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Abstract:

In this paper, we studied the effect of heme availability on corticosterone and aldosterone synthesis in rat adrenal. We found that hemin stimulated corticosterone and aldosterone production in adrenal homogenates in a dose-dependent fashion. Hemin administration to rats also provoked an increase in both corticosterone and aldosterone content in adrenal. 3.5- Diethoxycarbonyl-1.4-dihydro-2,4,6-trimethylpyridine (DDC), an inhibitor of liver ferrochelatase activity, was able to inhibit this enzyme in rat adrenal. This resulted in an impairment of heme concentration and consequently adrenal ALA-synthase and porphyrin content were increased. Thus, it was proven that DDC inhibits heme biosynthesis in adrenal as it does in liver. In vivo experiments with rats showed that DDC was able to partially blocked ACTH-mediated corticosterone and aldosterone production while heroin administration was able to partially restore it. These data indicate that heme availability affects steroid biosynthesis in rat adrenal.

Registro:

Documento: Artículo
Título:Heme availability affects corticosterone and aldosterone biosynthesis in rat adrenal
Autor:Martini, C.N.; De Avalos, S.G.V.; Romero, D.G.; De Viale, L.S.M.; Vila, M.D.C.
Filiación:Depto. de Quim. Biológica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Depto. Quim. Biológica, Ciudad Universitaria, Pabellón 2, Piso 4, Cap. Fed. 1428, Argentina
Palabras clave:3.5-diethoxycarbonyl-1,4- dihydro-2,4,6-trimethylpyridine (DDC); Aldosterone; Biosynthesis; Corticosterone; Heme; Rat adrenal; 1,4 dihydro 2,4,6 trimethyl 3,5 pyridinedicarboxylic acid diethyl ester; aldosterone; catalase; corticosterone; heme; hemin; porphyrin; adrenal gland; animal tissue; article; controlled study; heme synthesis; hormonal regulation; hormone synthesis; liver; male; nonhuman; rat; 5-Aminolevulinate Synthetase; Adrenal Glands; Adrenocorticotropic Hormone; Aldosterone; Animals; Corticosterone; Desoxycorticosterone; Dicarbethoxydihydrocollidine; Ferrochelatase; Heme; Hemin; Liver; Male; Porphyrins; Rats; Rats, Sprague-Dawley
Año:1997
Volumen:62
Número:12
Página de inicio:767
Página de fin:770
DOI: http://dx.doi.org/10.1016/S0039-128X(97)00074-3
Título revista:Steroids
Título revista abreviado:Steroids
ISSN:0039128X
CODEN:STEDA
CAS:5-Aminolevulinate Synthetase, EC 2.3.1.37; Adrenocorticotropic Hormone, 9002-60-2; Aldosterone, 52-39-1; Corticosterone, 50-22-6; Desoxycorticosterone, 64-85-7; Dicarbethoxydihydrocollidine, 632-93-9; Ferrochelatase, EC 4.99.1.1; Heme, 14875-96-8; Hemin, 16009-13-5; Porphyrins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0039128X_v62_n12_p767_Martini

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Citas:

---------- APA ----------
Martini, C.N., De Avalos, S.G.V., Romero, D.G., De Viale, L.S.M. & Vila, M.D.C. (1997) . Heme availability affects corticosterone and aldosterone biosynthesis in rat adrenal. Steroids, 62(12), 767-770.
http://dx.doi.org/10.1016/S0039-128X(97)00074-3
---------- CHICAGO ----------
Martini, C.N., De Avalos, S.G.V., Romero, D.G., De Viale, L.S.M., Vila, M.D.C. "Heme availability affects corticosterone and aldosterone biosynthesis in rat adrenal" . Steroids 62, no. 12 (1997) : 767-770.
http://dx.doi.org/10.1016/S0039-128X(97)00074-3
---------- MLA ----------
Martini, C.N., De Avalos, S.G.V., Romero, D.G., De Viale, L.S.M., Vila, M.D.C. "Heme availability affects corticosterone and aldosterone biosynthesis in rat adrenal" . Steroids, vol. 62, no. 12, 1997, pp. 767-770.
http://dx.doi.org/10.1016/S0039-128X(97)00074-3
---------- VANCOUVER ----------
Martini, C.N., De Avalos, S.G.V., Romero, D.G., De Viale, L.S.M., Vila, M.D.C. Heme availability affects corticosterone and aldosterone biosynthesis in rat adrenal. Steroids. 1997;62(12):767-770.
http://dx.doi.org/10.1016/S0039-128X(97)00074-3