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Abstract:

Polyamines play an essential role in tissue growth and differentiation, in body weight increment, in brain organization, and in the molecular mechanisms of hormonal action, intracellular signaling, and cell-to-cell communication. In a previous study, inhibition of their synthesis by α- difluoromethylornithine (DFMO), a specific and irreversible inhibitor of ornithine decarboxylase, during development in female rats, was followed by prolonged high follicle-stimulating hormone (FSH) serum level and a delayed puberty onset. Those changes were relatively independent of body mass and did not impair posterior fertility. The present work studies the mechanisms and site of action of polyamine participation in FSH secretion during development. DFMO was injected in female rats between Days 1 and 9 on alternate days. At 10 days of age, hypothalami from control and DFMO rats were perifused in vitro, and basal and potassium-induced gonadotropin- releasing hormone (GnRH) release were measured. The response to membrane depolarization was altered in DFMO hypothalami. Increased GnRH release in response to a low K + concentration was evidenced. Adenohypophyses of the same treated prepubertal rats were perifused in vitro and the response to GnRH pulses was checked. In DFMO-treated rats, higher FSH release was observed, with no changes in LH or PRL secretion. Finally, pituitary GnRH receptor number in adenohypophyseal membranes from treated and control groups was quantified. A significant reduction in specific binding was evident in hypophyses from DFMO-treated rats when compared with binding in the control group. In summary, DFMO treatment in a critical developmental period in the female rat impacts the immature GnRH neuronal network and immature gonadotropes. A delay in maturation is evidenced by a higher sensitivity to secretagogs in both pituitary glands and hypothalamic explants. These events could explain the prolonged high FSH serum levels and delayed puberty onset seen in this experimental model.

Registro:

Documento: Artículo
Título:α-Difluoromethylornithine modifies gonadotropin-releasing hormone release and follicle-stimulating hormone secretion in the immature female rat
Autor:Thyssen, S.M.; Becú-Villalobos, D.; Lacau-Mengido, I.M.; Libertun, C.
Filiación:UBA, Argentina
CONICET, Argentina
Laboratorio de Neuroendocrinologia, Instituto de Biologia y Medicina Experimental, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Laboratory of Neuroendocrinology, Institute of Biology and Experimental Medicine-CONICET, 1428 Buenos Aires, Argentina
Palabras clave:eflornithine; follitropin; gonadorelin; luteinizing hormone; polyamine; prolactin; animal experiment; animal model; animal tissue; article; controlled study; delayed puberty; female; follitropin blood level; gonadorelin release; immaturity; luteinizing hormone release; nonhuman; prolactin release; rat; Animals; Eflornithine; Female; Follicle Stimulating Hormone; Gonadotropin-Releasing Hormone; Luteinizing Hormone; Pituitary Gland, Anterior; Potassium; Prolactin; Rats; Rats, Sprague-Dawley; Receptors, LHRH; Secretory Rate; Animalia
Año:1997
Volumen:215
Número:2
Página de inicio:192
Página de fin:197
Título revista:Proceedings of the Society for Experimental Biology and Medicine
Título revista abreviado:PROC. SOC. EXP. BIOL. MED.
ISSN:00379727
CODEN:PSEBA
CAS:eflornithine, 67037-37-0, 70052-12-9; follitropin, 9002-68-0; gonadorelin, 33515-09-2, 9034-40-6; luteinizing hormone, 39341-83-8, 9002-67-9; prolactin, 12585-34-1, 50647-00-2, 9002-62-4; Eflornithine, 70052-12-9; Follicle Stimulating Hormone, 9002-68-0; Gonadotropin-Releasing Hormone, 33515-09-2; Luteinizing Hormone, 9002-67-9; Potassium, 7440-09-7; Prolactin, 9002-62-4; Receptors, LHRH
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00379727_v215_n2_p192_Thyssen

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Citas:

---------- APA ----------
Thyssen, S.M., Becú-Villalobos, D., Lacau-Mengido, I.M. & Libertun, C. (1997) . α-Difluoromethylornithine modifies gonadotropin-releasing hormone release and follicle-stimulating hormone secretion in the immature female rat. Proceedings of the Society for Experimental Biology and Medicine, 215(2), 192-197.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00379727_v215_n2_p192_Thyssen [ ]
---------- CHICAGO ----------
Thyssen, S.M., Becú-Villalobos, D., Lacau-Mengido, I.M., Libertun, C. "α-Difluoromethylornithine modifies gonadotropin-releasing hormone release and follicle-stimulating hormone secretion in the immature female rat" . Proceedings of the Society for Experimental Biology and Medicine 215, no. 2 (1997) : 192-197.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00379727_v215_n2_p192_Thyssen [ ]
---------- MLA ----------
Thyssen, S.M., Becú-Villalobos, D., Lacau-Mengido, I.M., Libertun, C. "α-Difluoromethylornithine modifies gonadotropin-releasing hormone release and follicle-stimulating hormone secretion in the immature female rat" . Proceedings of the Society for Experimental Biology and Medicine, vol. 215, no. 2, 1997, pp. 192-197.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00379727_v215_n2_p192_Thyssen [ ]
---------- VANCOUVER ----------
Thyssen, S.M., Becú-Villalobos, D., Lacau-Mengido, I.M., Libertun, C. α-Difluoromethylornithine modifies gonadotropin-releasing hormone release and follicle-stimulating hormone secretion in the immature female rat. PROC. SOC. EXP. BIOL. MED. 1997;215(2):192-197.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00379727_v215_n2_p192_Thyssen [ ]