. 477-484. , PID: 14523520, COI: 1:STN:280:DC%2BD2c%2FmtFGhtw%3D%3D
  • Singh, S., Bowden, R., Molecular Mapping of adult-plant race-specific leaf rust resistance gene Lr12 in bread wheat (2011) Mol Breed, 28, pp. 137-142
  • Somers, D.J., Isaac, P., Edwards, K., A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.) (2004) Theor Appl Genet, 109, pp. 1105-1114. , PID: 15490101, COI: 1:CAS:528:DC%2BD2cXotlKqtbs%3D
  • Song, Q.J., Shi, J.R., Singh, S., Fickus, W., Costa, J.M., Lewis, J., Gill, B.S., Cregan, P.B., Development and mapping of microsatellite (SSR) markers in wheat (2005) Theor Appl Genet, 110, pp. 550-560. , PID: 15655666, COI: 1:CAS:528:DC%2BD2MXhsVyms7k%3D
  • Sourdille, P., Singh, S., Cadalen, T., Brown-Guedira, G., Gay, G., Qi, L., Gill, B.S., Bernard, M., Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.) (2004) Funct Integr Genomics, 4 (1), pp. 12-25. , PID: 15004738, COI: 1:CAS:528:DC%2BD2cXhsl2hsbc%3D
  • Stein, N., Feuillet, C., Wicker, T., Schlagenhauf, E., Keller, B., Subgenome chromosome walking in wheat: a 450- kb physical contig in Triticum monococcum L. spans the Lr10 resistance locus in hexaploid wheat (Triticum aestivum L.) (2000) Proc Natl Acad Sci, 97, pp. 13436-13441. , PID: 11078510, COI: 1:CAS:528:DC%2BD3cXosVOjsLY%3D
  • Voorrips, R.E., MapChart: software for the graphical presentation of linkage maps and QTLs (2002) J Hered, 93 (1), pp. 77-78. , PID: 12011185, COI: 1:CAS:528:DC%2BD38XktlOntrw%3D
  • Vos, P., Hogers, R., Bleeker, M., Reijans, M., Van de Lee, T., Hornes, M., Friters, A., Zabeau, M., AFLP: a new technique for DNA fingerprinting (1995) Nucleic Acids Res, 23, pp. 4407-4441. , PID: 7501463, COI: 1:CAS:528:DyaK2MXpslensbo%3D
  • Xue, S., Zhang, Z., Lin, F., A high-density intervarietal map of the wheat genome enriched with markers derived from expressed sequence tags (2008) Theor Appl Genet, 117, pp. 181-189. , PID: 18437345, COI: 1:CAS:528:DC%2BD1cXnslKltb8%3D
  • Yan, L., Loukoianov, A., Tranquilli, G., Helguera, M., Fahima, T., Dubcovsky, J., Positional cloning of the wheat vernalization gene VRN1 (2003) Proc Natl Acad Sci, 100 (10), pp. 6263-6268. , PID: 12730378, COI: 1:CAS:528:DC%2BD3sXjvFOksr4%3D

Citas:

---------- APA ----------
Diéguez, M.J., Pergolesi, M.F., Velasquez, S.M., Ingala, L., López, M., Darino, M., Paux, E.,..., Sacco, F. (2014) . Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS. Theoretical and Applied Genetics, 127(5), 1133-1141.
http://dx.doi.org/10.1007/s00122-014-2285-z
---------- CHICAGO ----------
Diéguez, M.J., Pergolesi, M.F., Velasquez, S.M., Ingala, L., López, M., Darino, M., et al. "Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS" . Theoretical and Applied Genetics 127, no. 5 (2014) : 1133-1141.
http://dx.doi.org/10.1007/s00122-014-2285-z
---------- MLA ----------
Diéguez, M.J., Pergolesi, M.F., Velasquez, S.M., Ingala, L., López, M., Darino, M., et al. "Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS" . Theoretical and Applied Genetics, vol. 127, no. 5, 2014, pp. 1133-1141.
http://dx.doi.org/10.1007/s00122-014-2285-z
---------- VANCOUVER ----------
Diéguez, M.J., Pergolesi, M.F., Velasquez, S.M., Ingala, L., López, M., Darino, M., et al. Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS. Theor. Appl. Genet. 2014;127(5):1133-1141.
http://dx.doi.org/10.1007/s00122-014-2285-z