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Abstract:

Sodium (1- 14 C) acetate and (5- 3 H) mevalonate were incubated with Bufo vienarum toad parotoid gland and liver tissues. Both labelled compounds were incorporated into cholesterol produced by liver while the incubations with parotoid gland produced no labelled cholesterol. Low and high density lipoproteins isolated from toad plasma were iodinated and used for binding studies. Membrane preparations of paratoid gland showed high affinity binding sites for 125 I-LDL and 125 I-HDL. In addition, while colchicine inhibits the in vitro uptake of ( 3 H) cholesteryl linoleate-LDL into parotoid gland tissue an opposite effect was seen with ( 3 H) cholesteryl linoleate-HDL. The above mentioned results would support the hypothesis that the cholesterol used by the parotoid gland for the biosynthesis of bufalienolides would be produced in the liver, transported by the circulating lipoproteins and incorporated by the glands by a receptor-mediated mechanism. © 1984.

Registro:

Documento: Artículo
Título:Biosynthesis of bufadienolides in toads. V. the origin of the cholesterol used by toad parotoid glands for biosynthesis of bufadienolides
Autor:Santa Coloma, ?.A.; Garraffo, H.M.; Pignataro, O.P.; Charreau, E.H.; Gros, E.G.
Filiación:Instituto de Biología y Medicina Experimental, Obligado 2490, 1428 Buenos Aires, Argentina
Departamento de Química Orgánica, UMYMFOR, Facultad de Ciencias Exactas y Naturales, Pab. 2, 1428 Buenos Aires, Argentina
Palabras clave:bufadienolide; cholesterol; animal cell; mouth; nonhuman; parotid gland; priority journal; Animals; Binding Sites; Biological Transport, Active; Bufanolides; Bufo arenarum; Cholesterol; Cholesterol Esters; Female; Lipoproteins, HDL; Lipoproteins, LDL; Liver; Parotid Gland; Animalia; Anura; Bufo arenarum
Año:1984
Volumen:44
Número:1
Página de inicio:11
Página de fin:22
DOI: http://dx.doi.org/10.1016/S0039-128X(84)80012-4
Título revista:Steroids
Título revista abreviado:Steroids
ISSN:0039128X
CODEN:STEDA
CAS:bufadienolide, 29565-35-3; cholesterol, 57-88-5; Bufanolides; Cholesterol, 57-88-5; Cholesterol Esters; Lipoproteins, HDL; Lipoproteins, LDL; cholesteryl linoleate, 604-33-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0039128X_v44_n1_p11_SantaColoma

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Citas:

---------- APA ----------
Santa Coloma, ?.A., Garraffo, H.M., Pignataro, O.P., Charreau, E.H. & Gros, E.G. (1984) . Biosynthesis of bufadienolides in toads. V. the origin of the cholesterol used by toad parotoid glands for biosynthesis of bufadienolides. Steroids, 44(1), 11-22.
http://dx.doi.org/10.1016/S0039-128X(84)80012-4
---------- CHICAGO ----------
Santa Coloma, ?.A., Garraffo, H.M., Pignataro, O.P., Charreau, E.H., Gros, E.G. "Biosynthesis of bufadienolides in toads. V. the origin of the cholesterol used by toad parotoid glands for biosynthesis of bufadienolides" . Steroids 44, no. 1 (1984) : 11-22.
http://dx.doi.org/10.1016/S0039-128X(84)80012-4
---------- MLA ----------
Santa Coloma, ?.A., Garraffo, H.M., Pignataro, O.P., Charreau, E.H., Gros, E.G. "Biosynthesis of bufadienolides in toads. V. the origin of the cholesterol used by toad parotoid glands for biosynthesis of bufadienolides" . Steroids, vol. 44, no. 1, 1984, pp. 11-22.
http://dx.doi.org/10.1016/S0039-128X(84)80012-4
---------- VANCOUVER ----------
Santa Coloma, ?.A., Garraffo, H.M., Pignataro, O.P., Charreau, E.H., Gros, E.G. Biosynthesis of bufadienolides in toads. V. the origin of the cholesterol used by toad parotoid glands for biosynthesis of bufadienolides. Steroids. 1984;44(1):11-22.
http://dx.doi.org/10.1016/S0039-128X(84)80012-4