Artículo
Fracchia, S.; Menendez, A.; Godeas, A.; Ocampo, J.A. "A method to obtain monosporic cultures of arbuscular mycorrhizal fungi" (2001) Soil Biology and Biochemistry. 33(9):1283-1285
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Abstract:
We describe here a simple technique for obtaining monosporic cultures of AM fungi. Gigaspora rosea, Gi. sp., Glomus mosseae and G. sp. were used. One surface-sterilized spore was transferred to a 5-cm diameter Petri dish with 10 ml of 10 mM MES buffer, plus 0.04 g of Gel-Gro. The contents of a vermiculite-perlite (1/1, v/v) dish with 2-week-old clover seedlings were transferred onto the Gel-Gro medium with the germinated spore. All plants inoculated with single germinated spores developed mycorrhizas. With this system, non-destructive observation of development of hyphae, formation of fan-like structures, hyphal contact and penetration of AM fungi into the plant root was possible. © 2001 Elsevier Science Ltd. All rights reserved.
Registro:
| Documento: | Artículo |
| Título: | A method to obtain monosporic cultures of arbuscular mycorrhizal fungi |
| Autor: | Fracchia, S.; Menendez, A.; Godeas, A.; Ocampo, J.A. |
| Filiación: | Department Ciencias Biológicas, Universidad De Buenos Aires, 40 II Pabellón, 1428 Buenos Aires, Argentina Department of Microbiology, Estación Experimental del Zaidín, Professor Albareda 1, 18008 Granada, Spain |
| Palabras clave: | Arbuscular mycorrhizas; Gigaspora; Glomus; Monosporic culture; buffer; concentration (parameters); culture vessel; fungus spore; mycorrhiza; plant age; plant fungus interaction; seedling; spore germination; vermiculite; arbuscular mycorrhiza; fungus; sampling; Arbuscular mycorrhizae; Bacteria (microorganisms); Fungi; Gigaspora; Gigaspora rosea; Glomus; Glomus mosseae; Trifolium |
| Año: | 2001 |
| Volumen: | 33 |
| Número: | 9 |
| Página de inicio: | 1283 |
| Página de fin: | 1285 |
| DOI: | http://dx.doi.org/10.1016/S0038-0717(01)00014-1 |
| Título revista: | Soil Biology and Biochemistry |
| Título revista abreviado: | Soil Biol. Biochem. |
| ISSN: | 00380717 |
| CODEN: | SBIOA |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00380717_v33_n9_p1283_Fracchia |
Referencias:
- Becard, G., Piche, Y., Physiological factors determining vesicular-arbuscular mycorrhizal formation in host and nonhost Ri T-DNA transformed roots (1990) Canadian Journal of Botany, 68, pp. 1260-1264
- Bethelenfalvay, G.J., Schuepp, H., Arbuscular mycorrhizas and agrosystem stability (1994) Impact of Arbuscular Mycorrhizas in Sustainable Agriculture and Natural Ecosystems, pp. 117-131. , Gianinazzi, S., Schuepp, H. (Eds.). Birkhauser Verlag, Basel
- Brundrett, M., Juniper, S., Non-destructive assessment of spore germination of VAM fungi and production of pot cultures from single spores (1995) Soil Biology & Biochemistry, 27, pp. 85-91
- Fracchia, S., Mujica, M.T., García-Romera, I., García-Garrido, J.M., Martín, J., Ocampo, J.A., Godeas, A., Interactions between Glomus mosseae and arbuscular mycorrhizal sporocarp-associated saprophytic fungi (1998) Plant and Soil, 200, pp. 131-137
- Gerdemann, J.W., Relation of a large soil-borne spore to phycomycetous mycorrhizal infections (1955) Mycologia, 47, pp. 619-632
- Giovannetti, M., Mosse, B., An evaluation of techniques for measuring vesicular-arbuscular mycorrhizal infection in roots (1980) New Phytologist, 84, pp. 489-500
- Hepper, C.M., Isolation and culture of VA mycorrhizal (VAM) fungi (1984), pp. 95-112. , Powell, C.L., Bagyaraj, D.J. (Eds.). VA Mycorrhiza. CRC Press, Boca Raton, FL; Hewitt, E.J., (1952) Sand water culture methods used in the study of plant nutrition, , Commonwealth Agricultural Bureau, Technical Communication No. 22
- Menge, J.A., Inoculum production (1984) VA Mycorrhiza, pp. 187-204. , Powell, C.L., Bagyaraj, D.J. (Eds.). CRC Press, Boca Raton, FL
- Mosse, B., The establishment of vesicular arbuscular mycorrhiza under aseptic conditions (1962) Journal General Microbiology, 27, pp. 509-520
- Phillips, J.M., Hayman, D.S., Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection (1970) Transactions of the British Mycological Society, 55, pp. 158-161
Citas:
---------- APA ----------
Fracchia, S., Menendez, A., Godeas, A. & Ocampo, J.A. (2001) . A method to obtain monosporic cultures of arbuscular mycorrhizal fungi. Soil Biology and Biochemistry, 33(9), 1283-1285.http://dx.doi.org/10.1016/S0038-0717(01)00014-1
---------- CHICAGO ----------
Fracchia, S., Menendez, A., Godeas, A., Ocampo, J.A. "A method to obtain monosporic cultures of arbuscular mycorrhizal fungi" . Soil Biology and Biochemistry 33, no. 9 (2001) : 1283-1285.http://dx.doi.org/10.1016/S0038-0717(01)00014-1
---------- MLA ----------
Fracchia, S., Menendez, A., Godeas, A., Ocampo, J.A. "A method to obtain monosporic cultures of arbuscular mycorrhizal fungi" . Soil Biology and Biochemistry, vol. 33, no. 9, 2001, pp. 1283-1285.http://dx.doi.org/10.1016/S0038-0717(01)00014-1
---------- VANCOUVER ----------
Fracchia, S., Menendez, A., Godeas, A., Ocampo, J.A. A method to obtain monosporic cultures of arbuscular mycorrhizal fungi. Soil Biol. Biochem. 2001;33(9):1283-1285.http://dx.doi.org/10.1016/S0038-0717(01)00014-1
