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Abstract:

Polyamines, putrescine (PUT), spermidine (SPD), spermine (SPM), and agmatine (AGM), are polycationic amines related to multiple cell functions found in high concentrations during the development of hypothalamus and pituitary. In previous works, we demonstrated that α-difluoromethylornithine (DFMO), an inhibitor of polyamines biosynthesis, induced a delay in puberty of female rats, accompanied by high, sustained follicle-stimulating hormone (FSH) levels during the infantile period. Also, DFMO treatment induced changes in polyamine concentration both in hypothalamus and pituitary of rats, mainly a decrease of PUT and SPD, an increase in SPM, and no change in AGM. In the present work, we investigated the direct effects of polyamines on the secretion of hypothalamic GnRH and pituitary gonadotropins in 6- and 15-day-old female rats. In 6-day-old animals, in vitro incubations with PUT, SPD, and AGM of hypothalami or anterior pituitaries were able to inhibit GnRH, FSH, and leutinizing hormone (LH) secretion, respectively. SPM showed a nonspecific transient inhibitory effect on FSH. When challenged with either high K+ (hypothami) or GnRH (pituitaries), the tissues incubated in the presence of polyamines showed no differences when compared with their controls. No effects of polyamines in 15-day-old rats in either tissue were observed. Pituitary cell cultures of 6-day-old animals incubated with DFMO for 4 days showed a significant increase in FSH, but not in LH. We conclude that high PUT, SPD, and AGM levels during the first 10 days of life are important for the development of the hypothalamic-hypophyseal unit, probably related to an inhibitory effect on GnRH and gonadotropins. Therefore, polyamine participation, especially PUT and SPD, is of importance in the regulation of GnRH and gonadotropin secretion in the neonatal and infantile periods, critical stages in the establishment of sexual differentiation.

Registro:

Documento: Artículo
Título:Effects of polyamines on the release of gonadotropin-releasing hormone and gonadotropins in developing female rats
Autor:Thyssen, S.M.; Hockl, P.F.; Chamson, A.; Lux-Lantos, V.A.R.; Libertun, C.
Filiación:Laboratorio de Neuroendocrinología, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, V. Obligado 2490, 1428 Buenos Aires, Argentina
Departmento Physiology, University of Buenos Aires, Medical School, Argentina
Laboratorio de Neuroendocrinología, Instituto de Biología y Medicina Experimental, V. Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Adenohypophysis; GnRH; Gonadotropins; Hypothalamus; Polyamines; agmatine; eflornithine; follitropin; gonadorelin; gonadotropin; luteinizing hormone; polyamine; putrescine; spermidine; spermine; adenohypophysis; animal cell; animal experiment; article; cell culture; controlled study; development; female; hormone release; hypothalamus; nonhuman; radioimmunoassay; sex differentiation; Animals; Cells, Cultured; Eflornithine; Female; Gonadotropin-Releasing Hormone; Gonadotropins; Pituitary Gland; Polyamines; Pregnancy; Radioimmunoassay; Rats; Rats, Sprague-Dawley; Animalia
Año:2002
Volumen:227
Número:4
Página de inicio:276
Página de fin:281
Título revista:Experimental Biology and Medicine
Título revista abreviado:Exp. Biol. Med.
ISSN:00379727
CODEN:EBMMB
CAS:Eflornithine, 70052-12-9; Gonadotropin-Releasing Hormone, 33515-09-2; Gonadotropins; Polyamines
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00379727_v227_n4_p276_Thyssen

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Citas:

---------- APA ----------
Thyssen, S.M., Hockl, P.F., Chamson, A., Lux-Lantos, V.A.R. & Libertun, C. (2002) . Effects of polyamines on the release of gonadotropin-releasing hormone and gonadotropins in developing female rats. Experimental Biology and Medicine, 227(4), 276-281.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00379727_v227_n4_p276_Thyssen [ ]
---------- CHICAGO ----------
Thyssen, S.M., Hockl, P.F., Chamson, A., Lux-Lantos, V.A.R., Libertun, C. "Effects of polyamines on the release of gonadotropin-releasing hormone and gonadotropins in developing female rats" . Experimental Biology and Medicine 227, no. 4 (2002) : 276-281.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00379727_v227_n4_p276_Thyssen [ ]
---------- MLA ----------
Thyssen, S.M., Hockl, P.F., Chamson, A., Lux-Lantos, V.A.R., Libertun, C. "Effects of polyamines on the release of gonadotropin-releasing hormone and gonadotropins in developing female rats" . Experimental Biology and Medicine, vol. 227, no. 4, 2002, pp. 276-281.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00379727_v227_n4_p276_Thyssen [ ]
---------- VANCOUVER ----------
Thyssen, S.M., Hockl, P.F., Chamson, A., Lux-Lantos, V.A.R., Libertun, C. Effects of polyamines on the release of gonadotropin-releasing hormone and gonadotropins in developing female rats. Exp. Biol. Med. 2002;227(4):276-281.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00379727_v227_n4_p276_Thyssen [ ]