Abstract:
Methods for short-term BOD analysis (BODst) based on ferricyanide mediator reduction have succeeded in overcoming some problems associated with the standard BOD test analysis (BOD5) such as long-term incubations (5 days), the need to dilute samples and low reproducibility. Here we present a bioassay where a Klebsiella pneumoniae environmental strain successfully reduces ferricyanide without de-aeration of the samples with linear BOD5 ranges between 30 and 500 mg L -1 or 30 and 200 mg L-1, using glucose-glutamic acid solution (GGA) or OECD standards respectively. We further propose a new assay termination solution that allows higher reproducibility and standardization of the cell-based assay, employing formaldehyde (22.7 g L-1) or other compounds in order to stop ferricyanide reduction without affecting the amperometric detection and therefore replace the centrifugation step normally used to stop microbial-driven reactions in ferricyanide-mediated bioassays. These improvements led to an accurate determination of real municipal wastewater samples. © 2011 Elsevier B.V. All rights reserved.
Registro:
Documento: |
Artículo
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Título: | Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay |
Autor: | Bonetto, M.C.; Sacco, N.J.; Ohlsson, A.H.; Cortón, E. |
Filiación: | Department of Biochemistry, Faculty of Sciences, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires C1428EGA, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET, Argentina Agencia Nacional de Promoción Científica y Tecnológica, ANPCyT, Argentina Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales UBA, Intendente Güiraldes s/n., C1428EGA Ciudad Autónoma de Buenos Aires, Argentina
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Palabras clave: | Amperometric; Bioassay; BODSEED; Ferricyanide; Formaldehyde; Klebsiella pneumoniae; Amino acids; Bacteria; Formaldehyde; Oxygen; Amperometric; Amperometric detection; BODSEED; Environmental strain; Ferricyanide; Klebsiella pneumoniae; Long-term incubation; Municipal wastewaters; Bioassay |
Año: | 2011
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Volumen: | 85
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Número: | 1
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Página de inicio: | 455
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Página de fin: | 462
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DOI: |
http://dx.doi.org/10.1016/j.talanta.2011.04.007 |
Título revista: | Talanta
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Título revista abreviado: | Talanta
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ISSN: | 00399140
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CODEN: | TLNTA
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00399140_v85_n1_p455_Bonetto |
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Citas:
---------- APA ----------
Bonetto, M.C., Sacco, N.J., Ohlsson, A.H. & Cortón, E.
(2011)
. Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay. Talanta, 85(1), 455-462.
http://dx.doi.org/10.1016/j.talanta.2011.04.007---------- CHICAGO ----------
Bonetto, M.C., Sacco, N.J., Ohlsson, A.H., Cortón, E.
"Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay"
. Talanta 85, no. 1
(2011) : 455-462.
http://dx.doi.org/10.1016/j.talanta.2011.04.007---------- MLA ----------
Bonetto, M.C., Sacco, N.J., Ohlsson, A.H., Cortón, E.
"Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay"
. Talanta, vol. 85, no. 1, 2011, pp. 455-462.
http://dx.doi.org/10.1016/j.talanta.2011.04.007---------- VANCOUVER ----------
Bonetto, M.C., Sacco, N.J., Ohlsson, A.H., Cortón, E. Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay. Talanta. 2011;85(1):455-462.
http://dx.doi.org/10.1016/j.talanta.2011.04.007