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Abstract:

We studied controls on nitrification in an undisturbed water-limited ecosystem by inhibiting autotrophic nitrifying bacteria in soils with varying levels of vegetative cover. The activity of nitrifying bacteria was disrupted using nitrapyrin, 2-chloro-6-(trichloromethyl)-pyridine, under field conditions in three microenvironments (underneath shrubs, next to grasses and in bare soil). Ammonia-oxidising bacteria were detected by PCR analysis of DNA in soils. The inhibition of nitrification changed the concentrations of NO 3 - and NH4 + in the soil, while the microenvironment was most important in determining the response of bacteria to the inhibitor. Nitrapyrin application resulted in a significant (p < 0.05) reduction in soil NO3 - concentration (39%) and a significant increase (p < 0.001) in soil NH4 + concentration (41%). Untreated bare-soil microenvironments had the lowest concentrations of NH4 + (1.57 μg/g of dry soil) and NO3 - (0.49 μg/g of dry soil) when compared to the other microenvironments, and showed the highest impacts of nitrification inhibition. For example, NH4 + concentrations increased 288% and NO3 - concentrations decreased 60% in inhibited bare-soil microenvironments. In contrast, untreated microenvironments underneath shrubs had the highest levels of NH4 + (10.01 μg/g of dry soil) and NO3 - (0.69 μg/g of dry soil), but showed no significant effects of inhibition of nitrification on soil nitrogen concentrations. © 2003 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Controls on nitrification in a water-limited ecosystem: Experimental inhibition of ammonia-oxidising bacteria in the Patagonian steppe
Autor:López, N.I.; Austin, A.T.; Sala, O.E.; Méndez, B.S.
Filiación:Depto. de Quím. Biol., Fac. de Ciencias Exactas y Naturales, Cd. Universitaria Pabellón 2, 1428 Buenos Aires, Argentina
Catedra de Ecologia - IFEVA, Facultad de Agronomía, CONICET, Buenos Aires, Argentina
Palabras clave:Ammonia monooxygenase gene (amoA); Ammonia-oxidising bacteria; Nitrapyrin; Nitrification; Nitrogen; Semi-arid ecosystems; Bacteria; DNA; Ecosystems; Nitrification; Nitrogen compounds; Microenvironment; Soil pollution; ammonia; nitrification; nitrifying bacterium; semiarid region; steppe; Argentina; Patagonia; Bacteria (microorganisms); Poaceae
Año:2003
Volumen:35
Número:12
Página de inicio:1609
Página de fin:1613
DOI: http://dx.doi.org/10.1016/j.soilbio.2003.08.005
Título revista:Soil Biology and Biochemistry
Título revista abreviado:Soil Biol. Biochem.
ISSN:00380717
CODEN:SBIOA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00380717_v35_n12_p1609_Lopez

Referencias:

  • Bauhus, J., Meyer, A.C., Brumme, R., Effects of the inhibitors nitrapyrin and sodium chlorate on nitrification and N2O formation in an acid forest soil (1996) Biology and Fertility of Soils, 22, pp. 318-325
  • Charley, J., West, N., Plant-induced soil chemical patterns in some shrub-dominated semi-desert ecosystems in Utah (1975) Journal of Ecology, 63, pp. 945-963
  • Fernández, R.J., Sala, O.E., Golluscio, R.A., Woody and herbaceous aboveground production of a Patagonian steppe (1991) Journal of Range Management, 44, pp. 434-437
  • Gerards, S., Duyts, H., Laanbroek, H.J., Ammonium induced inhibition of ammonium-starved Nitrosomonas europaea cells in soil and sand slurries (1998) FEMS Microbiology Ecology, 26, pp. 269-280
  • Golluscio, R.A., Sala, O.E., Plant functional types and ecological strategies in Patagonian forbs (1993) Journal of Vegetation Science, 4, pp. 839-846
  • Goring, C.A.I., Control of nitrification by 2-chloro-6-(trichloromethyl) pyridine (1962) Soil Science, 93, pp. 211-218
  • Iizumi, T., Misumoto, M., Nakamura, K., A bioluminiscence assay using Nitrosomonas euopaea for rapid and sensitive detection of nitrification inhibitors (1998) Applied and Environmental Microbiology, 64, pp. 3656-3662
  • Jobbágy, E.G., Sala, O.E., Controls on grass and shrub aboveground production in the Patagonian steppe (2000) Ecological Applications, 10, pp. 541-549
  • López, N.I., Méndez, B.S., (2000) Uncultured Ammonium-oxidizing Bacterium UBA6-1 Partial amoA Gene for Ammonia Monooxygenase EMBL Nucleotide Sequence Database, , Accession #AJ277459
  • McTavish, H., Fuchs, J.A., Hooper, A.B., Sequence of the gene coding for ammonia monooxygenase in Nitrosomonas europaea (1993) Journal of Bacteriology, 175, pp. 2436-2444
  • Norton, J.M., Low, J.M., Klotz, M.G., The gene encoding ammonia monooxygenase subunit A exists in three nearly identical copies in Nitrosospira sp. NpA V (1996) FEMS Microbiology Letters, 139, pp. 181-188
  • Paul, E.A., Clark, F.E., (1996) Soil Microbiology and Biochemistry, , Academic Press, San Diego, CA
  • Powell, J., Prosser, J.I., Protection of Nitrosomonas europaea colonizing clay minerals from inhibition by nitrapyrin (1991) Journal of General Microbiology, 137, pp. 1923-1923
  • Robertson, G.P., Wedin, D., Groffman, P.M., Blair, J.M., Holland, E.A., Nadelhoffer, K.J., Harris, D., Soil carbon and nitrogen availability: Nitrogen mineralization, nitrification, and soil respiration potentials (1999) Standard Soil Methods in Long-Term Ecological Research, pp. 258-271. , Robertson, G.P., Coleman, D.C., Bledsoe, C.S., Sollins, P. (Eds.), Oxford University Press, Oxford
  • Robertson, G.P., Sollins, P., Ellis, B.G., Lajtha, K., Exchangeable ions, pH and cation exchange capacity (1999) Standard Soil Methods in Long-Term Ecological Research, pp. 106-114. , Robertson, G.P., Coleman, D.C., Bledsoe, C.S., Sollins, P. (Eds.), Oxford University Press, Oxford
  • Rotthauwe, J.H., Witzel, K.P., Liesack, W., The ammonia monooxygenase structural gene amoa as a functional marker: Molecular fine-scale analysis of natural ammonia-oxidizing populations (1997) Applied and Environmental Microbiology, 63, pp. 4707-4712
  • Rowe, R., Todd, R., Waide, J., Microtechnique for most-probable-number analysis (1977) Applied and Environmental Microbiology, 33, pp. 675-680
  • Sahrawat, K.L., Effects of nitrification inhibitors on nitrogen transformations, other than nitrification, in soils (1989) Advances of Agronomy, 42, pp. 279-309
  • Sala, O.E., Golluscio, R.A., Lauenroth, W.K., Soriano, A., Resource partitioning between shrubs and grasses in the Patagonian steppe (1989) Oecologia, 81, pp. 501-505
  • Sambrook, J., Fritsch, E.F., Maniatis, T., (1989) Molecular Cloning: A Laboratory Manual, , Cold Spring Harbor, New York
  • Schlesinger, W.H., Peterjohn, W.T., Processes controlling ammonia volatilization from Chihuahuan Desert soils (1991) Soil Biology & Biochemistry, 23, pp. 637-642
  • Schlesinger, W.H., Raikes, J.A., Hartley, A.E., Cross, A.F., On the spatial pattern of soil nutrients in desert ecosystems (1996) Ecology, 77, pp. 364-374
  • Soriano, A., Sala, O.E., Perelman, S.B., Patch structure and dynamics in a Patagonian arid steppe (1994) Vegetatio, 111, pp. 127-135
  • Stark, J.M., Firestone, M.K., Kinetic characteristics of ammonium-oxidizer communities in a California oak woodland-annual grassland (1996) Soil Biology & Biochemistry, 28, pp. 1307-1317
  • Valle, H.F.D., Patagonian soils: A regional synthesis (1998) Ecologia Austral, 8, pp. 103-124
  • Vanelli, T., Hooper, A.B., Oxidation of nitrapyrin to 6-chloropiconilic acid by the ammonia-oxidizing bacteria Nitrosomonas europea (1992) Applied and Environmental Microbiology, 58, pp. 2321-2325
  • Ward, B.B., Courtney, K.J., Langeheim, J.H., Inhibition of Nitrosomonas europaea by monoterpenes from coastal redwood (Sequoia sempervirens) in whole-cell studies (1997) Journal of Chemical Ecology, 23, pp. 2583-2598

Citas:

---------- APA ----------
López, N.I., Austin, A.T., Sala, O.E. & Méndez, B.S. (2003) . Controls on nitrification in a water-limited ecosystem: Experimental inhibition of ammonia-oxidising bacteria in the Patagonian steppe. Soil Biology and Biochemistry, 35(12), 1609-1613.
http://dx.doi.org/10.1016/j.soilbio.2003.08.005
---------- CHICAGO ----------
López, N.I., Austin, A.T., Sala, O.E., Méndez, B.S. "Controls on nitrification in a water-limited ecosystem: Experimental inhibition of ammonia-oxidising bacteria in the Patagonian steppe" . Soil Biology and Biochemistry 35, no. 12 (2003) : 1609-1613.
http://dx.doi.org/10.1016/j.soilbio.2003.08.005
---------- MLA ----------
López, N.I., Austin, A.T., Sala, O.E., Méndez, B.S. "Controls on nitrification in a water-limited ecosystem: Experimental inhibition of ammonia-oxidising bacteria in the Patagonian steppe" . Soil Biology and Biochemistry, vol. 35, no. 12, 2003, pp. 1609-1613.
http://dx.doi.org/10.1016/j.soilbio.2003.08.005
---------- VANCOUVER ----------
López, N.I., Austin, A.T., Sala, O.E., Méndez, B.S. Controls on nitrification in a water-limited ecosystem: Experimental inhibition of ammonia-oxidising bacteria in the Patagonian steppe. Soil Biol. Biochem. 2003;35(12):1609-1613.
http://dx.doi.org/10.1016/j.soilbio.2003.08.005