Artículo

Lia, V.V.; Bracco, M.; Gottlieb, A.M.; Poggio, L.; Confalonieri, V.A. "Complex mutational patterns and size homoplasy at maize microsatellite loci" (2007) Theoretical and Applied Genetics. 115(7):981-991
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Abstract:

Microsatellite markers have become one of the most popular tools for germplasm characterization, population genetics and evolutionary studies. To investigate the mutational mechanisms of maize microsatellites, nucleotide sequence information was obtained for ten loci. In addition, Single-Strand Conformation Polymorphism (SSCP) analysis was conducted to assess the occurrence of size homoplasy. Sequence analysis of 54 alleles revealed a complex pattern of mutation at 8/10 loci, with only 2 loci showing allele variation strictly consistent with stepwise mutations. The overall allelic diversity resulted from changes in the number of repeat units, base substitutions, and indels within repetitive and non-repetitive segments. Thirty-one electromorphs sampled from six maize landraces were considered for SSCP analysis. The number of conformers per electromorph ranged from 1 to 7, with 74.2% of the electromorphs showing more than one conformer. Size homoplasy was apparent within landraces and populations. Variation in the amount of size homoplasy was observed within and between loci, although no differences were detected among populations. The results of the present study provide useful information on the interpretation of genetic data derived from microsatellite markers. Further efforts are still needed to determine the impact of these findings on the estimation of population parameters and on the inference of phylogenetic relationships in maize investigations. © 2007 Springer-Verlag.

Registro:

Documento: Artículo
Título:Complex mutational patterns and size homoplasy at maize microsatellite loci
Autor:Lia, V.V.; Bracco, M.; Gottlieb, A.M.; Poggio, L.; Confalonieri, V.A.
Filiación:Laboratorio de Genética, Departamento de Ecología, Genética Y Evolución, Pabellón II - Ciudad Universitaria, I. Guiraldes y Costanera N. s/n, C1428EHA Cd. Auton. de Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas Y Técnicas, Buenos Aires, Argentina
Palabras clave:Data acquisition; Information analysis; Nucleotides; Parameter estimation; Polymorphism; Complex mutational patterns; Maize microsatellite loci; Single Strand Conformation Polymorphism (SSCP); Size homoplasy; Genetic engineering; microsatellite DNA; allele; article; genetics; maize; molecular genetics; mutation; nucleotide sequence; Alleles; Base Sequence; Microsatellite Repeats; Molecular Sequence Data; Mutation; Zea mays; Zea mays
Año:2007
Volumen:115
Número:7
Página de inicio:981
Página de fin:991
DOI: http://dx.doi.org/10.1007/s00122-007-0625-y
Título revista:Theoretical and Applied Genetics
Título revista abreviado:Theor. Appl. Genet.
ISSN:00405752
CODEN:THAGA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00405752_v115_n7_p981_Lia

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Citas:

---------- APA ----------
Lia, V.V., Bracco, M., Gottlieb, A.M., Poggio, L. & Confalonieri, V.A. (2007) . Complex mutational patterns and size homoplasy at maize microsatellite loci. Theoretical and Applied Genetics, 115(7), 981-991.
http://dx.doi.org/10.1007/s00122-007-0625-y
---------- CHICAGO ----------
Lia, V.V., Bracco, M., Gottlieb, A.M., Poggio, L., Confalonieri, V.A. "Complex mutational patterns and size homoplasy at maize microsatellite loci" . Theoretical and Applied Genetics 115, no. 7 (2007) : 981-991.
http://dx.doi.org/10.1007/s00122-007-0625-y
---------- MLA ----------
Lia, V.V., Bracco, M., Gottlieb, A.M., Poggio, L., Confalonieri, V.A. "Complex mutational patterns and size homoplasy at maize microsatellite loci" . Theoretical and Applied Genetics, vol. 115, no. 7, 2007, pp. 981-991.
http://dx.doi.org/10.1007/s00122-007-0625-y
---------- VANCOUVER ----------
Lia, V.V., Bracco, M., Gottlieb, A.M., Poggio, L., Confalonieri, V.A. Complex mutational patterns and size homoplasy at maize microsatellite loci. Theor. Appl. Genet. 2007;115(7):981-991.
http://dx.doi.org/10.1007/s00122-007-0625-y