Artículo

Silvani, V.A.; Fracchia, S.; Fernández, L.; Pérgola, M.; Godeas, A. "A simple method to obtain endophytic microorganisms from field-collected roots" (2008) Soil Biology and Biochemistry. 40(5):1259-1263
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Abstract:

We describe a simple technique for obtaining endophytic microorganisms from field-collected roots. Roots of plant species were surfaced-sterilized, cut into pieces, and then each root piece was transferred to drops of Gel-Gro medium. The number of segments with bacteria and fungi was quantified and those with bacteria and dark septate endophytic (DSE) fungi were plated onto tryptic soy agar (TSA) and malt extract agar (MEA), respectively. When arbuscular mycorrhizal fungi (AMF) grew from root segments, they were inoculated into Ri T-DNA-transformed carrot root cultures. Eight different species of bacteria and five dark septate endophytes were isolated. Thirty AMF isolates were identified and continuous pure cultures established. This easy and inexpensive approach allowed us to culture various endophytic microorganisms in an in vitro system saving time and space. © 2007 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:A simple method to obtain endophytic microorganisms from field-collected roots
Autor:Silvani, V.A.; Fracchia, S.; Fernández, L.; Pérgola, M.; Godeas, A.
Filiación:Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 4to piso Pabellon 2, 1428 Buenos Aires, Argentina
Palabras clave:AMF; Bacteria; DSE; Field roots; In vitro culture; Root endophytes; Bacteria; DNA sequences; Fungi; Field roots; In vitro culture; Root endophytes; Plant cell culture; agar; arbuscular mycorrhiza; bacterium; endophyte; fungus; methodology; quantitative analysis; rhizosphere; root; root vegetable; Arbuscular; Bacteria (microorganisms); Daucus carota; Fungi
Año:2008
Volumen:40
Número:5
Página de inicio:1259
Página de fin:1263
DOI: http://dx.doi.org/10.1016/j.soilbio.2007.11.022
Título revista:Soil Biology and Biochemistry
Título revista abreviado:Soil Biol. Biochem.
ISSN:00380717
CODEN:SBIOA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00380717_v40_n5_p1259_Silvani

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Citas:

---------- APA ----------
Silvani, V.A., Fracchia, S., Fernández, L., Pérgola, M. & Godeas, A. (2008) . A simple method to obtain endophytic microorganisms from field-collected roots. Soil Biology and Biochemistry, 40(5), 1259-1263.
http://dx.doi.org/10.1016/j.soilbio.2007.11.022
---------- CHICAGO ----------
Silvani, V.A., Fracchia, S., Fernández, L., Pérgola, M., Godeas, A. "A simple method to obtain endophytic microorganisms from field-collected roots" . Soil Biology and Biochemistry 40, no. 5 (2008) : 1259-1263.
http://dx.doi.org/10.1016/j.soilbio.2007.11.022
---------- MLA ----------
Silvani, V.A., Fracchia, S., Fernández, L., Pérgola, M., Godeas, A. "A simple method to obtain endophytic microorganisms from field-collected roots" . Soil Biology and Biochemistry, vol. 40, no. 5, 2008, pp. 1259-1263.
http://dx.doi.org/10.1016/j.soilbio.2007.11.022
---------- VANCOUVER ----------
Silvani, V.A., Fracchia, S., Fernández, L., Pérgola, M., Godeas, A. A simple method to obtain endophytic microorganisms from field-collected roots. Soil Biol. Biochem. 2008;40(5):1259-1263.
http://dx.doi.org/10.1016/j.soilbio.2007.11.022