Artículo

Ceriani-Nakamurakare, E.; Ramos, S.; Robles, C.A.; Novas, M.V.; D’jonsiles, M.F.; Gonzalez-Audino, P.; Carmarán, C. "Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae)" (2018) Silva Fennica. 52(3)
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Megaplatypus mutatus is a major forest pest in Argentina and an emerging pest in Europe. In this study the multitrophic interactions between M. mutatus and associated fungi were assessed with a metagenomics approach (454-pyrosequencing). A total of 270 collection points from insect galleries from three locations in Argentina were pooled for pyrosequencing analyses. Two hosts, Populus deltoides and Casuarina cunninghamiana, were independently evaluated to characterize the fungal communities associated to M. mutatus; compare the culture-independent approach with previous culturing studies, in terms of data recovery related to the fungal community composition, and test the specificity of the fungal communities amongst locations and hosts. A Generalized Linear Mixed Model was performed to compare the fungal richness in each dataset, which showed no significant differences between taxa richness amongst locations. Principal Coordinates Analyses showed a separation between fungal communities within the same host, suggesting that host identity would not be crucial to determine the specificity in fungal communities. Candida insectalens and one Fusarium species, present in all hosts and locations, achieved 37.6% of the total relative frequency per taxa. These results complement the data from culturing methods previously reported, thus improving the accuracy and understanding of the fungal assemblages associated to M. mutatus. © 2018, Finnish Society of Forest Science. All rights reserved.

Registro:

Documento: Artículo
Título:Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae)
Autor:Ceriani-Nakamurakare, E.; Ramos, S.; Robles, C.A.; Novas, M.V.; D’jonsiles, M.F.; Gonzalez-Audino, P.; Carmarán, C.
Filiación:Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Depto. Biodiversidad y Biología Experimental, Buenos Aires, Argentina
CONICET-Universidad de Buenos Aires, Instituto de Micología y Botánica (INMIBO), Buenos Aires, C1428EHA, Argentina
Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria ConcordiaEntre Ríos E3200, Argentina
Centro de Investigaciones de Plagas e Insecticidas (CITEFA-CONICET), Buenos Aires, B1603ALO, Argentina
Palabras clave:Ambrosia; Forest pest; Fungi; Insect-fungus interactions; Metagenomic; Platypodinae; Cotton; Forestry; Location; Ambrosia; Forest pest; Fungal community compositions; Generalized linear mixed models; Metagenomic; Platypodinae; Principal coordinates analysis; Relative frequencies; Fungi; beetle; community structure; deciduous tree; fungus; genetic analysis; genomics; host plant; host specificity; species richness; Argentina; Casuarina; Cotton; Data; Forestry; Fungi; Populus Deltoides; Test Methods; Argentina; Europe; Ambrosia; Candida insectalens; Casuarina cunninghamiana; Coleoptera; Fungi; Fusarium; Hexapoda; Platypodini; Populus deltoides
Año:2018
Volumen:52
Número:3
DOI: http://dx.doi.org/10.14214/sf.9940
Título revista:Silva Fennica
Título revista abreviado:Silva Fenn.
ISSN:00375330
CODEN:SIFEA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00375330_v52_n3_p_CerianiNakamurakare

Referencias:

  • Adams, A.S., Aylward, F.O., Adams, S.M., Erbilgin, N., Aukema, B.H., Currie, C.R., Suen, G., Raffa, K.F., Mountain pine beetles colonizing historical and naive host trees are associated with a bacterial community highly enriched in genes contributing to terpene metabolism (2013) Applied and Environmental Microbiology, 79, pp. 3468-3475. , https://doi.org/10.1128/aem.00068-13
  • Alfaro, R.I., Humble, L.M., Gonzalez, P., Villaverde, R., Allegro, G., The threat of the ambrosia beetle Megaplatypus mutatus (Chapuis) (=Platypus mutatus Chapuis) to world poplar resources (2007) Forestry, 80, pp. 471-479. , https://doi.org/10.1093/forestry/cpm029
  • Allegro, G., della Beffa, G., Un nuovo problema entomologico per la pioppicoltura italiana: Platypus mutatus Chapuis (Coleoptera, Platypodidae) (2001) Sherwood Foreste Ed Alberi Oggi, 66, pp. 31-34. , A new entomological problem for poplar growing in Italy: Platypus mutatus Chapuis (Coleoptera, Platypodidae)
  • Altschul, S.F., Gish, W., Miller, W., Myers, E.W., Lipman, D.J., Basic local alignment search tool (1990) Journal of Molecular Biology, 215, pp. 403-410. , https://doi.org/10.1006/jmbi.1990.9999
  • Bascialli, M., Giménez, R., Etiennot, A., Toscani, H., Manejo de la población de Platypus sulcatus Chap. Durante tres años en la región delta del Paraná, mediante control químico. [Management of the population of Platypus sulcatus Chap. for three years in the delta region of Paraná, through chemical control] (1996) Investigaciones Agrícolas, Sistema De Recursos Forestales, 5, pp. 129-140
  • Bates, D., Mächler, M., Bolker, B., Walker, S., Fitting linear mixed-effects models using lme4 (2015) Journal of Statistical Software, 67, pp. 1-48. , https://doi.org/10.18637/jss.v067.i01
  • Batra, L.R., Ecology of ambrosia fungi and their dissemination by beetles (1963) Transactions of the Kansas Academy of Science, 66, pp. 213-236. , https://doi.org/10.2307/3626562
  • Batra, L.R., Ambrosia fungi: Extent of specificity to ambrosia beetles (1966) Science, 153, pp. 193-195. , https://doi.org/10.1126/science.153.3732.193
  • Belhoucine, L., Bouhraoua, R.T., Meijer, M., Houbraken, J., Harrak, M.J., Samson, R.A., Equihua-Mar-Tinez, A., Pujade-Villar, J., Mycobiota associated with Platypus cylindrus (Coleoptera: Curculionidae, Platypodidae) in cork oak stands of north west Algeria, Africa (2011) African Journal of Microbiology Research, 5, pp. 4411-4423. , https://doi.org/10.5897/ajmr11.614
  • Bellahirech, A., Inácio, M.L., Bonifácio, L., Nóbrega, F., Sousa, E., Ben Jamâa, M., Comparison of fungi associated with Platypus cylindrus F. (Coleoptera: Platypodidae) in Tunisian and Portuguese cork oak stands (2014) Iobc/Wprs Bulletin, 101, pp. 149-156
  • Bobrowsky, P.T., (2013) Encyclopedia of Natural Hazards, p. 1135. , https://doi.org/10.1007/978-1-4020-4399-4, Springer, New York
  • Boyd, I., Freer-Smith, P., Gilligan, C., Godfray, H., The consequence of tree pests and diseases for ecosystem services (2013) Science, 342, p. 1235773. , https://doi.org/10.1126/science.1235773
  • Breslow, N.E., Clayton, D.G., Approximate inference in generalized linear mixed models (1993) Journal of American Statistical Association, 88, pp. 9-25. , https://doi.org/10.1080/01621459.1993.10594284
  • Caporaso, J.G., Kuczynski, J., Stombaugh, J., Bittinger, K., Buschman, F.D., Costello, E.K., QIIME allows analysis of high-throughput community sequencing data (2010) Nature Methods, 7, pp. 335-336. , https://doi.org/10.1038/nmeth.f.303
  • Castrillo, L.A., Griggs, M.H., Vandenberg, J.D., Competition between biological control fungi and fungal symbionts of ambrosia beetles Xylosandrus crassiusculus and X. Germanus (Coleoptera: Curculionidae): Mycelial interactions and impact on beetle brood production (2016) Biological Control, 103, pp. 138-146. , https://doi.org/10.1016/j.biocontrol.2016.09.005
  • Ceriani-Nakamurakare, E., Slodowicz, M., Gonzalez-Audino, P., Dolinko, A., Carmarán, C., Mycobiota associated with the ambrosia beetle Megaplatypus mutatus: Threat to poplar plantations (2016) Forestry, 89, pp. 191-200. , https://doi.org/10.1093/forestry/cpw001
  • Charles, J., Nef, L., Allegro, G., Collins, C.M., Delplanque, A., Gímenez, R., Höglund, S., Augustin, S., Insect and other pests of poplars and willows (2014) Poplars and Willows: Trees for Society and the Environment, pp. 459-526. , https://doi.org/10.1079/9781780641089.0459, Isebrands J.G., Richardon J. (eds.), CABI, Oxford
  • Chatterjee, S., Kuang, Y., Splivallo, R., Chatterjee, P., Karlovsky, P., Interactions among filamentous fungi Aspergillus niger, Fusarium verticillioides and Clonostachys rosea: Fungal biomass, diversity of secreted metabolites and fumonisin production (2016) BMC Microbiology, 16 (83). , https://doi.org/10.1186/s12866-016-0698-3
  • Colwell, R.K., EstimateS, Version 9.1: Statistical estimation of species richness and shared species from samples (Software and User’s Guide) (2013) Freeware for Windows and Mac OS, , http://viceroy.uconn.edu/estimates/index.html
  • Cruywagen, E.M., de Beer, Z.W., Roux, J., Wingfield, M.J., Three new Graphium species from baobab trees in South Africa and Madagascar (2010) Persoonia-Molecular Phylogeny and Evolution of Fungi, 25, pp. 61-71. , https://doi.org/10.3767/003158510x550368
  • Dighton, J., White, J.F., Jr., White, J., Oudemans, P., (2005) The Fungal Community: Its Organization and Role in the Ecosystem, p. 960. , https://doi.org/10.1201/9781420027891, 3rd ed. CRC Press, New York
  • Dreistadt, S.H., Dahlsten, D.L., Frankie, G.W., Urban forests and insect ecology (1990) Bioscience, 40, pp. 192-198. , https://doi.org/10.2307/1311364
  • Endoh, R., Suzuki, M., Benno, Y., Futai, K., Candida kashinagacola sp. Nov., C. pseudovander-kliftii sp. nov. and C. vanderkliftii sp. nov., three new yeasts from ambrosia beetle-associated sources (2008) Antonie Van Leeuwenhoek, 94, pp. 389-402. , https://doi.org/10.1007/s10482-008-9256-9
  • Endoh, R., Suzuki, M., Okada, G., Takeuchi, Y., Futai, K., Fungus symbionts colonizing the galleries of the ambrosia beetle Platypus quercivorus (2011) Microbial Ecology, 62, pp. 106-120. , https://doi.org/10.1007/s00248-011-9838-3
  • Eslyn, W.E., Lombard, F.F., Fungi associated with decayed wood in stored willow and cottonwood logs (1984) Mycologia, 76, pp. 548-550. , https://doi.org/10.2307/3793339
  • Eyjolfsdottir, G.G., (1990) Fungi Isolated from Stained Wood Associated with Bark Beetle Galleries in Timber Trees in New Zealand, Norway and Western Canada, , Ph.D. thesis, University of Manitoba
  • Fraedrich, S., Harrington, T., Rabaglia, R., Ulyshen, M.D., Mayfield, A.E., III, Hanula, J.L., Eickwort, J.M., Miller, D.R., A fungal symbiont of the redbay ambrosia beetle causes a lethal wilt in redbay and other Lauraceae in the southeastern United States (2008) Plant Disease, 92, pp. 215-224. , https://doi.org/10.1094/pdis-92-2-0215
  • Francke-Grosmann, H., Skin glands as a carrier of fungal symbiosis in ambrosia beetles (1956) Zeitschrift für Morphologie Und Öekologie Der Tiere, 45, pp. 275-308
  • Francke-Grosmann, H., Ectosymbiosis in wood-inhabiting insects (1967) Symbiosis, 2, pp. 141-205. , https://doi.org/10.1016/b978-1-4832-2758-0.50010-2
  • Freeman, S., Sharon, M., Maymon, M., Mendel, Z., Protasov, A., Aoki, T., Eskalen, A., O’do-Nell, K., Fusarium euwallaceae sp. Nov. a symbiotic fungus of Euwallacea sp., an invasive ambrosia beetle in Israel and California (2013) Mycologia, 105, pp. 1595-1606. , https://doi.org/10.3852/13-066
  • Funes, H., Griffo, R., Zerba, E., Gonzalez-Audino, P., Mating disruption of the ambrosia beetle Megaplatypus mutatus in poplar and hazelnut plantations using reservoir systems for pheromones (2011) Entomologia Experimentalis Et Applicata, 139, pp. 226-234. , https://doi.org/10.1111/j.1570-7458.2011.01126.x
  • Gazis, R., Chaverri, P., Diversity of fungal endophytes in leaves and stems of wild rubber trees (Hevea brasiliensis) in Peru (2010) Fungal Ecology, 3 (3), pp. 240-254. , https://doi.org/10.1016/j.funeco.2009.12.001
  • Gebhardt, H., Begerow, D., Oberwinkler, F., Identification of the ambrosia fungus of Xyle-borus monographus and X. Dryographus (Coleoptera: Curculionidae, Scolytinae) (2004) Mycological Progress, 3, pp. 95-102. , https://doi.org/10.1007/s11557-006-0080-1
  • Giménez, R.A., Etiennot, A.E., Host range of Platypus mutatus (Chapuis, 1865) (Coleoptera: Platypodidae) (2007) Entomotropica, 18, pp. 89-94
  • González-Audino, P., Gatti, P., Zerba, E., Traslucent pheromone traps increase trapping efficiency of ambrosia beetle Megaplatypus mutatus (2011) Crop Protection, 30, pp. 745-747. , https://doi.org/10.1016/j.cropro.2011.02.008
  • Guarro, J., Vieira, L.A., de Freitas, D., Gené, J., Zaror, L., Hofling-Lima, A.L., Fischman, O., Figueras, M.J., Phaeoisaria clematidis as a cause of keratomycosis (2000) Journal of Clinical Microbiology, 38, pp. 2434-2437
  • Hennessey, T.C., Dougherty, P.M., Kossuth, S.V., Johnson, J.D., (1986) Stress Physiology and Forest Productivity: Proceedings of the Physiology Working Group Technical Session, p. 239. , https://doi.org/10.1007/978-94-009-4424-4, Martinus Nijhoff Publishers, Dordrecht
  • Henry, S.M., (1967) Symbiosis: Associations of Invertebrates, Birds, Ruminants, and Other Biota, p. 462. , Academic Press, New York & London
  • Hiergeist, A., Gläsner, J., Reischl, U., Gessner, A., Analyses of intestinal microbiota: Culture versus sequencing (2015) ILAR Journal, 56, pp. 228-240. , https://doi.org/10.1093/ilar/ilv017
  • Hiraoka, S., Yang, C., Iwasaki, W., Metagenomics and bioinformatics in microbial ecology: Current status and beyond (2016) Microbes Environment, 31, pp. 204-212. , https://doi.org/10.1093/ilar/ilv017
  • Hu, X., Li, M., Chen, H., Community structure of gut fungi during different developmental stages of the Chinese white pine beetle (Dendroctonus armandi) (2015) Scientific Reports, 5, p. 8411. , https://doi.org/10.1038/srep08411
  • Hulcr, J., Dunn, R.R., The sudden emergence of pathogenicity in insect–fungus symbioses threatens naive forest ecosystems (2011) Proceedings of the Royal Society of London: Biological Sciences, 278 (1720), pp. 2866-2873. , https://doi.org/10.1098/rspb.2011.1130
  • Hulcr, J., Stelinski, L.L., The ambrosia symbiosis: From evolutionary ecology to practical management (2016) Annual Review Entomology, 62, pp. 285-303. , https://doi.org/10.1146/annurev-ento-031616-035105
  • Hulcr, J., Kolarik, M., Kirkendall, L.R., A new record of fungus-beetle symbiosis in Scoly-todes bark beetles (Scolytinae, Curculionidae, Coleoptera) (2007) Symbiosis, 43, p. 151
  • Hulme, P.E., Trade, transport and trouble: Managing invasive species pathways in an era of globalization (2009) Journal of Applied Ecology, 46, pp. 10-18. , https://doi.org/10.1111/j.1365-2664.2008.01600.x
  • Kasson, M.T., O’Donnell, K., Rooney, A.P., Sink, S., Ploetz, R.C., Ploetz, J.N., Konkol, J.L., Geiser, D.M., An inordinate fondness for Fusarium: Phylogenetic diversity of fusaria cultivated by ambrosia beetles in the genus Euwallacea on avocado and other plant hosts (2013) Fungal Genetics and Biology, 56, pp. 147-157. , https://doi.org/10.1016/j.fgb.2013.04.004
  • Kelt, D.A., Meserve, P.L., Status and challenges for conservation of small mammal assemblages in South America (2014) Biological Reviews Cambridge Philosophical Society, 89, pp. 705-722. , https://doi.org/10.1111/brv.12080
  • Kimura, N., Metagenomics: Access to unculturable microbes in the environment (2006) Microbes Environment, 21, pp. 201-215. , https://doi.org/10.1264/jsme2.21.201
  • Kolařík, M., Hulcr, J., Kirkendall, L.R., New species of Geosmithia and Graphium associated with ambrosia beetles in Costa Rica (2015) Czech Mycology, 67, pp. 29-35
  • Kostovcik, M., Bateman, C.C., Kolarik, M., Stelinski, L.L., Jordal, B.H., Hulcr, J., The ambrosia symbiosis is specific in some species and promiscuous in others: Evidence from community pyrosequencing (2015) The ISME Journal, 9, pp. 126-138. , https://doi.org/10.1038/ismej.2014.115
  • Kunin, V., Engelbrektson, A., Ochman, H., Hugenholtz, P., Wrinkles in the rare biosphere: Pyrosequencing errors can lead to artificial inflation of diversity estimates (2010) Environmental Microbiology, 12, pp. 118-123. , https://doi.org/10.1111/j.1462-2920.2009.02051.x
  • Liebhold, A.M., Macdonald, W.L., Bergdahl, D., Mastro, V.C., Invasion by exotic forest pests: A threat to forest ecosystems (1995) Forest Science, 41, pp. a0001-z0001
  • Martínez-García, L.B., Richardson, S.J., Tylianakis, J.M., Peltzer, D.A., Dickie, I.A., Host identity is a dominant driver of mycorrhizal fungal community composition during ecosystem development (2015) New Phytologist, 205, pp. 1565-1576. , https://doi.org/10.1111/nph.13226
  • Miller, K.E., Hopkins, K., Inward, D.J., Vogler, A.P., Metabarcoding of fungal communities associated with bark beetles (2016) Ecology and Evolution, 6, pp. 1590-1600. , https://doi.org/10.1002/ece3.1925
  • Nilsson, R.H., Tedersoo, L., Ryberg, M., Kristiansson, E., Hartmann, M., Unterseher, M., Porter, T.M., Abarenkov, K., A comprehensive, automatically updated fungal ITS sequence dataset for reference-based chimera control in environmental sequencing efforts (2015) Microbes Environment, 30, pp. 145-150. , https://doi.org/10.1264/jsme2.me14121
  • Orgiazzi, A., Bianciotto, V., Bonfante, P., Daghino, S., Ghignon, S., Lazzari, A., Lumini, E., Girlanda, M., 454 pyrosequencing analysis of fungal assemblages from geographically distant, disparate soils reveals spatial patterning and a core mycobiome (2013) Diversity, 5, pp. 73-98. , https://doi.org/10.3390/d5010073
  • Pérez‐Izquierdo, L., Zabal‐Aguirre, M., Flores‐Rentería, D., González‐Martínez, S.C., Buée, M., Rincón, A., Functional outcomes of fungal community shifts driven by tree genotype and spatial‐temporal factors in Mediterranean pine forests (2017) Environmental Microbiology, 19, pp. 1639-1652. , https://doi.org/10.1111/1462-2920.13690
  • Ploetz, R.C., Hulcr, J., Wingfield, M.J., de Beer, Z.W., Destructive tree diseases associated with ambrosia and bark beetles: Black swan events in tree pathology? (2013) Plant Disease, 97, pp. 856-872. , https://doi.org/10.1094/pdis-01-13-0056-fe
  • Ploetz, R.C., Konkol, J.L., Narvaez, T., Duncan, R.E., Saucedo, R.J., Campbell, A., Mantilla, J., Kendra, P.E., Presence and prevalence of Raffaelea lauricola, cause of laurel wilt, in different species of ambrosia beetle in Florida, USA (2017) Journal of Economic Entomology, 110, pp. 347-354. , https://doi.org/10.1093/jee/tow292
  • Popa, V., Déziel, E., Lavallée, R., Bauce, E., Guertin, C., The complex symbiotic relationships of bark beetles with microorganisms: A potential practical approach for biological control in forestry (2012) Pest Management Science, 68 (7), pp. 963-975. , https://doi.org/10.1002/ps.3307
  • Qi, H., Wang, J., Endoh, R., Takeuchi, Y., Tarno, H., Futai, K., Pathogenicity of microorganisms isolated from the oak platypodid, Platypus quercivorus (Murayama) (Coleoptera: Platypo-didae) (2011) Applied Entomology and Zoology, 46, pp. 201-210. , https://doi.org/10.1007/s13355-011-0032-3
  • Ramsfield, T., Bentz, B., Faccoli, M., Jactel, H., Brockerhoff, E., Forest health in a changing world: Effects of globalization and climate change on forest insect and pathogen impacts (2016) Forestry, 89, pp. 245-252. , https://doi.org/10.1093/forestry/cpw018
  • Robert, V., Vu, D., Amor, A.B.H., van de Wiele, N., Brouwer, C., Bernard, J., Szoke, S., Crous, P.W., MycoBank gearing up for new horizons (2013) IMA Fungus, 4, pp. 371-379. , https://doi.org/10.5598/imafungus.2013.04.02.16
  • Schimann, H., Bach, C., Lengelle, J., Louisanna, E., Barantal, S., Murat, C., Buée, M., Diversity and structure of fungal communities in neotropical rainforest soils: The effect of host recurrence (2016) Microbial Ecology, 73, pp. 310-320. , https://doi.org/10.1007/s00248-016-0839-0
  • Schoch, C.L., Seifert, K.A., Huhndorf, S., Robert, V., Spouge, J.L., Levesque, C.A., Chen, W., Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi (2012) Proceedings of the National Academy of Sciences of the United States of America, 109 (16), pp. 6241-6246. , https://doi.org/10.1073/pnas.1117018109
  • Scully, E.D., Hoover, K., Carlson, J., Tien, M., Geib, S.M., Proteomic analysis of Fusarium solani isolated from the Asian longhorned beetle, Anoplophora glabripennis (2012) Plos One, 7. , https://doi.org/10.1371/journal.pone.0032990
  • Scully, E.D., Geib, S.M., Carlson, J.E., Tien, M., McKenna, D., Hoover, K., Functional genomics and microbiome profiling of the Asian longhorned beetle (Anoplophora glabripennis) reveal insights into the digestive physiology and nutritional ecology of wood feeding beetles (2014) BMC Genomics, 15, p. 1096. , https://doi.org/10.1186/1471-2164-15-1096
  • Short, D.P., O’Donnell, K., Stajich, J.E., Hulcr, J., Kijimoto, T., Berger, M.C., Macias, A.M., Kasson, M.T., PCR multiplexes discriminate Fusarium symbionts of invasive Euwallacea ambrosia beetles that inflict damage on numerous tree species throughout the United States (2017) Plant Disease, 101, pp. 233-240. , https://doi.org/10.1094/pdis-07-16-1046-re
  • Stefani, F.O., Bell, T.H., Marchand, C., de la Providencia, I.E., El Yassimi, A., St-Arnaud, M., Hijri, M., Culture-dependent and-independent methods capture different microbial community fractions in hydrocarbon-contaminated soils (2015) Plos One, 10. , https://doi.org/10.1371/journal.pone.0128272
  • Sun, X., Guo, L.-D., Hyde, K., Community composition of endophytic fungi in Acer trun-catum and their role in decomposition (2011) Fungal Diversity, 47, pp. 85-95. , https://doi.org/10.1007/s13225-010-0086-5
  • Tremblay, E., Espinosa, B., Mancini, D., Caprio, G., A beetle from South America threatens poplars (2000) Informatore Agrario, 56, pp. 89-90
  • Tsui, C.K., Hyde, K.D., Fukushima, K., Fungi on submerged wood in the Koito River, Japan (2003) Mycoscience, 44, pp. 55-59. , https://doi.org/10.1007/s10267-002-0083-y
  • van der Walt, J., The yeast genus Ambrosiozyma gen. Nov. (Ascomycetes) (1972) Mycopathologia Et Mycologia Applicata, 46, pp. 305-315. , https://doi.org/10.1007/bf02052126
  • White, T.J., Bruns, T., Lee, S., Taylor, J.W., Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics (1990) PCR Protocols: A Guide to Methods and Applications, pp. 315-322. , https://doi.org/10.1016/B978-0-12-372180-8.50042-1, Innis M.A., Gelfand D.H., Sninsky J.J., White T.J. (eds.), Academic Press, San Diego, California
  • Whitton, S.R., McKenzie, E.H., Hyde, K.D., The current understanding of fungi associated with Pandanaceae (2012) Fungal Diversity Research Series, 21, pp. 1-428. , https://doi.org/10.1007/978-94-007-4447-9_1
  • Yun, Y.H., Suh, D.Y., Yoo, H.D., Oh, M.H., Kim, S.H., Yeast associated with the ambrosia beetle, Platypus koryoensis, the pest of oak trees in Korea (2015) Mycobiology, 43, pp. 458-466. , https://doi.org/10.5941/myco.2015.43.4.458
  • Zhou, J., He, Z., Yang, Y., Deng, Y., Tringe, S.G., Alvarez-Cohen, L., High-throughput metagenomic technologies for complex microbial community analysis: Open and closed formats (2015) Mbio, 6, pp. e02288-02214. , https://doi.org/10.1128/mbio.02288-14

Citas:

---------- APA ----------
Ceriani-Nakamurakare, E., Ramos, S., Robles, C.A., Novas, M.V., D’jonsiles, M.F., Gonzalez-Audino, P. & Carmarán, C. (2018) . Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae). Silva Fennica, 52(3).
http://dx.doi.org/10.14214/sf.9940
---------- CHICAGO ----------
Ceriani-Nakamurakare, E., Ramos, S., Robles, C.A., Novas, M.V., D’jonsiles, M.F., Gonzalez-Audino, P., et al. "Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae)" . Silva Fennica 52, no. 3 (2018).
http://dx.doi.org/10.14214/sf.9940
---------- MLA ----------
Ceriani-Nakamurakare, E., Ramos, S., Robles, C.A., Novas, M.V., D’jonsiles, M.F., Gonzalez-Audino, P., et al. "Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae)" . Silva Fennica, vol. 52, no. 3, 2018.
http://dx.doi.org/10.14214/sf.9940
---------- VANCOUVER ----------
Ceriani-Nakamurakare, E., Ramos, S., Robles, C.A., Novas, M.V., D’jonsiles, M.F., Gonzalez-Audino, P., et al. Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae). Silva Fenn. 2018;52(3).
http://dx.doi.org/10.14214/sf.9940