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Abstract:

Abstract Recent studies have shown that low concentrations of brassinolide induce a rapid generation of nitric oxide in mesophyll cells of maize leaves, which can be easily detected by fluorimetric methods. In this work we describe a series of natural and synthetic brassinosteroids that are able to trigger in vitro NO production in tomato cells that exhibits dose-response behavior. We propose that this effect can be used to develop a new rapid and very sensitive bioassay for brassinosteroid activity that offers several advantages when compared to the current methodologies. © 2015 Elsevier Inc.

Registro:

Documento: Artículo
Título:A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production
Autor:Tossi, V.E.; Acebedo, S.L.; Cassia, R.O.; Lamattina, L.; Galagovsky, L.R.; Ramírez, J.A.
Filiación:Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, CC1245 (7600), Argentina
UMYMFOR - Departamento de Química Orgánica y CONICET, Universidad de Buenos Aires, Ciudad UniversitariaCiudad de Buenos Aires C1428EGA, Argentina
Palabras clave:Bioactivity assay; Brassinosteroids; Nitric oxide; brassinosteroid; nitric oxide; brassinolide; brassinosteroid; heterocyclic steroid; nitric oxide; analytic method; Article; bioassay; controlled study; fluorometry; in vitro study; intermethod comparison; maize; mesophyll cell; nonhuman; plant leaf; tomato; bioassay; cytology; fluorometry; metabolism; plant cell; procedures; Lycopersicon esculentum; Zea mays; Biological Assay; Brassinosteroids; Fluorometry; Nitric Oxide; Plant Cells; Plant Leaves; Steroids, Heterocyclic; Zea mays
Año:2015
Volumen:102
Página de inicio:46
Página de fin:52
DOI: http://dx.doi.org/10.1016/j.steroids.2015.07.003
Título revista:Steroids
Título revista abreviado:Steroids
ISSN:0039128X
CODEN:STEDA
CAS:nitric oxide, 10102-43-9; brassinolide, 72962-43-7; brassinolide; Brassinosteroids; Nitric Oxide; Steroids, Heterocyclic
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0039128X_v102_n_p46_Tossi

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Citas:

---------- APA ----------
Tossi, V.E., Acebedo, S.L., Cassia, R.O., Lamattina, L., Galagovsky, L.R. & Ramírez, J.A. (2015) . A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production. Steroids, 102, 46-52.
http://dx.doi.org/10.1016/j.steroids.2015.07.003
---------- CHICAGO ----------
Tossi, V.E., Acebedo, S.L., Cassia, R.O., Lamattina, L., Galagovsky, L.R., Ramírez, J.A. "A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production" . Steroids 102 (2015) : 46-52.
http://dx.doi.org/10.1016/j.steroids.2015.07.003
---------- MLA ----------
Tossi, V.E., Acebedo, S.L., Cassia, R.O., Lamattina, L., Galagovsky, L.R., Ramírez, J.A. "A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production" . Steroids, vol. 102, 2015, pp. 46-52.
http://dx.doi.org/10.1016/j.steroids.2015.07.003
---------- VANCOUVER ----------
Tossi, V.E., Acebedo, S.L., Cassia, R.O., Lamattina, L., Galagovsky, L.R., Ramírez, J.A. A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production. Steroids. 2015;102:46-52.
http://dx.doi.org/10.1016/j.steroids.2015.07.003