Abstract:
We studied controls on nitrification in an undisturbed water-limited ecosystem by inhibiting autotrophic nitrifying bacteria in soils with varying levels of vegetative cover. The activity of nitrifying bacteria was disrupted using nitrapyrin, 2-chloro-6-(trichloromethyl)-pyridine, under field conditions in three microenvironments (underneath shrubs, next to grasses and in bare soil). Ammonia-oxidising bacteria were detected by PCR analysis of DNA in soils. The inhibition of nitrification changed the concentrations of NO 3 - and NH4 + in the soil, while the microenvironment was most important in determining the response of bacteria to the inhibitor. Nitrapyrin application resulted in a significant (p < 0.05) reduction in soil NO3 - concentration (39%) and a significant increase (p < 0.001) in soil NH4 + concentration (41%). Untreated bare-soil microenvironments had the lowest concentrations of NH4 + (1.57 μg/g of dry soil) and NO3 - (0.49 μg/g of dry soil) when compared to the other microenvironments, and showed the highest impacts of nitrification inhibition. For example, NH4 + concentrations increased 288% and NO3 - concentrations decreased 60% in inhibited bare-soil microenvironments. In contrast, untreated microenvironments underneath shrubs had the highest levels of NH4 + (10.01 μg/g of dry soil) and NO3 - (0.69 μg/g of dry soil), but showed no significant effects of inhibition of nitrification on soil nitrogen concentrations. © 2003 Elsevier Ltd. All rights reserved.
Registro:
Documento: |
Artículo
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Título: | Controls on nitrification in a water-limited ecosystem: Experimental inhibition of ammonia-oxidising bacteria in the Patagonian steppe |
Autor: | López, N.I.; Austin, A.T.; Sala, O.E.; Méndez, B.S. |
Filiación: | Depto. de Quím. Biol., Fac. de Ciencias Exactas y Naturales, Cd. Universitaria Pabellón 2, 1428 Buenos Aires, Argentina Catedra de Ecologia - IFEVA, Facultad de Agronomía, CONICET, Buenos Aires, Argentina
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Palabras clave: | Ammonia monooxygenase gene (amoA); Ammonia-oxidising bacteria; Nitrapyrin; Nitrification; Nitrogen; Semi-arid ecosystems; Bacteria; DNA; Ecosystems; Nitrification; Nitrogen compounds; Microenvironment; Soil pollution; ammonia; nitrification; nitrifying bacterium; semiarid region; steppe; Argentina; Patagonia; Bacteria (microorganisms); Poaceae |
Año: | 2003
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Volumen: | 35
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Número: | 12
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Página de inicio: | 1609
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Página de fin: | 1613
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DOI: |
http://dx.doi.org/10.1016/j.soilbio.2003.08.005 |
Título revista: | Soil Biology and Biochemistry
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Título revista abreviado: | Soil Biol. Biochem.
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ISSN: | 00380717
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CODEN: | SBIOA
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00380717_v35_n12_p1609_Lopez |
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Citas:
---------- APA ----------
López, N.I., Austin, A.T., Sala, O.E. & Méndez, B.S.
(2003)
. Controls on nitrification in a water-limited ecosystem: Experimental inhibition of ammonia-oxidising bacteria in the Patagonian steppe. Soil Biology and Biochemistry, 35(12), 1609-1613.
http://dx.doi.org/10.1016/j.soilbio.2003.08.005---------- CHICAGO ----------
López, N.I., Austin, A.T., Sala, O.E., Méndez, B.S.
"Controls on nitrification in a water-limited ecosystem: Experimental inhibition of ammonia-oxidising bacteria in the Patagonian steppe"
. Soil Biology and Biochemistry 35, no. 12
(2003) : 1609-1613.
http://dx.doi.org/10.1016/j.soilbio.2003.08.005---------- MLA ----------
López, N.I., Austin, A.T., Sala, O.E., Méndez, B.S.
"Controls on nitrification in a water-limited ecosystem: Experimental inhibition of ammonia-oxidising bacteria in the Patagonian steppe"
. Soil Biology and Biochemistry, vol. 35, no. 12, 2003, pp. 1609-1613.
http://dx.doi.org/10.1016/j.soilbio.2003.08.005---------- VANCOUVER ----------
López, N.I., Austin, A.T., Sala, O.E., Méndez, B.S. Controls on nitrification in a water-limited ecosystem: Experimental inhibition of ammonia-oxidising bacteria in the Patagonian steppe. Soil Biol. Biochem. 2003;35(12):1609-1613.
http://dx.doi.org/10.1016/j.soilbio.2003.08.005