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Abstract:

The roles of chemical kinetics and mass transfer in three types of bioreactors (packed-column reactors, rotating disk bioreactors and amperometric detector), used with continuous-flow sample/reagent(s) processing, are discussed in detail. A normalized quantitative comparison between these types of reactors clearly shows that rotating disk reactors afford a significantly more efficient utilization of active sites and permit the effective utilization of very small amounts of biocatalysts. Horseradish peroxidase (EC 1.11.1.7), in presence of hydrogen peroxide catalyses the oxidation of [Os(bpy)2Cl(pyCOOH)]Cl. The electrochemical reduction back of this cosubstrate is detected on glassy carbon electrode surface at 0.00 V. Furthermore, the critical effect of substrate and cosubstrate concentration on amperometric immunosensors construction in which HRP is used as an enzymatic label was studied. © 2004 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Continuous-flow system for horseradish peroxidase enzyme assay comprising a packed-column, an amperometric detector and a rotating bioreactor
Autor:Salinas, E.; Torriero, A.A.J.; Sanz, M.I.; Battaglini, F.; Raba, J.
Filiación:Department of Chemistry, National University of San Luis, Chacabuco 900 y Pedernera, 5700 San Luis, Argentina
INQUIMAE-University of Buenos Aires, Ciudad Universitaria, Pabellón 2, 1428 Buenos Aires, Argentina
Palabras clave:Flow system; Horseradish peroxidase; Osmium; Packed-column reactors; Rotating disk bioreactors; Bioassay; Biocatalysts; Bioreactors; Escherichia coli; Hydrogen peroxide; Immunosensors; Osmium; Oxidation; Polymerization; Cell growth; Flow system; Horseradish peroxidase; Packed-column reactors; Enzymes
Año:2005
Volumen:66
Número:1
Página de inicio:92
Página de fin:102
DOI: http://dx.doi.org/10.1016/j.talanta.2004.10.002
Título revista:Talanta
Título revista abreviado:Talanta
ISSN:00399140
CODEN:TLNTA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00399140_v66_n1_p92_Salinas

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Citas:

---------- APA ----------
Salinas, E., Torriero, A.A.J., Sanz, M.I., Battaglini, F. & Raba, J. (2005) . Continuous-flow system for horseradish peroxidase enzyme assay comprising a packed-column, an amperometric detector and a rotating bioreactor. Talanta, 66(1), 92-102.
http://dx.doi.org/10.1016/j.talanta.2004.10.002
---------- CHICAGO ----------
Salinas, E., Torriero, A.A.J., Sanz, M.I., Battaglini, F., Raba, J. "Continuous-flow system for horseradish peroxidase enzyme assay comprising a packed-column, an amperometric detector and a rotating bioreactor" . Talanta 66, no. 1 (2005) : 92-102.
http://dx.doi.org/10.1016/j.talanta.2004.10.002
---------- MLA ----------
Salinas, E., Torriero, A.A.J., Sanz, M.I., Battaglini, F., Raba, J. "Continuous-flow system for horseradish peroxidase enzyme assay comprising a packed-column, an amperometric detector and a rotating bioreactor" . Talanta, vol. 66, no. 1, 2005, pp. 92-102.
http://dx.doi.org/10.1016/j.talanta.2004.10.002
---------- VANCOUVER ----------
Salinas, E., Torriero, A.A.J., Sanz, M.I., Battaglini, F., Raba, J. Continuous-flow system for horseradish peroxidase enzyme assay comprising a packed-column, an amperometric detector and a rotating bioreactor. Talanta. 2005;66(1):92-102.
http://dx.doi.org/10.1016/j.talanta.2004.10.002