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11β-hydroxyprogesterone (HOP) and 11-ketoprogesterone (KP) are reversible components of a shuttle pair whose interconversion in rat liver is catalyzed by isoform-1 of 11β-hydroxysteroid dehydrogenase. Kidneys also produce this interconversion. The present study was carried out to investigate the shuttle pair and its components in the rat. As in corticosterone/11 -dehydrocorticosterone, oxidation is more effective at an alkaline pH, while reduction prevails at a neutral pH. Moreover, both reactions are inhibited by the detergent 3-[(3-cholamido propyl)- dimethylammonio]-1-propane-sulphonate (CHAPS). However, at variance with the 11-ketosteroids cortisone (E) and 11-dehydrocorticosterone (A) thought to be 'inactive,' KP has slight direct Na+-retaining properties, and it, as well as HOP, induces glucocorticoids (11β-hydroxycorticoids) to retain sodium. 11-ketoprogesterone exhibits 17 times better affinity for native type I mineralocorticoid receptor than HOP and a 3-fold affinity for partially purified (transcortin free) mineralocorticoid receptor. However, KP, in contrast to HOP, binds only weakly to transcortin, not at all to glucocorticoid receptor, and requires reduction at C11 for tyrosine aminotransferase (TAT) induction.


Documento: Artículo
Título:Features of the shuttle pair 11β-hydroxyprogesterone-11- ketoprogesterone
Autor:Galigniana, M.D.; Vicent, G.P.; Burton, G.; Lantos, C.P.
Filiación:Laboratorio de Esteroides, Depto. de Quim. Biológica, Universidad de Buenos Aires, Buenos Aires, Argentina
Depto. de Quim. Orgánica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Laboratorio de Esteroides, Depto. de Quimica Biológica, Universidad de Buenos Aires, (1428) Buenos Aires, Argentina
Palabras clave:11β-hydroxyprogesterone; 11β-hydroxysteroid dehydrogenase; 11-ketoprogesterone; shuttle pair; sodium retention; 11beta hydroxyprogesterone; 11beta hydroxysteroid dehydrogenase; 3 [(3 cholamidopropyl)dimethylammonio] 1 propanesulfonic acid; corticosterone; dehydrocorticosterone; mineralocorticoid receptor; animal cell; animal experiment; animal model; animal tissue; article; enzyme activity; male; nonhuman; oxidation; ph; rat; receptor binding; shuttle vector; sodium retention; tissue specificity; 11-beta-Hydroxysteroid Dehydrogenases; Adrenalectomy; Animals; Binding, Competitive; Cholic Acids; Enzyme Induction; Hydrogen-Ion Concentration; Hydroxyprogesterones; Hydroxysteroid Dehydrogenases; Male; Progesterone; Rats; Rats, Sprague-Dawley; Receptors, Glucocorticoid; Receptors, Mineralocorticoid; Sodium; Transcortin; Tyrosine Transaminase; Animalia; Humulus
Página de inicio:358
Página de fin:364
Título revista:Steroids
Título revista abreviado:STEROIDS
CAS:11-beta-Hydroxysteroid Dehydrogenases, EC; 11-hydroxyprogesterone, 312-90-3; 11-ketoprogesterone, 516-15-4; 3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate, 75621-03-3; Cholic Acids; Hydroxyprogesterones; Hydroxysteroid Dehydrogenases, EC 1.1.-; Progesterone, 57-83-0; Receptors, Glucocorticoid; Receptors, Mineralocorticoid; Sodium, 7440-23-5; Transcortin, 9010-38-2; Tyrosine Transaminase, EC


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---------- APA ----------
Galigniana, M.D., Vicent, G.P., Burton, G. & Lantos, C.P. (1997) . Features of the shuttle pair 11β-hydroxyprogesterone-11- ketoprogesterone. Steroids, 62(4), 358-364.
---------- CHICAGO ----------
Galigniana, M.D., Vicent, G.P., Burton, G., Lantos, C.P. "Features of the shuttle pair 11β-hydroxyprogesterone-11- ketoprogesterone" . Steroids 62, no. 4 (1997) : 358-364.
---------- MLA ----------
Galigniana, M.D., Vicent, G.P., Burton, G., Lantos, C.P. "Features of the shuttle pair 11β-hydroxyprogesterone-11- ketoprogesterone" . Steroids, vol. 62, no. 4, 1997, pp. 358-364.
---------- VANCOUVER ----------
Galigniana, M.D., Vicent, G.P., Burton, G., Lantos, C.P. Features of the shuttle pair 11β-hydroxyprogesterone-11- ketoprogesterone. STEROIDS. 1997;62(4):358-364.