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Abstract:

Methods for short-term BOD analysis (BODst) based on ferricyanide mediator reduction have succeeded in overcoming some problems associated with the standard BOD test analysis (BOD5) such as long-term incubations (5 days), the need to dilute samples and low reproducibility. Here we present a bioassay where a Klebsiella pneumoniae environmental strain successfully reduces ferricyanide without de-aeration of the samples with linear BOD5 ranges between 30 and 500 mg L -1 or 30 and 200 mg L-1, using glucose-glutamic acid solution (GGA) or OECD standards respectively. We further propose a new assay termination solution that allows higher reproducibility and standardization of the cell-based assay, employing formaldehyde (22.7 g L-1) or other compounds in order to stop ferricyanide reduction without affecting the amperometric detection and therefore replace the centrifugation step normally used to stop microbial-driven reactions in ferricyanide-mediated bioassays. These improvements led to an accurate determination of real municipal wastewater samples. © 2011 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay
Autor:Bonetto, M.C.; Sacco, N.J.; Ohlsson, A.H.; Cortón, E.
Filiación:Department of Biochemistry, Faculty of Sciences, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires C1428EGA, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET, Argentina
Agencia Nacional de Promoción Científica y Tecnológica, ANPCyT, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales UBA, Intendente Güiraldes s/n., C1428EGA Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:Amperometric; Bioassay; BODSEED; Ferricyanide; Formaldehyde; Klebsiella pneumoniae; Amino acids; Bacteria; Formaldehyde; Oxygen; Amperometric; Amperometric detection; BODSEED; Environmental strain; Ferricyanide; Klebsiella pneumoniae; Long-term incubation; Municipal wastewaters; Bioassay
Año:2011
Volumen:85
Número:1
Página de inicio:455
Página de fin:462
DOI: http://dx.doi.org/10.1016/j.talanta.2011.04.007
Título revista:Talanta
Título revista abreviado:Talanta
ISSN:00399140
CODEN:TLNTA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00399140_v85_n1_p455_Bonetto

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Citas:

---------- APA ----------
Bonetto, M.C., Sacco, N.J., Ohlsson, A.H. & Cortón, E. (2011) . Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay. Talanta, 85(1), 455-462.
http://dx.doi.org/10.1016/j.talanta.2011.04.007
---------- CHICAGO ----------
Bonetto, M.C., Sacco, N.J., Ohlsson, A.H., Cortón, E. "Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay" . Talanta 85, no. 1 (2011) : 455-462.
http://dx.doi.org/10.1016/j.talanta.2011.04.007
---------- MLA ----------
Bonetto, M.C., Sacco, N.J., Ohlsson, A.H., Cortón, E. "Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay" . Talanta, vol. 85, no. 1, 2011, pp. 455-462.
http://dx.doi.org/10.1016/j.talanta.2011.04.007
---------- VANCOUVER ----------
Bonetto, M.C., Sacco, N.J., Ohlsson, A.H., Cortón, E. Assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated BOD assay. Talanta. 2011;85(1):455-462.
http://dx.doi.org/10.1016/j.talanta.2011.04.007